Revisiting endophytic fungi of the Pinus-Viscum-system to assess interdependencies among factors driving community structure.

重新审视松-槲寄生系统的内生真菌，以评估驱动群落结构的因素之间的相互依赖性。

• 批准号: 213130063
• 负责人: Privatdozent Dr. Derek Persoh
• 金额: --
• 依托单位: Arbeitsgruppe Geobotanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/213130063?language=en
• 关键词: Revisiting; endophytic; fungi; Pinus; Viscum

Nearly all plants studied so far are inhabited by a complex assemblage of endophytic fungi. However, the factors driving the composition of these communities are poorly understood. In a previous project we obtained first evidence that the endophytic fungi in leaves and stems differ with regard to diversity, variability, and host-selectivity. Several hypotheses resulted from that study. These hypotheses to be tested in the proposed project are that (a) the stem-inhabiting fungal community is rather poor in species, (b) to a certain degree selective for their host plant species, and (c) does not change through time in adult host plants. In contrast, (d) the diversity within leaves is assumed to increase with increasing leaf age and (e) the spectrum of accumulating fungi is not host-selective. Its composition rather depends on the availability of suitable substrates for fungal sporulation (i.e. decaying plant material) in the surrounding environment and therefore on the composition of the plant community. To verify these hypotheses the previously studied Pinus-Viscum-system is chosen because i) the age of the organs of both plants is easily determinable, ii) both species comprise at least current- and previous-year leaves, and iii) they are exposed to the same spectrum of fungal spores, due to their close proximity. Pyrosequencing will be applied to fungal DNA in each of five replicates of differently aged stems and leaves of each species of the Pinus-Viscum-system and non-infected Pinus trees in addition. The extensive data gathered this way will allow addressing the hypotheses on a statistically sound background. The applicability of the proposed methodology has previously been verified and success of the pyrosequencing run is guaranteed by an external contractor.

迄今为止研究的几乎所有植物都栖息着复杂的内生真菌组合。然而，人们对驱动这些社区组成的因素知之甚少。在之前的项目中，我们获得了第一个证据，表明叶和茎中的内生真菌在多样性、变异性和宿主选择性方面存在差异。该研究得出了几个假设。在拟议项目中要测试的这些假设是：（a）茎栖息真菌群落的物种相当贫乏，（b）对其寄主植物物种具有一定程度的选择性，以及（c）成年寄主植物不会随时间而变化。相比之下，（d）假设叶子内的多样性随着叶龄的增加而增加，并且（e）积累真菌的谱不具有宿主选择性。它的组成相当取决于周围环境中真菌孢子形成的合适基质（即腐烂的植物材料）的可用性，因此取决于植物群落的组成。为了验证这些假设，选择了先前研究的松树 - 槲寄生系统，因为 i）两种植物的器官的年龄很容易确定，ii）两个物种至少包含当年和上一年的叶子，以及 iii）由于它们非常接近，它们暴露于相同范围的真菌孢子。焦磷酸测序将应用于松树-槲寄生系统每个物种和未受感染的松树的不同年龄的茎和叶的五个重复样本中的每一个的真菌DNA。通过这种方式收集的大量数据将有助于在统计上合理的背景下解决假设。所提出方法的适用性先前已得到验证，焦磷酸测序运行的成功由外部承包商保证。