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Collaborative Research: Developmental and functional mechanisms of complex trait re-evolution: Limb loss and gain in skink lizards

合作研究：复杂性状再进化的发育和功能机制：石龙子蜥蜴的肢体损失和增益

基本信息

批准号：
1353683
负责人：
Cameron Siler
金额：
$ 23.01万
依托单位：
University of Oklahoma Norman Campus
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-01 至 2018-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353683&HistoricalAwards=false
关键词：
Collaborative Research Developmental functional mechanisms

项目摘要

Understanding how complex characters evolve is central to developing fundamental theories of trait evolution. One such theory, Dollo's Law, which states that once lost, complex characters cannot be re-evolved, has challenged biologists for more than a century. Despite recent evidence suggesting that complex characters can and do re-evolve, there is a lack of understanding of how and why these evolutionary reversals take place. The proposed study will address these questions by elucidating the functional underpinnings and the developmental mechanisms of complex trait re-evolution. This project will result in an unprecedented integration of functional morphology and developmental genetics in an evolutionary framework, facilitating communication between these fields.The research project will use an integrative approach that synthesizes developmental genetics and functional morphology in a phylogenetic framework to study limb and digit loss and subsequent re-evolution in the Southeast Asian burrowing lizard genus Brachymeles. Objectives of the proposed research are to determine whether a phenotypic trait that has been lost re-evolves with the same or different functional capacities, and whether re-evolved digits develop via the same or different developmental mechanisms as ancestral digits. Surface locomotor velocity, acceleration, and burrowing speed and force will be quantified using high-speed video and a force transducer for ancestrally and re-evolved pentadactyl, intermediate, and limbless species. The developmental basis of limb and digit reduction and re-evolution in Brachymeles will be characterized to identify hypothesized developmental constraints on limb and digit re-evolution. Mesenchymal condensations of digits in embryos will be identified using histochemistry and in situ hybridization of nine genes associated with limb and digit patterning. The project will be also involve and train undergraduate and graduate students in research methods and will promote international collaborations. Minority and female students will be actively recruited and encouraged to participate in the research training opportunities provided by the project. Biologically themed outreach programs resulting from this project will target disadvantaged communities and local K-12 schools. Findings from the proposed project will be publicly disseminated online as part of an established biodiversity information website (http://philbreo.lifedesks.org/), at scientific conferences, in peer-reviewed journals, and through other existing databases (http://datadryad.org/).

理解复杂的特征是如何进化的，这是发展特征进化基本理论的核心。一个多世纪以来，这样的一种理论--多洛定律一直在挑战生物学家。该定律指出，复杂的字符一旦丢失，就不能再进化。尽管最近的证据表明，复杂的字符可以而且确实会重新进化，但对于这些进化逆转是如何以及为什么发生的，人们缺乏了解。这项拟议的研究将通过阐明复杂性状再进化的功能基础和发展机制来解决这些问题。该项目将在进化框架中实现功能形态和发育遗传学的前所未有的整合，促进这些领域之间的交流。该研究项目将使用一种在系统发育框架中综合发育遗传学和功能形态的综合方法来研究东南亚穴居蜥蜴属Brachymeles的肢体和手指的丧失以及随后的再进化。这项拟议研究的目的是确定丢失的表型特征是否以相同或不同的功能能力重新进化，以及重新进化的手指是通过与祖先手指相同或不同的发育机制发展的。地面运动速度、加速度、洞穴速度和力将使用高速视频和力传感器进行量化，用于祖先和重新进化的五手龙、中间物种和无肢物种。将描述短臂类肢体和手指缩小和再进化的发育基础，以确定假想的肢体和手指再进化的发展制约因素。胚胎中的手指间充质凝聚将通过组织化学和与肢体和手指图案相关的9个基因的原位杂交来鉴定。该项目还将在研究方法方面对本科生和研究生进行参与和培训，并将促进国际合作。将积极招募少数民族和女性学生，并鼓励他们参加该项目提供的研究培训机会。由该项目产生的以生物为主题的外展计划将针对弱势社区和当地的K-12学校。拟议项目的调查结果将作为已建立的生物多样性信息网站(http://philbreo.lifedesks.org/)，)的一部分在网上公开传播，并在科学会议、同行评议的期刊上以及通过其他现有数据库(http://datadryad.org/).

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The comparative biogeography of Philippine geckos challenges predictions from a paradigm of climate‐driven vicariant diversification across an island archipelago

菲律宾壁虎的比较生物地理学挑战了气候驱动的整个岛屿群岛的替代多样化范式的预测

DOI：
10.1111/evo.13754
发表时间：
2019
期刊：
Evolution
影响因子：
3.3
作者：
Oaks, Jamie R.;Siler, Cameron D.;Brown, Rafe M.
通讯作者：
Brown, Rafe M.

Use of diet and body condition assessments as intermediate indicators of translocation success in the Critically Endangered Philippine crocodile ( Crocodylus mindorensis )

DOI：
10.1002/aqc.3700
发表时间：
2021-09
期刊：
Aquatic Conservation: Marine and Freshwater Ecosystems
影响因子：
0
作者：
Joseph C. Brown;M. Shirley;Amante Yog‐yog;M. Weerd;Marites G. Balbas;B. A. Tarun;Cameron D. Siler
通讯作者：
Joseph C. Brown;M. Shirley;Amante Yog‐yog;M. Weerd;Marites G. Balbas;B. A. Tarun;Cameron D. Siler

Multilocus phylogeny and a new classification for African, Asian and Indian supple and writhing skinks (Scincidae: Lygosominae)

非洲、亚洲和印度柔软扭动石龙子的多位点系统发育和新分类（石龙子科：Lygosominae）

DOI：
10.1093/zoolinnean/zlz001
发表时间：
2019
期刊：
Zoological Journal of the Linnean Society
影响因子：
2.8
作者：
Freitas, Elyse S;Datta-Roy, Aniruddha;Karanth, Praveen;Grismer, L Lee;Siler, Cameron D
通讯作者：
Siler, Cameron D

A taxonomic conundrum: Characterizing a cryptic radiation of Asian gracile skinks (Squamata: Scincidae: Riopa) in Myanmar