Photoprotection in Diverse, Desiccation-Tolerant, Desert Green Algae and Their Close Aquatic Relatives

多种耐干燥沙漠绿藻及其近亲水生植物的光保护

基本信息

  • 批准号:
    1355085
  • 负责人:
  • 金额:
    $ 63万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2014
  • 资助国家:
    美国
  • 起止时间:
    2014-08-01 至 2019-07-31
  • 项目状态:
    已结题

项目摘要

The objective of this research is to determine the process(es) underlying the mechanism(s) protecting the photosynthetic apparatus of desert green algae during desiccation and rehydration. A key step in the evolution of life on Earth was the move from living in water to surviving on land. Organisms making that leap faced high light levels, extreme temperatures, and desiccation. Flowering plants, conifers, mosses, and ferns are all descended from a single green algal ancestor that successfully invaded land. Traits they share may be essential for surviving on land, or they might simply be traits inherited from the common ancestor. To tease apart these two possibilities, other green, photosynthetic, terrestrial organisms must be studied that are not descended from that common green algal ancestor. Such organisms are found among diverse, free-living, microscopic green algae inhabiting microbiotic crusts of the desert Southwestern U.S. Desert green algae survive desiccation much better than their aquatic algal relatives, and desert algae recover from desiccation very rapidly (within minutes) when rehydrated. Thus, they make an ideal system to study basic mechanisms of desiccation tolerance. The traits land plants developed to protect themselves from intense sunlight during drought, high heat, and cold are particularly important as agriculture expands into marginal lands while climate also shifts. Desert green algae share many characteristics with larger agriculturally and ecologically important green plants, and also with the green algae increasingly used for biofuel production. Research integration with education and training will include development of 6-8th grade Lesson Plans, available to teachers leading excursions at Cape Cod National Seashore focusing on "Surprising Microbes of the Dunes". Research opportunities for undergraduates will be focused on projects studying desiccation stress and photobiology in green plants and microbiotic crusts.The overall research objective is to determine the process(es) underlying (and phylogenetic distribution of) rapidly-reversible mechanism(s) protecting the photosynthetic apparatus during desiccation and rehydration, in paired desert and aquatic green algae within four algal classes. Broad phylogenetic distribution of photoprotective or desiccation-tolerance mechanisms across desert members of multiple green algal classes will suggest (through parsimony) that these mechanisms are likely ancestral traits in the larger green plant clade. Comparison of traits in desert and closely-related aquatic relatives will identify lineage-specific characteristics. Research will establish whether the capacity for desiccation tolerance and desiccation-induced photoprotection has a persistent genetic component (even when algae are grown hydrated), and whether influences of light and speed of desiccation on the efficiency of photoprotection are similar to their effects in lichens and mosses. Mechanisms that have been recently proposed to underlie desiccation-induced photoprotection (PSII to PSI spillover, P700+ oxidation, and chlorophyll-chlorophyll charge transfer states) will be tested in the lichen Parmelia sulcata and desiccation-tolerant green algae using fluorescence decay kinetics and modeling, EPR, and linear and circular dichroism spectroscopy. Whole transcriptome shotgun sequencing will be used to test whether, as in bryophytes, the transcriptome in the representative desert-derived green alga Scenedesmus rotundus possesses constititive cellular protection against desiccation.
本研究的目的是确定沙漠绿藻在干燥和补水过程中保护光合装置的机制背后的过程。 地球生命进化的关键一步是从水中生存到陆地上生存。实现这一飞跃的生物体面临着强光、极端温度和干燥。开花植物、针叶树、苔藓和蕨类植物都是成功入侵陆地的单一绿藻祖先的后代。它们共有的特征可能对于在陆地上生存至关重要,或者它们可能只是从共同祖先继承的特征。 为了区分这两种可能性,必须研究并非来自共同绿藻祖先的其他绿色光合陆生生物。这种生物体存在于美国西南部沙漠微生物结皮中的各种自由生活的微观绿藻中。沙漠绿藻在干燥条件下的生存能力比其水生藻类亲戚要好得多,并且沙漠藻类在重新水合后从干燥状态中恢复得非常快(几分钟内)。因此,他们构建了一个研究耐干燥基本机制的理想系统。随着农业向边际土地的扩展以及气候的变化,陆地植物为在干旱、高温和寒冷期间保护自己免受强烈阳光照射而形成的特性尤为重要。沙漠绿藻与大型农业和生态重要的绿色植物以及越来越多地用于生物燃料生产的绿藻具有许多共同特征。 研究与教育和培训的整合将包括制定 6-8 年级课程计划,供带领科德角国家海岸游览的教师使用,重点关注“沙丘上令人惊奇的微生物”。 本科生的研究机会将集中于研究绿色植物和微生物结皮中的干燥应激和光生物学的项目。总体研究目标是确定四种藻类中成对的沙漠绿藻和水生绿藻在干燥和补水过程中保护光合机构的快速可逆机制的潜在过程(及其系统发育分布)。 多个绿藻类别的沙漠成员中光保护或耐干燥机制的广泛系统发育分布表明(通过简约)这些机制可能是较大绿色植物进化枝中的祖先特征。比较沙漠中的性状和密切相关的水生亲属将确定谱系特定的特征。研究将确定干燥耐受性和干燥诱导的光保护能力是否具有持久的遗传成分(即使藻类在水合条件下生长),以及光和干燥速度对光保护效率的影响是否与它们对地衣和苔藓的影响相似。 最近提出的干燥诱导光保护机制(PSII 到 PSI 溢出、P700+ 氧化和叶绿素-叶绿素电荷转移状态)将使用荧光衰变动力学和建模、EPR 以及线性和圆二色性在地衣 Parmelia sulcata 和耐干燥绿藻中进行测试 光谱学。 全转录组鸟枪法测序将用于测试代表性沙漠绿藻圆栅藻的转录组是否像苔藓植物一样具有针对干燥的组成性细胞保护作用。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A model suite of green algae within the Scenedesmaceae for investigating contrasting desiccation tolerance and morphology
  • DOI:
    10.1242/jcs.212233
  • 发表时间:
    2018-04-01
  • 期刊:
  • 影响因子:
    4
  • 作者:
    Cardon, Zoe G.;Peredo, Elena L.;Bezanilla, Magdalena
  • 通讯作者:
    Bezanilla, Magdalena
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Zoe Cardon其他文献

Zoe Cardon的其他文献

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{{ truncateString('Zoe Cardon', 18)}}的其他基金

Collaborative Research: MSB: The Role of Sulfur Oxidizing Bacteria in Salt Marsh C and N Cycling
合作研究:MSB:硫氧化细菌在盐沼碳氮循环中的作用
  • 批准号:
    1050713
  • 财政年份:
    2011
  • 资助金额:
    $ 63万
  • 项目类别:
    Continuing Grant
Desert Microbial Activity in the Rhizosphere Oasis
根际绿洲的沙漠微生物活动
  • 批准号:
    0415938
  • 财政年份:
    2004
  • 资助金额:
    $ 63万
  • 项目类别:
    Standard Grant
Dissertation Research: Does Hydraulic Redistribution Increase Microbial Activity in the Rhizosphere?
论文研究:水力重新分配是否会增加根际微生物活动?
  • 批准号:
    0408066
  • 财政年份:
    2004
  • 资助金额:
    $ 63万
  • 项目类别:
    Standard Grant
SGER: Developing a New Miniaturized Sensor for Detecting Glucose in Soil
SGER:开发一种用于检测土壤中葡萄糖的新型微型传感器
  • 批准号:
    0221620
  • 财政年份:
    2002
  • 资助金额:
    $ 63万
  • 项目类别:
    Standard Grant
Analytical and Quantitative Understanding of Integrative Plant Biology through Coursework and Independent Student Research
通过课程作业和学生独立研究对综合植物生物学的分析和定量理解
  • 批准号:
    9751268
  • 财政年份:
    1997
  • 资助金额:
    $ 63万
  • 项目类别:
    Standard Grant
Analytical and Quantitative Understanding of Integrative Plant Biology through Coursework and Independent Student Research
通过课程作业和学生独立研究对综合植物生物学的分析和定量理解
  • 批准号:
    9796271
  • 财政年份:
    1997
  • 资助金额:
    $ 63万
  • 项目类别:
    Standard Grant

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  • 批准号:
    2326816
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    2024
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Postdoctoral Fellowship: STEMEdIPRF: Towards a Diverse Professoriate: Experiences that Inform Underrepresented Scholars' Perceptions of Value Alignment and Career Decisions
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    2414042
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