The role of the plant-specific DNA-interacting 3xHMG-box proteins in mitosis (and meiosis)

植物特异性 DNA 相互作用 3xHMG-box 蛋白在有丝分裂（和减数分裂）中的作用

• 批准号: 214142277
• 负责人: Professor Dr. Klaus Grasser
• 金额: --
• 依托单位: Lehrstuhl für Zellbiologie und Pflanzenbiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/214142277?language=en
• 关键词: role; plant; specific; DNA; interacting

A characteristic of cell division is the remarkable condensation of the genomic DNA into mitotic (or meiotic) chromosomes. The compaction of the chromosomes is of fundamental importance for faithful chromosome segregation during mitosis and meiosis, and therefore for proper inheritance. In preparatory work, we have started studying a so far uncharacterised family of plant-specific proteins, containing three HMG-box DNAbinding domains, termed 3xHMG-box proteins. Two Arabidopsis 3xHMG-box proteins interact with DNA, but unlike other HMG-box proteins they display cell cycle dependent expression, peaking in cells undergoing mitosis (and meiosis). Both the Arabidopsis 3xHMG-box proteins associate with condensed mitotic/meiotic chromosomes and 3xHMG-box2 generally associates with condensed chromosomes, whereas 3xHMG-box1 displays a clear preference for 45S rDNA loci. Currently, it is unclear which role these proteins play during mitosis/meiosis, and therefore we intend to address this question in this proposed project. Plants with altered expression levels of the two 3xHMG-box proteins will be analysed for possible phenotypic differences (relative to control plants), for effects on chromosome structure/segregation, histone modifications, 45S rDNA activity and cell cycle abnormalities. We also analyse the molecular differences between the two 3xHMG-box proteins and identify proteins interacting with the 3xHMG-box proteins to learn more about their cellular function. In summary, this project will provide insight into the biological role of the 3xHMG-box proteins in cell division.

细胞分裂的一个特征是基因组DNA显著凝聚成有丝分裂(或减数分裂)染色体。在有丝分裂和减数分裂过程中，染色体的紧凑对染色体的正确分离是至关重要的，因此对适当的遗传也是非常重要的。在准备工作中，我们已经开始研究到目前为止还没有特征的植物特异蛋白家族，包含三个HMG-box DNA结合结构域，称为3xHMG-box蛋白。两个拟南芥3xHMG-box蛋白与DNA相互作用，但与其他HMG-box蛋白不同，它们表现出细胞周期依赖性的表达，在经历有丝分裂(和减数分裂)的细胞中达到顶峰。拟南芥3xHMG-box蛋白与浓缩有丝分裂/减数分裂染色体有关，3xHMG-box2蛋白与浓缩染色体有关，而3xHMG-box1蛋白对45S rDNA有明显的偏好。目前，这些蛋白在有丝分裂/减数分裂中起什么作用还不清楚，因此我们打算在这个拟议的项目中解决这个问题。将分析这两种3xHMG-box蛋白表达水平改变的植物可能存在的表型差异(相对于对照植物)、对染色体结构/分离、组蛋白修饰、45S rDNA活性和细胞周期异常的影响。我们还分析了两个3xHMG-box蛋白之间的分子差异，并鉴定了与3xHMG-box蛋白相互作用的蛋白质，以了解更多关于它们的细胞功能。综上所述，该项目将深入了解3xHMG-box蛋白在细胞分裂中的生物学作用。