Indirect MFM for sensing magnetic nanoparticles

建议：1403574PI：Agarwal，Gunjan标题：用于传感磁性纳米颗粒的间接MFM铁可以以纳米颗粒的形式存在于人体内。此外，磁性纳米颗粒还广泛应用于许多生物领域。准确检测磁性纳米颗粒的生物传感技术还不是很成熟。该项目将开发一种新的基于显微镜的技术来检测磁性纳米颗粒。这种发展可以帮助在多种病理和生物纳米技术应用中更准确地测定人体组织中的铁含量。各种具有独特和多功能性质的超顺磁性纳米颗粒被用于生物传感应用。体内发现的纳米级铁沉积在自然界中也是超顺磁性的。在生物系统中在单个粒子水平上表征这些纳米级磁性(铁)粒子的技术还不是很成熟。这项提议的总体目标是开发一种新的生物传感模式，即间接磁力显微镜(ID-MFM)，以检测纳米级的磁性颗粒。ID-MFM有望提供非常高的灵敏度和空间分辨率，超过现有生物传感器和成像模式的能力。这种独特的方法可以用来测定少量样品中的磁性(铁)含量，并有望在评估生物液、细胞和组织切片方面找到广泛的应用。由于铁蛋白在生物医学和生物纳米技术中的广泛应用，铁蛋白将被作为一种模型磁性纳米粒子进行研究。ID-MFM将被开发用于在体外、环境空气和流体环境中检测铁蛋白。拟议的工作还包括通过实践讲习班、研究经验和机构间合作，为K-8学生、本科生和研究生提供多层次的纳米技术丰富计划。

Proposal: 1403574PI: Agarwal, GunjanTitle: Indirect MFM for sensing magnetic nanoparticlesIron can exist in the human body in the form of nanoparticles. In addition, magnetic nanoparticles are widely used in a number of biological applications. Biosensing techniques to accurately detect magnetic nanoparticles are not well-developed. This project will develop a new microscopy-based technique to detect magnetic nanoparticles. The proposed development can help a more accurate determination of iron content in human tissue for multiple pathologies and in bio-nanotechnology applications.A variety of superparamagnetic nanoparticles with unique and multi-functional properties are utilized in biosensing applications. Nanoscale iron-deposits found in-vivo are also superparamagnetic in nature. Techniques to characterize these nanoscale magnetic (iron) particles in biological systems at the single particle level are not well-developed. The overall goal of this proposal is to develop a novel biosensing modality, namely, InDirect Magnetic Force Microscopy (ID-MFM), to detect nanoscale magnetic particles. ID-MFM is expected to offer very high sensitivity and spatial resolution beyond the capabilities of existing biosensors and imaging modalities. This unique approach can be used to determine magnetic (iron) content in small quantities of samples and is expected to find a wide variety of applications in evaluating biological fluids, cells and tissue sections. The iron-encapsulating protein, ferritin, will be studied as a model magnetic nanoparticle because of its versatile applications in biomedicine and bio-nanotechnology. ID-MFM will be developed to detect ferritin in-vitro, in ambient air and in a fluid environment. The proposed efforts also include a multi-level enrichment plan in nanotechnology for K-8 students, undergraduates and graduate students through a hands-on workshop, research experience and inter-institutional collaboration.