Indirect MFM for sensing magnetic nanoparticles

用于传感磁性纳米颗粒的间接 MFM

• 批准号: 1403574
• 负责人: Gunjan Agarwal
• 金额: $ 30万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2020-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1403574&HistoricalAwards=false
• 关键词: Indirect; MFM; sensing; magnetic; nanoparticles

Proposal: 1403574PI: Agarwal, GunjanTitle: Indirect MFM for sensing magnetic nanoparticlesIron can exist in the human body in the form of nanoparticles. In addition, magnetic nanoparticles are widely used in a number of biological applications. Biosensing techniques to accurately detect magnetic nanoparticles are not well-developed. This project will develop a new microscopy-based technique to detect magnetic nanoparticles. The proposed development can help a more accurate determination of iron content in human tissue for multiple pathologies and in bio-nanotechnology applications.A variety of superparamagnetic nanoparticles with unique and multi-functional properties are utilized in biosensing applications. Nanoscale iron-deposits found in-vivo are also superparamagnetic in nature. Techniques to characterize these nanoscale magnetic (iron) particles in biological systems at the single particle level are not well-developed. The overall goal of this proposal is to develop a novel biosensing modality, namely, InDirect Magnetic Force Microscopy (ID-MFM), to detect nanoscale magnetic particles. ID-MFM is expected to offer very high sensitivity and spatial resolution beyond the capabilities of existing biosensors and imaging modalities. This unique approach can be used to determine magnetic (iron) content in small quantities of samples and is expected to find a wide variety of applications in evaluating biological fluids, cells and tissue sections. The iron-encapsulating protein, ferritin, will be studied as a model magnetic nanoparticle because of its versatile applications in biomedicine and bio-nanotechnology. ID-MFM will be developed to detect ferritin in-vitro, in ambient air and in a fluid environment. The proposed efforts also include a multi-level enrichment plan in nanotechnology for K-8 students, undergraduates and graduate students through a hands-on workshop, research experience and inter-institutional collaboration.

提案：1403574 PI：Agarwal，GunjanTitle：磁性纳米粒子的间接磁流体力学传感铁可以纳米粒子的形式存在于人体内。此外，磁性纳米颗粒广泛用于许多生物应用中。准确检测磁性纳米颗粒的生物传感技术还没有得到很好的发展。该项目将开发一种新的基于显微镜的技术来检测磁性纳米颗粒。该方法可以更准确地测定人体组织中的铁含量，用于多种病理和生物纳米技术应用。在体内发现的纳米级铁沉积物在性质上也是超顺磁性的。在单颗粒水平上表征生物系统中这些纳米级磁性（铁）颗粒的技术还没有得到很好的发展。本提案的总体目标是开发一种新的生物传感模式，即间接磁力显微镜（ID-MFM），以检测纳米级磁性颗粒。ID-MFM有望提供超出现有生物传感器和成像模式能力的非常高的灵敏度和空间分辨率。这种独特的方法可用于测定少量样品中的磁性（铁）含量，并有望在评估生物液体，细胞和组织切片中找到各种各样的应用。由于铁蛋白在生物医学和生物纳米技术中的广泛应用，将作为磁性纳米颗粒的模型进行研究。ID-MFM将被开发用于在体外、环境空气和流体环境中检测铁蛋白。拟议的努力还包括通过实践讲习班、研究经验和机构间合作，为K-8学生、本科生和研究生提供纳米技术方面的多层次丰富计划。

项目成果

Indirect magnetic force microscopy

间接磁力显微镜

• DOI: 10.1039/c9na00193j
• 发表时间: 2019
• 期刊: Nanoscale Advances
• 影响因子: 4.7
• 作者: Sifford, Joshua;Walsh, Kevin J.;Tong, Sheng;Bao, Gang;Agarwal, Gunjan
• 通讯作者: Agarwal, Gunjan

Ferritin Mineral Core Composition in Health and Disease

健康和疾病中的铁蛋白矿物质核心成分

• DOI: 10.1017/s1431927616006620
• 发表时间: 2016
• 期刊: Microscopy and Microanalysis
• 影响因子: 2.8
• 作者: Blissett, Angela;Ollander, Brooke;Deng, Binbin;Nocera, Tanya;Calomeni, Edward;Yin, Feng;McComb, David;McTigue, Dana;Agarwal, Gunjan
• 通讯作者: Agarwal, Gunjan

Distinguishing ferritin from apoferritin using magnetic force microscopy

使用磁力显微镜区分铁蛋白和脱铁铁蛋白

• DOI: 10.1088/0957-4484/25/46/461001
• 发表时间: 2014
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Nocera, Tanya M;Zeng, Yuzhi;Agarwal, Gunjan
• 通讯作者: Agarwal, Gunjan