EAPSI: Investigating the production of stress-induced proteins following nitrite exposure in the commonly farmed shrimp Litopenaeus vannamei

EAPSI：调查常见养殖的凡纳滨对虾暴露于亚硝酸盐后应激诱导的蛋白质的产生

• 批准号: 1414830
• 负责人: Jason Wang
• 金额: $ 0.51万
• 依托单位: Wang Jason
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1414830&HistoricalAwards=false
• 关键词: EAPSI; Investigating; production; stress; induced

Farmed shrimp comprises a significant portion of global food production, and most of shrimp production comes from Pacific countries such as Taiwan. Aquaculture facilities commonly stock shrimp in unnaturally high densities and produce excessive organic wastes. Shrimp excrete ammonia waste, which is converted to nitrite by microbes. Nitrite accumulates inside the shrimp, causing damage to proteins and cells. In many animals, a common response to stress is the production of a class of proteins called heat shock proteins (HSP). These proteins preserve the shape and function of damaged proteins. However, little is understood about the role of HSPs in aquacultured shrimp. This study aims to quantify the production of HSPs in several shrimp tissues following nitrite stress exposures. This study will be conducted in Taiwan in collaboration with Dr. Cheng-Hao Tang (Institute of Marine Biotechnology, National Dong Hwa University), an expert in stress responses of aquacultured animals. Understanding how HSPs alleviate stress in shrimp will elucidate the effects that aquaculture conditions have on commercially important organisms and their surrounding environments.Nitrite is an important stressor because it is a common byproduct of microbial metabolism in marine systems, and also a reactive oxidative species. In aquaculture ponds, nitrite can reach an excess of 20 mg mL-1, easily entering the shrimp's system through the gills. This study will quantify production of four different HSP mRNA transcripts and proteins using quantitative PCR and Western Blots. Quantification of HSP transcripts and proteins will be done in the gills and the hepatopancreas where enzymes are produced. Nitrite is one of many stressors that may induce HSP production, and this work will further the framework for examining diverse stress responses using HSPs. The NSF EAPSI award is funded in collaboration with the National Science Council of Taiwan.

养殖虾占全球粮食生产的很大一部分，大部分虾生产来自台湾等太平洋国家。水产养殖设施通常在非自然的高密度中储存虾，并产生过多的有机废物。虾排泄的氨废物被微生物转化为亚硝酸盐。亚硝酸盐积聚在虾内，从而损害了蛋白质和细胞。在许多动物中，对压力的常见反应是产生一种称为热休克蛋白（HSP）的蛋白质。这些蛋白质保留受损蛋白质的形状和功能。但是，关于HSP在水产养殖虾中的作用几乎没有理解。这项研究旨在量化亚硝酸盐应力暴露后几种虾组织中HSP的产生。这项研究将在台湾与Cheng-Hao Tang博士（国立董家大学海洋生物技术研究所）合作，这是水产养殖动物的压力反应专家。了解HSP如何减轻虾中的压力将阐明水产养殖条件对商业重要生物及其周围环境的影响。硝酸盐是重要的压力源，因为它是海洋系统中微生物代谢的共同副产品，也是一种反应性氧化物种。在水产养殖池塘中，亚硝酸盐可以达到20 mg ML-1，很容易通过g进入虾的系统。这项研究将使用定量PCR和Western印迹来量化四个不同的HSP mRNA转录本和蛋白质的产生。 HSP转录本和蛋白质的定量将在产生酶的g和肝癌中进行。亚硝酸盐是可能引起HSP产生的众多压力源之一，这项工作将进一步为使用HSPS检查多样化的压力反应提供框架。 NSF EAPSI奖与台湾国家科学委员会合作。