EAPSI: Investigating the production of stress-induced proteins following nitrite exposure in the commonly farmed shrimp Litopenaeus vannamei

EAPSI：调查常见养殖的凡纳滨对虾暴露于亚硝酸盐后应激诱导的蛋白质的产生

• 批准号: 1414830
• 负责人: Jason Wang
• 金额: $ 0.51万
• 依托单位: Wang Jason
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1414830&HistoricalAwards=false
• 关键词: EAPSI; Investigating; production; stress; induced

Farmed shrimp comprises a significant portion of global food production, and most of shrimp production comes from Pacific countries such as Taiwan. Aquaculture facilities commonly stock shrimp in unnaturally high densities and produce excessive organic wastes. Shrimp excrete ammonia waste, which is converted to nitrite by microbes. Nitrite accumulates inside the shrimp, causing damage to proteins and cells. In many animals, a common response to stress is the production of a class of proteins called heat shock proteins (HSP). These proteins preserve the shape and function of damaged proteins. However, little is understood about the role of HSPs in aquacultured shrimp. This study aims to quantify the production of HSPs in several shrimp tissues following nitrite stress exposures. This study will be conducted in Taiwan in collaboration with Dr. Cheng-Hao Tang (Institute of Marine Biotechnology, National Dong Hwa University), an expert in stress responses of aquacultured animals. Understanding how HSPs alleviate stress in shrimp will elucidate the effects that aquaculture conditions have on commercially important organisms and their surrounding environments.Nitrite is an important stressor because it is a common byproduct of microbial metabolism in marine systems, and also a reactive oxidative species. In aquaculture ponds, nitrite can reach an excess of 20 mg mL-1, easily entering the shrimp's system through the gills. This study will quantify production of four different HSP mRNA transcripts and proteins using quantitative PCR and Western Blots. Quantification of HSP transcripts and proteins will be done in the gills and the hepatopancreas where enzymes are produced. Nitrite is one of many stressors that may induce HSP production, and this work will further the framework for examining diverse stress responses using HSPs. The NSF EAPSI award is funded in collaboration with the National Science Council of Taiwan.

水产养殖设施通常以不自然的高密度饲养虾，并产生过多的有机废物。虾排出的氨废物被微生物转化为亚硝酸盐。亚硝酸盐在虾体内积累，对蛋白质和细胞造成损害。在许多动物中，对压力的一种常见反应是产生一类称为热休克蛋白（HSP）的蛋白质。这些蛋白质保持受损蛋白质的形状和功能。然而，人们对热休克蛋白在水产养殖对虾中的作用知之甚少。本研究旨在量化亚硝酸盐胁迫暴露后几种虾组织中热休克蛋白的产生。本研究将在台湾进行，并与水产养殖动物应激反应专家汤成浩博士（国立东华大学海洋生物技术研究所）合作。了解热休克蛋白如何减轻对虾的应激将阐明水产养殖条件对商业上重要的生物及其周围环境的影响。亚硝酸盐是海洋微生物代谢过程中常见的副产物，也是一种活性氧化物质，是一种重要的应激源。在水产养殖池塘中，亚硝酸盐可超过20 mg mL-1，很容易通过鳃进入对虾的系统。本研究将使用定量PCR和Western Blots对四种不同的HSP mRNA转录物和蛋白的产生进行量化。热休克蛋白转录本和蛋白质的定量将在产生酶的鳃和肝胰腺中进行。亚硝酸盐是可能诱导热休克蛋白产生的众多应激源之一，这项工作将进一步研究使用热休克蛋白的各种应激反应的框架。​