EAPSI: Assessing Microbial Activity in the Shale and Implications for Natural Gas Extraction

EAPSI：评估页岩中的微生物活动及其对天然气开采的影响

• 批准号: 1414988
• 负责人: Jessie Wishart
• 金额: $ 0.51万
• 依托单位: Wishart Jessie R
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1414988&HistoricalAwards=false
• 关键词: EAPSI; Assessing; Microbial; Activity; Shale

Extracting natural gas from shales by hydrofracturing has increased globally in recent years. Hydrofracturing is a modified version of oil well drilling that is designed for extraction gases rather than fluids from a subsurface reservoir. Hydrofracturing produces millions of liters of contaminated waste fluid that is difficult to dispose of in an economical and environmentally-friendly manner. Treatment of these fluids is a great public health concern. The origin of contaminants in waste fluids is not well understood nor is the role of microorganisms that can remain biologically active in the fluids and shales. This research will investigate the degree to which microbial cells present on shales are active and the types of carbon sources that they utilize including whether these sources can come from the shale itself. The research will be conducted in collaboration with Drs. Fumio Inagaki and Yuki Morono, experts on subsurface microbial communities, at the Japan Agency for Marine-Earth Science and Technology which will provide access to a premier biogeochemistry laboratory. This research will develop a better understanding of biogeochemical processes occurring during shale hydrofracturing and should lead to improved waste fluid treatment and hydrofracturing methods to reduce environmental contamination. The breakdown of organic compounds in the subsurface by microbiological activity can result in the co-release of toxic elements such as heavy metals. This research will determine what organic compounds associated with drilling fluids and Marcellus shale are biologically available. Nano secondary ion mass spectrometry (NanoSIMS) will be used to trace the assimilation of stable isotope-labeled organic compounds into microbial cells derived from waste fluids as well as whether cells attached to shale can utilize native organic compounds in the shale. NanoSIMS analysis determines the percentage of cells that assimilate a certain stable isotope-labeled or shale-derived carbon source. This NSF EAPSI award is funded in collaboration with the Japan Society for the Promotion of Science.

近年来，通过水力压裂从页岩中提取天然气的做法在全球范围内有所增加。水力压裂是一种改进的油井钻井技术，旨在从地下储层中提取气体而不是流体。水力压裂会产生数百万升受污染的废液，这些废液很难以经济和环保的方式处理。这些液体的处理是一个重大的公共卫生问题。废液中污染物的来源尚不清楚，能够在废液和页岩中保持生物活性的微生物的作用也不清楚。这项研究将调查页岩中微生物细胞的活跃程度，以及它们利用的碳源类型，包括这些碳源是否来自页岩本身。该研究将与博士合作进行。稻垣文雄和Morono Yuki是日本海洋地球科学技术机构的地下微生物群落专家，该机构将提供一个一流的生物地球化学实验室。这项研究将有助于更好地理解页岩水力压裂过程中发生的生物地球化学过程，并有助于改进废液处理和水力压裂方法，以减少环境污染。微生物活动对地下有机化合物的分解可导致重金属等有毒元素的共同释放。这项研究将确定钻井液和Marcellus页岩中哪些有机化合物是生物可利用的。纳米二次离子质谱法（NanoSIMS）将用于追踪稳定同位素标记的有机化合物在废液衍生的微生物细胞中的同化作用，以及附着在页岩上的细胞是否可以利用页岩中的天然有机化合物。NanoSIMS分析确定了吸收某种稳定同位素标记或页岩衍生碳源的细胞百分比。NSF EAPSI奖是与日本科学促进会合作资助的。