EAPSI: Using Molecular Genomics to Analyze the Chloroplast Genome of Illicium

EAPSI：利用分子基因组学分析八角的叶绿体基因组

• 批准号: 1415008
• 负责人: Opal Leonard
• 金额: $ 0.51万
• 依托单位: Leonard Opal R
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1415008&HistoricalAwards=false
• 关键词: EAPSI; Using; Molecular; Genomics; Analyze

Illicium is a genus of flowering plants comprising roughly 40 species across the Southeast of United States (U.S.), Mexico, Greater Antilles, and Southeast Asia. Illicium is recognized by its star-shaped fruits, which give the group the common name "star anise". The fruits are found in teas, incense, and Indian and Chinese cuisine, and some species of Illicium are producers of an important precursor of Tamiflu. Illicium is one of the earliest diverging lineages of flowering plants and is therefore an important model for the study of floral development and evolution. In this study samples of Illicium that are inaccessible in the U.S. will be collected. DNA from the samples will be extracted, and the chloroplast genomes will be sequenced using next-generation sequencing. This research will be conducted in collaboration with Dr. Zhiduan Chen at the Institute of Botany at the Chinese Academy of Sciences, granting access to 30 species of Illicium found in Asia that are not accessible in the U.S. Previous molecular studies supported recognition of two major groups: one Old World, located in Asia, and one New World, located in southeast U.S. Species resolution within both groups is lacking, leaving questions regarding boundaries between species and biogeographic history unanswered. Molecular tools are being developed for use to address these questions and long standing taxonomic issues. This research will use the sequenced genomes for comparison to identify the most informative noncoding chloroplast regions in species of New and Old World Illicium, and use the most informative regions to analyze the evolutionary relationships within Illicium. The genome sequences will be deposited into GenBank. This NSF EAPSI award is funded in collaboration with the Chinese Ministry of Science and Technology.

八角属(Illicium)是一种开花植物，由大约40种植物组成，分布在美国东南部、墨西哥、大安的列斯群岛和东南亚。八角因其星形的果实而被人们所认识，这就是八角的俗称。这种水果存在于茶、香以及印度和中国的菜肴中，一些种类的八角是一种重要的达菲前体。八角是最早分化的开花植物谱系之一，因此是研究花发育和进化的重要模式。在这项研究中，将收集在美国无法获得的伊利西姆样本。将从样本中提取DNA，并使用下一代测序对叶绿体基因组进行测序。这项研究将与中国科学院植物研究所的陈志端博士合作进行，允许进入在亚洲发现的30种美国无法获得的八角属植物。以前的分子研究支持两个主要类群的识别：一个是位于亚洲的旧世界，另一个是位于美国东南部的新大陆。这两个类群中的物种缺乏分辨，物种和生物地理历史之间的界限问题没有得到回答。正在开发用于解决这些问题和长期存在的分类学问题的分子工具。本研究将利用测序后的基因组进行比较，以确定新旧大陆八角属植物中信息量最大的非编码叶绿体区域，并利用这些信息量最大的区域分析八角属植物的进化关系。基因组序列将被存入GenBank。该NSF EAPSI奖是与中国科技部合作资助的。