Natural Trap Cave Revisited: Ancient DNA, Climate and the Megafaunal Extinction

重温自然陷阱洞穴：古代 DNA、气候和巨型动物灭绝

• 批准号: 1425059
• 负责人: Julie Meachen
• 金额: $ 22.83万
• 依托单位: Des Moines University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1425059&HistoricalAwards=false
• 关键词: Natural; Trap; Cave; Revisited; Ancient

Non-technical abstractThe end of the ice age (about 11,000 years ago) was a time of radical change in North America. Many species of large mammals went extinct, along with their habitats. Climate played a major role in this extinction event and current climate change continues to affect and alter the world today. This project will analyze ancient DNA from ice age animals from Natural Trap Cave, Wyoming to examine how these past climate changes relate to genetic variability and extinction. This research will lead to a better overall understanding of how climate change is related to population structure, changes in genetic variation, and ultimately extinction, which may have potential effects on how biologists think about and mitigate the current human-caused climate change occurring today. This project will also provide many opportunities for scientific engagement and outreach to both the general public and in classroom settings. The intriguing nature of the excavations and ultimate findings make this expedition a perfect opportunity to bring science to the general public through print and television media. Additionally, this is an excellent opportunity to give high school students real time experience on how field science is performed. Via remote participation on the web, this project will give students the opportunity to participate in the experience of what it is like to excavate a fossil site, live out in the field, and unearth bones of gigantic, long-dead beasts, but in a safe, virtual setting.Technical abstractThe end-Pleistocene was a time of radical change in North America. Many species of large mammals went extinct, along with the loss of a variety of associated ecosystems. Climate played a major role in this extinction event and current climate change continues to affect and alter ecosystems today. This project will re-open Natural Trap Cave in northern Wyoming to investigate paleoecological responses to climate change and how this relates to the megafaunal extinction events at the end of the Pleistocene. The permanently cold and moist conditions at NTC have preserved a large collection of megafaunal bones which still contain ancient DNA (aDNA) and collagen molecules. This rare resource is optimal to perform population-level analyses of genetic variation in the critical phases before, during, and after the end-Pleistocene extinction events. Key questions investigated in this project include: 1) Was the Pleistocene extinction event preceded by, or concurrent with a large loss of genetic diversity? 2) Are morphological changes at the Pleistocene/Holocene boundary the product of adaptation, or immigration? 3) Are major shifts in morphological and genetic variation correlated with climate change? Using aDNA, AMS radiocarbon dates, stable isotope analyses, morphological analyses, and previous data from NTC this project will investigate changes in climatic conditions and how they correlate with genetic diversity and morphological change. This research will lead to a better understanding of how climate change drives extinction and may reveal correlations between climate change, metapopulation structure and changes in genetic and morphological variation.

冰河时代的结束（大约11,000年前）是北美发生根本变化的时期。许多种类的大型哺乳动物沿着灭绝，它们的栖息地也随之消失。气候在这次灭绝事件中发挥了重要作用，目前的气候变化继续影响和改变当今世界。该项目将分析来自怀俄明州自然陷阱洞穴的冰河时代动物的古老DNA，以研究这些过去的气候变化如何与遗传变异和灭绝有关。 这项研究将使人们更好地全面了解气候变化如何与人口结构、遗传变异的变化以及最终的灭绝有关，这可能会对生物学家如何思考和缓解目前人类造成的气候变化产生潜在影响。该项目还将提供许多机会，使科学参与和推广到公众和课堂环境中。挖掘和最终发现的有趣性质使这次探险成为通过印刷和电视媒体将科学带给公众的绝佳机会。此外，这是一个极好的机会，让高中生真实的时间经验，对如何执行领域科学。通过网络上的远程参与，这个项目将让学生有机会参与挖掘化石遗址的体验，在野外生活，挖掘巨大的，长期死亡的野兽的骨头，但在一个安全的，虚拟的环境中。技术摘要更新世末期是北美发生根本变化的时期。许多大型哺乳动物物种灭绝，沿着丧失了各种相关的生态系统。气候在这次灭绝事件中发挥了重要作用，目前的气候变化继续影响和改变今天的生态系统。该项目将重新开放怀俄明州北方的自然陷阱洞穴，以调查古生态对气候变化的反应，以及这与更新世末巨型动物灭绝事件的关系。NTC永久寒冷和潮湿的条件保存了大量的巨型动物骨骼，其中仍然含有古老的DNA（aDNA）和胶原蛋白分子。这种罕见的资源是最佳的执行人口水平的遗传变异分析的关键阶段之前，期间和之后的更新世末灭绝事件。该项目研究的关键问题包括：1）更新世灭绝事件之前，或同时与遗传多样性的大量损失？2)更新世/全新世边界的形态变化是适应的产物还是移民的产物？3)形态和遗传变异的主要变化与气候变化相关吗？利用aDNA、AMS放射性碳测年、稳定同位素分析、形态分析和NTC先前的数据，该项目将调查气候条件的变化以及它们与遗传多样性和形态变化的关系。这项研究将有助于更好地了解气候变化如何推动物种灭绝，并可能揭示气候变化、集合种群结构与遗传和形态变异变化之间的相关性。

项目成果

Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes

• DOI: 10.1371/journal.pone.0209499
• 发表时间: 2019-02
• 期刊: PLoS ONE
• 影响因子: 3.7
• 作者: Stephen M. Richards;N. Hovhannisyan;Matthew Gilliham;Joshua Ingram;B. Skadhauge;Holly Heiniger;B. Llamas

Locomotor correlates of the scapholunar of living and extinct carnivorans

• DOI: 10.1002/jmor.21024
• 发表时间: 2019-08-01
• 期刊: JOURNAL OF MORPHOLOGY
• 影响因子: 1.5
• 作者: Dunn, Rachel H.;Cooper, Candice;Meachen, Julie A.
• 通讯作者: Meachen, Julie A.

Dire wolves were the last of an ancient New World canid lineage

• DOI: 10.1038/s41586-020-03082-x
• 发表时间: 2021-01
• 期刊: Nature
• 影响因子: 64.8
• 作者: Angela R. Perri;Kieren J. Mitchell;A. Mouton;Sandra Álvarez-Carretero;A. Hulme-Beaman;J. Haile;A. Jamieson;J. Meachen;Audrey T. Lin;B. Schubert;C. Ameen;E. Antipina;P. Bover;Selina Brace;Alberto Carmagnini;Christian Carøe;J. A. Samaniego Castruita;J. Chatters;K. Dobney;Mario dos Reis;A. Evin;P. Gaubert;Shyam Gopalakrishnan;Graham Gower;Holly Heiniger;K. Helgen;J. Kapp;P. Kosintsev;Anna Linderholm;A. Ozga;Samantha Presslee;Alexander T. Salis;Nedda F. Saremi;Colin J. Shew;K. Skerry;Dmitry E Taranenko;M. Thompson;M. Sablin;Y. Kuzmin;M. Collins;Mikkel-Holger S. Sinding;M. Gilbert;A. Stone;B. Shapiro;B. Van Valkenburgh;R. Wayne;G. Larson;A. Cooper;L. Frantz

Postcranial diversity and recent ecomorphic impoverishment of North American gray wolves

北美灰狼的颅后多样性和近期生态形态贫化

• DOI: 10.1098/rsbl.2017.0613
• 发表时间: 2018
• 期刊: Biology Letters
• 影响因子: 3.3
• 作者: Tomiya, Susumu;Meachen, Julie A.
• 通讯作者: Meachen, Julie A.