MRI: Acquisition of an Infrared (IR) Fluorescence Imager

MRI：获取红外 (IR) 荧光成像仪

• 批准号: 1428384
• 负责人: Joshua Sandquist
• 金额: $ 6.18万
• 依托单位: Grinnell College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1428384&HistoricalAwards=false
• 关键词: MRI; Acquisition; Infrared; IR; Fluorescence

Non Technical Abstract:The LI-COR Odyssey Infrared Imaging System acquired through this award will enable highly quantitative investigations in diverse areas of research such as mitotic spindle function, muscle function, obesity, receptor function, and plant responses to heat stress. This instrument uses infrared (IR) technology for the highly sensitive and accurate measurement of biological molecules over several orders of magnitude, thus facilitating the detection of very low but biologically meaningful signals in a diverse group of applications, including protein and nucleic acid gels, protein arrays, In-Cell Westerns and tissue sections. Further, this instrumentation will be incorporated into undergraduate science education and research training programs in biology, biochemistry, neuroscience, and psychology.Technical Abstract:This highly sensitive imaging system will be used in a broad range of ongoing research projects, making previously qualitative assays quantitative. In particular, the Odyssey imaging system will be used to: a) examine the role of the protein myosin-10 in mitotic spindle function by facilitating dominant-negative analysis; b) investigate the involvement of high-fat-diet-induced changes in levels of the adipose hormone leptin in the hippocampus; c) analyze the contribution of the 3' untranslated region of the Rubisco activase gene to the stability of the Rubisco activase protein and thus to the heat stress response in plants; d) explore the role of perisynaptic glial cells at the neuromuscular junction by determining whether they express COX-2 in response to stimulation of their muscarinic acetylcholine receptors; and e) monitor the isolation and purification of neuronal nicotinic acetylcholine receptor subunits for use in studies to analyze drug-receptor interactions.

非技术摘要：通过该奖项获得的LI-COR Odyssey红外成像系统将在不同的研究领域进行高度定量的研究，如有丝分裂纺锤体功能，肌肉功能，肥胖，受体功能和植物对热应激的反应。该仪器使用红外（IR）技术对几个数量级的生物分子进行高灵敏度和准确的测量，从而有助于在各种应用中检测非常低但具有生物学意义的信号，包括蛋白质和核酸凝胶、蛋白质阵列、In-Cell Westerns和组织切片。此外，这种仪器将被纳入本科科学教育和研究培训计划，在生物学，生物化学，神经科学和psychology.Technical摘要：这种高灵敏度的成像系统将被用于广泛的正在进行的研究项目，使以前的定性分析定量。特别地，Odyssey成像系统将用于：a）通过促进显性负分析来检查蛋白质肌球蛋白-10在有丝分裂纺锤体功能中的作用; B）研究高脂肪饮食诱导的海马体中脂肪激素瘦素水平变化的参与; c）分析Rubisco活化酶基因的3'非翻译区对Rubisco活化酶蛋白的稳定性的贡献，从而对植物中的热胁迫响应的贡献; d）通过确定突触周围神经胶质细胞是否响应于其毒蕈碱乙酰胆碱受体的刺激而表达考克斯-2来探索它们在神经肌肉接头处的作用;和e）监测神经元烟碱乙酰胆碱受体亚单位的分离和纯化，以用于分析药物-受体相互作用的研究。