MRI: Acquisition of an automatic NMR sample changer

MRI：购买自动 NMR 样品更换器

• 批准号: 1429353
• 负责人: Arezue Boroujerdi
• 金额: $ 5.03万
• 依托单位: Claflin University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1429353&HistoricalAwards=false
• 关键词: MRI; Acquisition; automatic; NMR; sample

An award is made to Claflin University for the purchase of an automated nuclear magnetic resonance (NMR) sample changer to do NMR-based metabolomics research. The field of metabolomics focuses on changes to the small molecules that make up metabolism upon some stressful event or condition. NMR serves as an excellent detector of these small compounds; and statistical analysis is applied to many NMR sample replicates to increase the robustness of the metabolomics technique. The addition of an automated sample changer to Claflin?s existing 700 MHz NMR not only will make metabolomics research more efficient, but also the NMR will be more user-friendly. Specifically, the SampleCaseTM is mounted to the instrument at user height and eliminates the use of a ladder. Undergraduate students will gain more access to collecting NMR data and be trained in cutting-edge NMR-based metabolomics research. Claflin University has the third highest field NMR laboratory in the state of South Carolina, just behind the Medical University of South Carolina (MUSC) and the Hollings Marine Laboratory (HML). Although a predominately undergraduate university and HBCU (Historically Black Colleges and Universities), Claflin's NMR facility is becoming a state and nation wide shared facility with six collaborative metabolomics projects and plans to secure major users of the instrument from other future collaborative partners. The automated sample changer will enhance the current instrument capabilities, and is in line with the chemistry department's mission to not only become a state level shared NMR facility, but to support and enhance undergraduate research participation. With the addition of an automatic sample changer, Claflin's NMR facility will be more attractive to outside users as well as be able to accommodate multiple undergraduate metabolomics research projects. Housed in the Molecular Science and Research Center (MSRC) on Claflin?s campus, the extraordinary collection of state of the art instrumentation, including the 700 MHz Bruker NMR spectrometer, is unmatched by any other HBCU. PI Boroujerdi's laboratory specializes in NMR-based metabolomics research; however, with such a high field magnet, the capability to collect and analyze large bio-molecular samples via NMR is possible and open to any external users.Claflin undergraduates love NMR-based metabolomics research. PI Boroujerdi is the newest biochemistry faculty member at Claflin, and since the beginning of her tenure three years ago, her metabolomics group has grown exponentially (currently with to 14 undergraduate students, 4 graduate students, and 1 staff). The nature of metabolomics lends itself to collaborations which is ideal for Claflin undergraduates whose research interest are spread far and wide in basic biology research. With 6 collaborators across the nation, and the recent interest in metabolomics research at Claflin, the demand for the 700 MHz NMR has dramatically increased to the point of saturation. With automatic sample collection, the large number of routine one dimensional 1H spectra can be collected more efficiently, and the extremely inefficient personnel time wasted on manual sample insertion and data collection will be refocused on bench work and data analysis, further increasing the quality and quantity of data produced by the metabolomics group. In order to accommodate the increasing number of undergraduates participating in metabolomics research, which includes the requirement for science majors to write and defend a senior thesis prior to graduation, many projects and collaborators have been established, which translates into a large number of NMR samples and a need for more efficient NMR data collection. Sample preparation, interpretation of results, manuscript writing, and presenting results will be increased with the addition of an automated sample changer. Instead of devoting personnel time to manual routine NMR data collection, while data is collected in automation, PI Boroujerdi can devote her time to training undergraduates in metabolite extraction techniques, statistical analysis of NMR data, and combing the literature to understand the biological implications of the metabolomics results.

克拉夫林大学购买了一台自动核磁共振（NMR）样品转换器，用于进行基于NMR的代谢组学研究。代谢组学领域的重点是在一些压力事件或条件下构成代谢的小分子的变化。NMR作为这些小化合物的一个很好的检测器;统计分析应用于许多NMR样品重复，以增加代谢组学技术的鲁棒性。在克拉夫林中增加自动进样器？现有的700 MHz核磁共振不仅将使代谢组学研究更有效率，而且核磁共振将更加用户友好。具体来说，SampleCaseTM安装在仪器上，与用户高度相同，无需使用梯子。本科生将获得更多的机会收集NMR数据，并在尖端NMR为基础的代谢组学研究的培训。克拉夫林大学拥有南卡罗来纳州第三高的核磁共振实验室，仅次于南卡罗来纳州医科大学（MUSC）和霍林斯海洋实验室（HML）。虽然占主导地位的本科大学和HBCU（历史上的黑人学院和大学），克拉夫林的核磁共振设施正在成为一个国家和全国范围内的共享设施与六个合作代谢组学项目，并计划从其他未来的合作伙伴，以确保仪器的主要用户。自动进样器将增强当前仪器的能力，并符合化学系的使命，不仅成为国家级共享NMR设施，但支持和提高本科生的研究参与。随着自动进样器的增加，克拉夫林的核磁共振设备将对外部用户更具吸引力，并能够适应多个本科代谢组学研究项目。位于克拉夫林的分子科学与研究中心（MSRC）？在HBCU的校园内，最先进的仪器设备，包括700 MHz布鲁克NMR光谱仪，是任何其他HBCU无法比拟的。PI Boroujerdi的实验室专门从事基于NMR的代谢组学研究;然而，有了这样一个高磁场磁铁，通过NMR收集和分析大型生物分子样品的能力是可能的，并对任何外部用户开放。克拉夫林本科生喜欢基于NMR的代谢组学研究。PI Boroujerdi是克拉夫林最新的生物化学教员，自从她三年前开始任职以来，她的代谢组学小组呈指数级增长（目前有14名本科生，4名研究生和1名工作人员）。代谢组学的性质有助于合作，这是理想的克拉夫林本科生的研究兴趣是广泛传播的基础生物学研究。在全国范围内有6个合作者，以及最近在克拉夫林对代谢组学研究的兴趣，对700 MHz NMR的需求急剧增加到饱和点。通过自动样品收集，可以更有效地收集大量的常规一维1H光谱，并且浪费在手动样品插入和数据收集上的极其低效的人员时间将重新集中在实验室工作和数据分析上，进一步提高代谢组学组产生的数据的质量和数量。为了适应越来越多的本科生参与代谢组学研究，其中包括要求科学专业的学生在毕业前撰写和答辩高级论文，已经建立了许多项目和合作者，这转化为大量的NMR样本和更有效的NMR数据收集的需要。通过添加自动进样器，将增加样品制备、结果解释、手稿撰写和结果展示。PI Boroujerdi可以将时间投入到代谢物提取技术，NMR数据的统计分析以及梳理文献以了解代谢组学结果的生物学意义方面的培训本科生中，而不是将人员时间投入到手动常规NMR数据收集中，同时自动收集数据。