The role of Drosophila melanogaster condensin I subunits during male meiosis

异五聚体凝聚素复合物参与有丝分裂和减数分裂染色体凝聚结构的建立和维持。虽然在脊椎动物中，两种凝聚素复合物，凝聚素I和凝聚素II，在有丝分裂和减数分裂中的作用已经被很好地确定，但在果蝇中，分叉的凝聚素II复合物不是有丝分裂增殖所必需的。然而，凝集素II特异性基因Cap-H2和Cap-D3的突变导致卵子发生、雄性减数分裂和精子发生过程中的缺陷。在男性减数分裂和精子发生过程中的凝聚素I特异性成分的可能作用还没有被调查。然而，编码凝聚素I特异性亚基的基因在睾丸中表达。因此，提出了通过RNAi或靶向蛋白水解切割来特异性去除睾丸中的缩合蛋白I功能并确定雄性减数分裂和/或精子染色质成熟期间可能的表型的实验。

The heteropentameric condensin complex has been shown to be involved in establishing and main-taining the condensed structure of mitotic and meiotic chromosomes. While in vertebrates the role of two condensin complexes, condensin I and condensin II, has been well established during both mito-sis and meiosis, the diverged condensin II complex in Drosophila is not required for mitotic prolifera-tion. However, mutations in the condensin II-specific genes Cap-H2 and Cap-D3 result in defects dur-ing oogenesis, male meiosis and spermatogenesis. Possible roles for the condensin I-specific components in male meiosis and during spermatogenesis have not been investigated. However, the genes encoding condensin I-specific subunits are expressed in testes. Thus, experiments are proposed to specifically remove condensin I function in testes via RNAi or targeted proteolytic cleavage and to determine possible phenotypes during male meiosis and/or sperm chromatin maturation.