Characterizing the Sensorimotor Transformation in Drosophila olfactory system

果蝇嗅觉系统感觉运动转化的表征

• 批准号: 10752470
• 负责人: Samuel Paura Wechsler
• 金额: $ 4.77万
• 依托单位: DREXEL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10752470
• 关键词: Affect; Anatomy; Animals; Behavior; Behavior assessment; Behavioral; Binding; Brain; Bypass; Complex; Computer Models; Data; Data Set; Databases; Dendrites; Drosophila genus; Drosophila melanogaster; Electrons; Electrophysiology (science); Environment; Excision; Future; Goals; Horns; Image; Individual; Knowledge; Laboratories; Lateral; Light; Lobe; Locomotion; Maps; Maxilla; Measures; Modeling; Motor; Movement; Mushroom Bodies; Nervous System; Neurons; Neurosciences; Odors; Olfactory Pathways; Olfactory Receptor Neurons; Outcome; Output; Pattern; Radial; Research; Role; Sensory; Signal Transduction; Stereotyping; Stimulus; Synapses; System; Techniques; Time; Whole-Cell Recordings; Work; behavior influence; behavior measurement; behavioral response; cell type; connectome; experience; fly; genetic approach; insight; light intensity; neural; neural circuit; neurotransmission; olfactory receptor; optogenetics; programs; receptor; response; sensory input; sensory integration

Characterizing the Sensorimotor Transformation in Drosophila olfactory system Understanding how the nervous system transforms sensory inputs into motor commands is a fundamental question in neuroscience. To understand how the nervous system performs these complex sensorimotor transformations we must be able to provide a well-controlled stimulus that elicits complex, multisequence behaviors and a means to quantitatively analyze this behavior, as well as have a complete knowledge of the underlying neural circuitry involved in this behavior. Here, we look to characterize the sensorimotor transformation occurring within two olfactory receptor neuron (ORN) classes in Drosophila melanogaster and identify how they impact behavior. Using an electron micrograph dataset of the fly brain we can identify the connection patterns between the first-order neurons of the olfactory system and the downstream second- and third-order neurons. The fly is highly tractable and we will use genetic strategies to optogenetically activate this first-order ORN classes and record from the second- and third- order neurons in response to this activation. By observing fly behavior in response to this ORN activation we can determine the relationship between its selective activation and its impact on behavior, as well as how the transformation between the first- to second- and second- to third- order neurons impact this expressed behavior. By combining optogenetics, electrophysiology, computational modeling, and behavior, this proposal seeks to further our understanding of how the nervous system integrates sensory information to execute an accompanying motor plan.

果蝇嗅觉系统感觉运动转换的特征 了解神经系统如何将感觉输入转化为运动指令是一个 神经科学的基本问题。为了了解神经系统如何执行这些功能， 复杂的感觉运动转换，我们必须能够提供一个良好控制的刺激， 这就解释了复杂的、多序列的行为，并提供了一种定量分析的方法， 行为，以及有一个完整的知识的基础神经回路参与 这种行为。在这里，我们希望描述发生在 两个嗅觉受体神经元（ORN）类在果蝇，并确定如何， 影响行为。利用苍蝇大脑的电子显微图像数据集，我们可以识别出 嗅觉系统的一阶神经元和下游神经元之间的连接模式 二级和三级神经元。苍蝇是非常听话的，我们将使用遗传策略， 光遗传学激活这种一阶ORN类，并记录从第二和第三- 神经元对这种激活的反应。通过观察苍蝇对这种ORN的反应， 激活，我们可以确定其选择性激活与其对 行为，以及如何转换之间的第一至第二和第二至第三， 顺序神经元影响这种表达的行为。通过结合光遗传学，电生理学， 计算建模和行为，这项建议旨在进一步了解我们如何 神经系统整合感觉信息以执行伴随的运动计划。