CSBR: Natural History: Development of a Liquid Nitrogen System for the Genetic Resources Collection, Natural Science Research Laboratory, Museum of Texas Tech University

CSBR：自然历史：用于遗传资源收集的液氮系统的开发，德克萨斯理工大学博物馆自然科学研究实验室

• 批准号: 1451925
• 负责人: Robert Bradley
• 金额: $ 41.2万
• 依托单位: Texas Tech University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1451925&HistoricalAwards=false
• 关键词: CSBR; Natural; History; Development; Liquid

An award is made to the Genetic Resources Collection (GRC) of the Natural Science Research Laboratory (NSRL), Museum of Texas Tech University to improve the security of its holdings through replacement of 16 mechanical -80oC freezers with a Liquid Nitrogen (-190oC) system. The samples housed at NSRL-GRC are used by researchers from around the world to address questions vital to science and society, such as advancing understanding of global biodiversity, the impact of humans on the natural world, and the transmission of disease between wildlife and humans. Each tissue sample is unique and irreplaceable, as it represents an individual at a specific point in time and space, and the samples are of unlimited actual and potential value to the science community in terms of on-going and future research. Outcomes of this project include: expanded storage capacity of the GRC, allowing for growth of the collection; education and training of graduate and undergraduate students in collection management practices; a public exhibit highlighting the significance of genetic resource collections to science and society; outreach materials for high school educators; development of a newsletter and a feature page on the NSRL website; refinement of curatorial standards; and reduced energy consumption by the transition to a green technology.The primary goal of this project is to replace the GRC's current storage system of 16 mechanical freezers with five vapor-phase liquid nitrogen (LN) freezers that preserve samples at -190°C. The lower temperature will ensure the integrity of the samples and preserve the entire spectrum of genomic data they contain, including molecules (RNA, viruses, bacteria, etc.) ensuring their potential to inform future research efforts. This will broaden the usage of the GRC beyond traditional disciplines, such as biodiversity and systematics, to include genomics and other developing fields of genetic, molecular, and environmental research. The project will also enable enhanced data accessibility by researchers and NSRL staff and allow for implementation of new curation procedures for GRC samples. This will be accomplished by: 1) annotating the NSRL database with genetic sequence database numbers and making those data available via data portals such as iDigBio and VertNet; 2) annotating the NSRL's internal database to indicate samples obtained from virus-positive vouchers and designate those samples with color-coded labels; and 3) identifying symbiotype and holotype genetic samples with color-coded labels. Overall, this project seeks to preserve the integrity and information content of the specimens while engaging the wider community by highlighting issues of relevance to their lives.

德克萨斯理工大学博物馆自然科学研究实验室（NSRL）的遗传资源收藏（GRC）获得了一项奖励，通过用液氮（-190℃）系统替换16台-80℃机械冷冻机来提高其馆藏的安全性。来自世界各地的研究人员使用NSRL-GRC的样本来解决对科学和社会至关重要的问题，例如促进对全球生物多样性的理解，人类对自然世界的影响，以及野生动物与人类之间疾病的传播。每个组织样本都是独一无二的，不可替代的，因为它代表了特定时间和空间点上的个体，对于科学界正在进行的和未来的研究来说，这些样本具有无限的实际和潜在价值。该项目的成果包括：扩大了GRC的存储容量，允许藏品的增长；对研究生和本科生进行催收管理实践的教育和培训；公开展览，突出遗传资源收藏对科学和社会的重要性；高中教育工作者外联材料；在国家科学研究实验室网站上编制通讯和专题页面；策展标准的细化；并通过向绿色技术过渡减少能源消耗。该项目的主要目标是用5个气相液氮（LN）冰柜取代GRC目前的16个机械冰柜的存储系统，这些冰柜可以在-190°C下保存样品。较低的温度将确保样品的完整性，并保存它们包含的整个基因组数据谱，包括分子（RNA，病毒，细菌等），确保它们为未来的研究工作提供信息的潜力。这将扩大GRC的应用范围，使其超越生物多样性和系统学等传统学科，包括基因组学和其他发展中的遗传、分子和环境研究领域。该项目还将增强研究人员和国家科学研究实验室工作人员对数据的可访问性，并允许对GRC样本实施新的管理程序。这将通过以下方式实现：1)用基因序列数据库编号注释NSRL数据库，并通过iDigBio和VertNet等数据门户提供这些数据；2)在国家科学研究实验室的内部数据库中标注从病毒阳性凭单中获得的样本，并用颜色编码标记这些样本；3)用彩色编码标签识别共生型和全型遗传样本。总体而言，该项目旨在保护标本的完整性和信息内容，同时通过突出与他们生活相关的问题来吸引更广泛的社区。