Alternative Polyadenylation as a Major Regulatory Mechanism of Plant Innate Immunity

替代多腺苷酸化作为植物先天免疫的主要调节机制

• 批准号: 1457329
• 负责人: Thomas Eulgem
• 金额: $ 60.98万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2020-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1457329&HistoricalAwards=false
• 关键词: Alternative; Polyadenylation; Major; Regulatory; Mechanism

Plant disease resistance (R)-genes encode immune receptors that mediate disease resistance upon recognition of pathogenic microorganisms. Their expression has to be coordinated. The investigators previously identified a new process that controls the expression of two R-genes in the model plant Arabidopsis thaliana. The main goals of this project are (1) to examine extent to which this new mechanism is utilized in plants for the regulation of additional R-genes and other defense genes and (2) to gain detailed understanding of this new mechanism. Results from this study are likely to have a broad impact on the fields of plant immunity and gene expression control. This project may also lead to the design of new strategies for enhanced disease protection in crop plants. An important component of the project is major involvement of undergraduate students, mainly from minority groups. The project will be linked to classes on biochemistry and biotechnology taught by the Principal Investigator and the ongoing NSF-REU Plant Cell Biology program within the Center for Plant Cell Biology at UC-Riverside. In addition, the project will provide a strong platform for training of postdoctoral scholars and graduate students and prepare them for careers as senior researchers in academia or industry. The goals of the America COMPETES Act of 2007 that emphasize awareness of the responsible and ethical conduct of research are a top priority of this project.Alternative polyadenylation is emerging as a major mechanism of eukaryotic gene expression control. The investigators previously found the epigenetic mark H3K9me2 to control differential use of polyadenylation signals of the Arabidopsis R-genes RPP4 and RPP7 and to modulate levels of immunity mediated by their encoded immune receptors. This mechanism is controlled by the epigenetic factor EDM2 and several additional newly identified regulators. The investigators further showed this novel mechanism to be responsive to pathogen recognition and to dynamically adjust RPP4 and RPP7 expression. The central hypothesis of this project is that alternative polyadenylation is a major mechanism controlling inducible plant immune responses. The two specific aims that are to be pursued are: 1) Genome wide profiling of alternative polyadenylation events after defense induction; 2) Identify regulatory factors linking alternative polyadenylation to pathogen perception and defense signaling. Alternative polyadenylation has so far not been recognized as a major mechanism controlling plant immunity. This project will provide a genome-wide overview about defense-associated alternative polyadenylation in plants and uncover molecular details of this type of regulatory process.

植物抗病性（R）-基因编码免疫受体，其在识别病原微生物时介导抗病性。它们的表达必须协调一致。研究人员先前确定了一个控制模式植物拟南芥中两个R基因表达的新过程。该项目的主要目标是（1）检查这种新机制在植物中用于调节额外的R基因和其他防御基因的程度，以及（2）详细了解这种新机制。这项研究的结果可能会对植物免疫和基因表达控制领域产生广泛的影响。该项目还可能导致设计新的战略，以加强作物植物的疾病保护。该项目的一个重要组成部分是主要来自少数群体的大学生的主要参与。该项目将与主要研究者教授的生物化学和生物技术课程以及加州大学河滨分校植物细胞生物学中心正在进行的NSF-REU植物细胞生物学课程相联系。此外，该项目将为博士后学者和研究生的培训提供一个强大的平台，并为他们在学术界或工业界担任高级研究人员做好准备。2007年美国竞争法的目标，强调负责任和道德的研究行为的意识是这个项目的重中之重。替代多聚腺苷酸化正在成为真核基因表达控制的主要机制。研究人员先前发现表观遗传标记H3 K9 me 2控制拟南芥R基因RPP 4和RPP 7的多聚腺苷酸化信号的差异使用，并调节由其编码的免疫受体介导的免疫水平。这种机制是由表观遗传因子EDM 2和几个新发现的调节因子控制的。研究人员进一步表明，这种新机制对病原体识别有反应，并动态调节RPP 4和RPP 7的表达。该项目的中心假设是，选择性多聚腺苷酸化是控制诱导型植物免疫反应的主要机制。要追求的两个具体目标是：1）防御诱导后替代多聚腺苷酸化事件的全基因组谱分析; 2）鉴定将替代多聚腺苷酸化与病原体感知和防御信号传导联系起来的调节因子。 选择性多聚腺苷酸化迄今尚未被认为是控制植物免疫的主要机制。该项目将提供一个全基因组的概述防御相关的替代多聚腺苷酸化在植物和揭示这种类型的调控过程的分子细节。