RUI: Collaborative Research: Phylogenomics of Anthozoa (Cnidaria): new approaches to long-standing problems

RUI：合作研究：珊瑚虫（刺胞动物）的系统基因组学：解决长期存在问题的新方法

• 批准号: 1457817
• 负责人: Catherine McFadden
• 金额: $ 49.45万
• 依托单位: Harvey Mudd College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2020-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1457817&HistoricalAwards=false
• 关键词: RUI; Collaborative; Research; Phylogenomics; Anthozoa

Worldwide, humans and countless other species are dependent on coral reefs for shelter, sustenance and livelihoods. Increasing atmospheric carbon dioxide is causing the world's oceans to become warmer and more acidic, a chemical change that may prevent corals from forming calcium carbonate skeletons. The fossil record indicates, however, that some groups of corals have survived similar environmental crises in past geological eras, and that changes in ocean chemistry may result in the evolution of different types of skeletons or of corals that lack skeletons. Understanding these past evolutionary transitions and the environmental conditions under which they occurred may help scientists predict the responses of today's reef-building corals to future climate change. This collaborative project between researchers from Harvey Mudd College, a Principally Undergraduate Institution, and the American Museum of Natural History will investigate the evolution of calcium carbonate skeletons in Anthozoa (corals, sea anemones, and relatives). They will first generate an extensive time-calibrated molecular phylogeny of the group and then use this evolutionary framework to study the evolution of skeletal characters. Students from groups underrepresented in the sciences will participate in this research through the PI's mentoring of undergraduates at the minority-serving New York City College of Technology, and the Scripps College Academy, a program for high school girls in the Los Angeles area. The project will also generate diverse outreach materials for a public display on corals at the American Museum of Natural History. Although previous molecular phylogenetic studies have found strong support for relationships among some orders of Anthozoa, key regions of the tree remain poorly resolved, impeding efforts to understand character evolution within the group. By first sequencing complete genomes from eight distantly related taxa of Anthozoa researchers will then design a set of Ultra-Conserved Elements (UCEs) that can be used throughout Anthozoa. UCE sequences will then be generated for 192 Anthozoa species spanning diversity within the group to generate the first phylogenomic estimate of relationships within the group. The researchers will then use this phylogenetic tree and a diverse set of comparative methods to infer the direction, timing and paleoclimatic correlates of evolutionary transitions in skeletogenesis and other traits within the clade that have allowed anthozoans to engineer the largest biological structures on the planet.

在世界范围内，人类和无数其他物种依赖珊瑚礁提供住所、食物和生计。大气中二氧化碳的增加导致世界海洋变得更加温暖和酸性，这种化学变化可能会阻止珊瑚形成碳酸钙骨架。然而，化石记录表明，在过去的地质时代，一些珊瑚群在类似的环境危机中幸存下来，海洋化学的变化可能导致不同类型的骨骼或缺乏骨骼的珊瑚的进化。了解这些过去的进化转变及其发生的环境条件可能有助于科学家预测今天的造礁珊瑚对未来气候变化的反应。来自Harvey Mudd学院（一所主要的本科院校）和美国自然历史博物馆的研究人员之间的这个合作项目将调查珊瑚虫（珊瑚，海葵和亲戚）中碳酸钙骨骼的演变。 他们将首先生成一个广泛的时间校准的群体分子进化，然后使用这个进化框架来研究骨骼特征的进化。来自科学领域代表性不足的群体的学生将通过PI对少数民族服务的纽约市理工学院和斯克里普斯学院的本科生的指导参与这项研究，斯克里普斯学院是洛杉矶地区的高中女生项目。该项目还将制作各种宣传材料，在美国自然历史博物馆公开展示珊瑚。虽然以前的分子系统发育研究已经发现了珊瑚虫的一些订单之间的关系的强有力的支持，树的关键区域仍然解决不好，阻碍了努力了解组内的字符进化。首先对珊瑚虫门八个远亲分类群的完整基因组进行测序，然后研究人员将设计一组可在整个珊瑚虫门中使用的超保守元件（UCE）。 UCE序列，然后将产生192个珊瑚虫物种跨越组内的多样性，以产生组内的关系的第一个基因组估计。 然后，研究人员将使用这种系统发育树和一套不同的比较方法来推断骨骼发育和分支内其他特征的进化转变的方向，时间和古气候相关性，这些特征使珊瑚虫能够设计地球上最大的生物结构。