权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Elucidating novel mechanisms of plastid gene function and regulation by an in-depth characterization of the Oenothera (evening-primrose) plastome mutant collection

通过月见草（月见草）质体突变体集合的深入表征，阐明质体基因功能和调控的新机制

基本信息

批准号：
218849174
负责人：
Dr. Stephan Greiner
金额：
--
依托单位：
Max-Planck-Institut für molekulare Pflanzenphysiologie (MPI-MP)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/218849174?language=en
关键词：
Elucidating novel mechanisms plastid gene

项目摘要

Reports on mutants of the chloroplast genomes (plastome) coincide with the early rise of genetics. Ever since, they provided a valuable material to study cytoplasmic inheritance. However, due to previous technical limitations, they currently have made only a minor contribution to research in physiology and molecular biology. Here, we present a fully characterized collection of 51 plastome mutants from the genus Oenothera (evening primrose) that provides valuable material for future research on plastid genetics, gene expression and photosynthesis. By characterizing the mutant material of the collection, we could demonstrate the presence of programmed ribosomal frameshifting in the chloroplast genome, a novel mechanism of plastid gene regulation. With the research proposed here, our aim is now to elucidate the functional role of programmed ribosomal frameshifting in chloroplast translation. By investigating the Oenothera plastome mutant collection, transplastomic tobacco lines, as well as suitable wild-type loci we want to clarify what are the genetic consequences of programmed ribosomal frameshifting in the chloroplast and elucidated its role in the regulation of chloroplast translation.

关于叶绿体基因组突变体的报道与遗传学的早期兴起相吻合。从那时起，它们为研究细胞质遗传提供了宝贵的材料。然而，由于以前的技术限制，他们目前只对生理学和分子生物学的研究做出了很小的贡献。在这里，我们提出了一个充分的特点收集51质体突变体属月见草（月见草报春花），为今后的研究提供了宝贵的材料，质体遗传学，基因表达和光合作用。通过表征收集的突变体材料，我们可以证明叶绿体基因组中存在程序性核糖体移码，这是质体基因调控的一种新机制。通过本研究，我们的目标是阐明程序性核糖体移码在叶绿体翻译中的功能作用。通过调查月见草质体突变体收集，transplastomic烟草线，以及合适的野生型位点，我们要澄清什么是程序性核糖体移码在叶绿体中的遗传后果，并阐明其在叶绿体翻译的调节作用。