Cloning and functional characterization of the vec1 resistance gene

vec1抗性基因的克隆和功能表征

• 批准号: 219001547
• 负责人: Dr. Elke Diederichsen
• 金额: --
• 依托单位: Abteilung Molekulare Phytopathologie und Mykotoxinforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/219001547?language=en
• 关键词: Cloning; functional; characterization; vec1; resistance

The first funding period resulted in the identification of several major QTL controlling multiple disease parameters such as resistance to systemic spread or to stunting caused by V. longisporum in Arabidopsis and Brassica. The focus of the new application is the molecular identification of the responsible gene(s) in Arabidopsis. A combination of different approaches will be pursued to achieve the cloning of a gene which is controlling host resistance against systemic spread of the fungus. The basis of these approaches will be the continuation of fine-mapping in a population of NILs which differ only in a small segment on chromosome 2 which carries at least one gene controlling spreading resistance (vec1) and also the erecta locus. The latter has been shown to be responsible for stunting resistance. Fine-mapping and expression studies in tailor-made NILs (infected vs. non-infected) will allow identifying a small number of candidate genes. To identify vec1 these will be cloned and analysed for their effect on spreading resistance after complementation in the susceptible ecotype Ler. The effect of vec1 on defence signalling will be studied in expression studies and grafting experiments. Histological defence reactions will be studied microscopically. Homologous genes in Brassica will be searched and analysed for their correlation to resistance in existing mapping populations of Brassica crops, which will allow the application of our results in oilseed rape breeding.

第一个资助期鉴定了几个控制多个疾病参数的主要QTL，例如对拟南芥和芸苔属中长孢弧菌引起的系统传播或发育迟缓的抗性。新应用的重点是拟南芥中负责基因的分子鉴定。将寻求不同方法的组合，以实现控制宿主对真菌系统性传播的抗性的基因的克隆。这些方法的基础将是继续在近等基因系群体中进行精细定位，这些近等基因系仅在2号染色体上的一小段上不同，该小段携带至少一个控制扩展抗性的基因（vec1）以及直立基因座。后者已被证明是负责发育迟缓的阻力。在定制的近等基因组（感染与未感染）中进行精细定位和表达研究将允许鉴定少量候选基因。为了鉴定vec1，将克隆这些基因并分析它们在易感生态型Ler中互补后对扩展抗性的影响。将在表达研究和移植实验中研究vec1对防御信号传导的影响。将在显微镜下研究组织学防御反应。将在芸苔属作物的现有作图群体中搜索和分析芸苔属中的同源基因与抗性的相关性，这将允许我们的结果在油菜育种中的应用。