DISSERTATION RESEARCH: Consequences of sympatry and allopatry for variation in reproductive genes of Drosophila pseudoobscura

论文研究：同源性和异源性对果蝇生殖基因变异的影响

• 批准号: 1500911
• 负责人: Leonie Moyle
• 金额: $ 2.01万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1500911&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Consequences; sympatry; allopatry

Many factors influence mate choice in both plants and animals, including choosing a mate that will provide the best quality offspring while avoiding individuals of different species. The integration of these factors into a single choice implies that traits involved in mate selection within species could be the same traits that cause species to diverge from one another. Recently, the investigators used genetically modified Drosophila melanogaster to show that the same genes that control within species mate choice are also involved in species discrimination. This project is testing this association in a natural system, to understand how different populations accumulate changes in genes that control mate choice and species discrimination. These data will provide insight into the molecular mechanisms underlying natural variation in mate choice and the formation of reproductively isolated populations, which are important factors in understanding how new species arise over evolutionary time. The proposed research will examine DNA sequence and gene expression variation in reproductive genes in natural populations of the fruit fly Drosophila pseudoobscura, using high depth nucleotide sequencing. The experiment contrasts genetic changes that have occurred in populations that co-occur with closely related species, and thus must discriminate against them during mate choice, with changes in populations that are not exposed to other species. Analyses will assess genetic variation across all reproductive genes, as well as specifically in genes known to affect gamete competition. These variants will be associated with phenotypic data on mate choice within and between species, to differentiate genes that are important to mate choice, to species discrimination, and to both reproductive behaviors.

许多因素影响植物和动物的配偶选择，包括选择一个配偶，这将提供最好的质量后代，同时避免不同物种的个体。将这些因素整合到一个单一的选择中意味着，物种内涉及配偶选择的特征可能与导致物种彼此分化的特征相同。最近，研究人员使用转基因果蝇来表明，控制物种内配偶选择的相同基因也参与了物种歧视。该项目正在自然系统中测试这种关联，以了解不同种群如何积累控制配偶选择和物种歧视的基因变化。这些数据将提供深入了解配偶选择和生殖隔离种群形成的自然变异的分子机制，这是理解新物种如何在进化过程中出现的重要因素。拟议的研究将使用高深度核苷酸测序来检查果蝇（Drosophila pseudobscura）自然种群生殖基因的DNA序列和基因表达变异。该实验对比了与密切相关的物种共同出现的种群中发生的遗传变化，因此必须在配偶选择过程中对它们进行歧视，并与未暴露于其他物种的种群中的变化进行比较。分析将评估所有生殖基因的遗传变异，特别是已知影响配子竞争的基因。这些变异将与物种内和物种间的择偶表型数据相关联，以区分对择偶、物种歧视和生殖行为都很重要的基因。