DISSERTATION RESEARCH: Small RNA regulation and the evolution of extreme anoxia tolerance

论文研究：小RNA调控和极端缺氧耐受性的进化

• 批准号: 1501414
• 负责人: Jason Podrabsky
• 金额: $ 1.93万
• 依托单位: Portland State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-15 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1501414&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Small; RNA; regulation

This project will trace the evolution of low-oxygen tolerance in vertebrates. The study may reveal novel cellular mechanisms underpinning anoxia tolerance, which may transform our prevention and treatment of heart attack and stroke. Humans, and most other vertebrates, can survive for only a few minutes without adequate blood flow to the heart and brain. Loss of blood flow blocks the supply of oxygen, and quickly leads to tissue anoxia, or lack of oxygen. Yet, there are a handful of vertebrates that can survive for months in the complete absence of oxygen. This project examines and compares the expression of genes involved in anoxia tolerance of species from each major vertebrate group. The project will also promote international scientific collaboration and provide training of a female Ph.D. candidate and undergraduate student. Additionally, research findings will be presented to a Science Research Methods class at St. Mary?s Academy, a local all-girls high school.To study small RNA expression and the evolution of anoxia tolerance, small RNA expression will be examined in the epaulette shark, crucian carp, annual killifish embryos, leopard frog, and freshwater turtles. These species represent the most anoxia-tolerant cartilaginous fish, bony fishes, amphibians and reptiles, respectively. This research tests the hypothesis that common small RNAs support the anoxia tolerance of these few most tolerant vertebrates, indicating a convergent evolution of the life history trait. Known hypoxia-responsive and novel anoxia-responsive small RNAs were recently identified in the anoxia tolerant annual killifish Austrofundulus limnaeus. This project will test the role of small RNAs in the evolution and maintenance of anoxia tolerance by comparing them among anoxia-tolerant vertebrate species. Each species will be exposed to anoxia and recovery at its ecologically relevant temperature and sampled at 3 time points: A.) prior to anoxia; B.) during anoxia; C.) during aerobic recovery following anoxia. The specific exposure intervals are set based on the maximal anoxia tolerance of each species. Total RNA will be extracted from brain tissue, since the brain is the most anoxia-sensitive organ, and prepared for small RNA sequencing, and expression patterns will be compared.

该项目将追踪脊椎动物低氧耐受性的演变。这项研究可能揭示支持缺氧耐受的新细胞机制，这可能会改变我们对心脏病发作和中风的预防和治疗。人类和大多数其他脊椎动物在没有足够血液流向心脏和大脑的情况下只能存活几分钟。血液流动的损失阻碍了氧气的供应，并迅速导致组织缺氧或缺氧。然而，有少数脊椎动物可以在完全没有氧气的情况下存活数月。本计画检视并比较各主要脊椎动物群中与耐缺氧相关基因的表现。该项目还将促进国际科学合作，并培训一名女博士。候选人和本科生。此外，研究结果将提交给科学研究方法类在圣玛丽？为了研究小RNA的表达和缺氧耐受性的进化，将在肩章鲨、鲫鱼、一年生鳉鱼胚胎、豹蛙和淡水龟中检测小RNA的表达。这些物种分别代表最耐缺氧的软骨鱼、硬骨鱼、两栖动物和爬行动物。这项研究验证了一个假设，即常见的小RNA支持这些最耐受的脊椎动物的缺氧耐受性，表明生活史特征的趋同进化。已知的缺氧反应和新的缺氧反应的小RNA最近被确定在耐缺氧的一年一度的鳉鱼Austrofundulus limnaeus。该项目将通过比较耐缺氧脊椎动物物种中的小RNA来测试它们在缺氧耐受性的进化和维持中的作用。每个种属将暴露于缺氧环境，并在其生态相关温度下恢复，并在3个时间点取样：A.）缺氧前; B.）缺氧时; C.）在缺氧后的有氧恢复期间。具体的暴露间隔是根据每个物种的最大耐缺氧能力设定的。由于大脑是对缺氧最敏感的器官，因此将从脑组织中提取总RNA，并制备用于小RNA测序，并将比较表达模式。