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Volatile organic compounds (VOC) of pathogen and host - potential biomarkers of bacterial infections

病原体和宿主的挥发性有机化合物 (VOC) - 细菌感染的潜在生物标志物

基本信息

批准号：
219411486
负责人：
Professorin Dr. Petra Reinhold, Ph.D.
金额：
--
依托单位：
Friedrich-Loeffler-Institut - Bundesforschungsinstitut für Tiergesundheit (FLI)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2017-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/219411486?language=en
关键词：
Volatile organic compounds VOC pathogen

项目摘要

Within this project that started in May 2012 volatile organic compounds (VOCs) were identified as biomarkers of bacterial infection. These markers enabled differentiation of genetically different strains of Mycobacterium avium subsp. paratuberculosis (MAP) and characterization of different growth rates or the density of bacterial growth, respectively. In addition, these markers could be used to recognize infection and host responses in living animals. The future experiments will again focus on MAP, and the main goal will be to evaluate suitable VOC marker sets in livestock. For that purpose, animals (goats and cattle) with naturally acquired MAP infections will be studied. Determination of VOC profiles from breath and feces of these animals shall prove whether results from the experimental infection model (MAP infected goats) can be transferred to other species (esp. cattle) and to MAP-infections present in the field. In detail, the following issues will be addressed.- In goat flocks and dairy herds with naturally occurring MAP-infections, VOC profiles will be determined from breath and feces of individual animals. VOC results will be compared with results from bacterial cultures grown from feces of these animals. In this way, co-infections will be excluded or taken into account (if present).- As a complementary approach, native tissues and feces obtained from those goats and cows (either MAP-infected or non-infected) will be analyzed in order to assess matrix effects onto VOC profiles in biological samples.- Continuous sampling by means of real time mass spectrometry in the flocks or herds, respectively, will provide substantial information about robustness of the VOC markers and their circadian and/or day-to-day variability under field conditions. - In addition, VOC analysis of ambient air in animal housings will be performed in order to evaluate the option of using volatile signatures present in stable air as a simplified diagnostic approach for MAP detection on the herd level. In a perspective view, this project will generate sound scientific evidence as a basis for the development of simple and fast systems for non-invasive detection of MAP infections in livestock as well as for the in vitro detection of MAP growth in samples obtained from the field.

在2012年5月开始的这个项目中，挥发性有机化合物（VOC）被确定为细菌感染的生物标志物。这些标志物能够区分鸟分枝杆菌亚种的遗传上不同的菌株。副结核杆菌（MAP）和表征不同的生长速率或细菌生长的密度。此外，这些标记物可用于识别活体动物中的感染和宿主反应。未来的实验将再次集中在MAP上，主要目标将是评估牲畜中合适的VOC标记集。为此，将研究自然获得MAP感染的动物（山羊和牛）。确定这些动物呼吸和粪便中的VOC分布应证明实验感染模型（MAP感染的山羊）的结果是否可以转移到其他物种（特别是牛）和现场存在的MAP感染。具体而言，将讨论以下问题。在自然发生MAP感染的山羊群和奶牛群中，将根据个体动物的呼吸和粪便确定VOC特征。将VOC结果与从这些动物粪便中生长的细菌培养物的结果进行比较。通过这种方式，将排除或考虑合并感染（如果存在）。作为补充方法，将分析从这些山羊和奶牛（MAP感染或未感染）获得的天然组织和粪便，以评估基质对生物样品中VOC特征的影响。通过分别在羊群或牛群中进行真实的时间质谱法连续采样，将提供关于VOC标记物的稳健性及其在田间条件下的昼夜节律和/或日间变化的大量信息。- 此外，还将对畜舍中的环境空气进行VOC分析，以评价使用稳定空气中存在的挥发性特征作为牛群水平MAP检测的简化诊断方法的选项。从长远来看，该项目将产生合理的科学证据，作为开发简单快速的系统的基础，用于非侵入性检测牲畜中的MAP感染，以及从现场获得的样品中的MAP生长的体外检测。