Collaborative Research: Is nitrogen fixation widespread in the Chukchi and Beaufort Seas?

合作研究：固氮作用在楚科奇海和波弗特海广泛存在吗？

• 批准号: 1503614
• 负责人: Jonathan Zehr
• 金额: $ 24.15万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-12-01 至 2020-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1503614&HistoricalAwards=false
• 关键词: Collaborative; Research; nitrogen; fixation; widespread

The extent of summer Arctic sea ice loss is increasing and now occurs earlier in the year. As a result, it is predicted that the rate of growth of phytoplankton, the base of the marine food web that sustains subsistence marine harvests by native populations, will increase within the Arctic seas. The limited amount of available nitrogen, a required nutrient for phytoplankton, eventually will restrict the level of growth. Nitrogen gas dissolved in the ocean can be converted to a form readily utilizable by phytoplankton, but this has been considered primarily a warm-water process. The principal investigators of this project recently have observed this process in the Arctic Ocean, but there is so little data that its extent remains highly speculative. If it is widespread, it will change the way we think about future scenarios for the changing Arctic marine ecosystem, subsistence fishing, and, potentially, commercial fishing in the Arctic. The project will also contribute to workforce development. The principal investigator is an early-career, female scientist. She will use the project as a mechanism to entrain an undergraduate student into research. She will also use the project to sustain an existing educational collaboration between the Virginia Institute of Marine Sciences and Hampton University, a historically black university.It is hypothesized that microorganisms capable of fixing N2 (diazotrophs) are present in the Chukchi and Beaufort Seas, that they produce measurable rates of N2 fixation in near shore and offshore Arctic marine waters and that diazotroph community composition will differ between coastal sites, which are influenced by terrestrial inputs, versus open water sites. The Chukchi and Beaufort Seas will be sampled during a cruise in late summer 2016, when hydrographic and nutrient conditions are likely to favor diazotrophic populations. The impact that N2 fixation will have on Arctic ecosystems is dependent on its rate, spatial extent and the conditions that favor it. As a consequence, on each cruise the PIs propose to determine diazotroph community composition, examine their distributions based on the presence of the nitrogenase gene (nifH), measure rates of primary productivity and uptake of inorganic and organic N and C substrates using 15N and 13C tracer techniques, and to compare these to hydrographic, nutrient, and overall microbial community composition profiles made along cruise transects. The proposed work will determine the extent of active N2 fixation within the region in the context of other key biogeochemical and microbial community parameters.

夏季北极海冰损失的程度正在增加，现在出现在一年中的早些时候。 因此，据预测，浮游植物的增长率将在北极海域内增加，而浮游植物是维持当地人口海洋捕捞生计的海洋食物网基础。 浮游植物所需的营养素--可利用氮的数量有限，最终将限制其生长水平。 溶解在海洋中的氮气可以转化为浮游植物容易利用的形式，但这主要被认为是一个温水过程。 该项目的主要研究人员最近在北冰洋观察到了这一过程，但数据非常少，其程度仍然高度推测。 如果它广泛传播，它将改变我们对北极海洋生态系统变化、自给性渔业以及北极潜在商业渔业未来情景的思考方式。 该项目还将促进劳动力发展。 首席研究员是一位职业生涯早期的女性科学家。 她将利用这个项目作为一种机制，引导一名本科生进行研究。 她还将利用这个项目来维持弗吉尼亚海洋科学研究所和汉普顿大学之间现有的教育合作，汉普顿大学是一所历史悠久的黑人大学。（固氮生物）存在于楚科奇海和博福特海，它们在近海岸和北极近海沃茨中产生可测量固氮率，固氮生物群落组成将不同受陆地输入影响的沿海地点与开放水域地点之间的差异。楚科奇海和博福特海将在2016年夏末的一次巡航中取样，届时水文和营养条件可能有利于重氮营养种群。固氮对北极生态系统的影响取决于固氮的速率、空间范围和有利条件。因此，在每次巡航中，PI建议确定固氮生物群落组成，根据固氮酶基因（nifH）的存在检查其分布，利用15 N和13 C示踪技术测量初级生产力和无机和有机N和C基质的吸收率，并将其与水文，营养，以及沿沿着巡航样带的总体微生物群落组成概况。拟议的工作将在其他关键的生物地球化学和微生物群落参数的背景下，确定该区域内活跃的N2固定程度。