Photochemical Strategies with Supramolecular Assistance to Monitor Cellular Dynamics in Living Organism

超分子辅助光化学策略监测活体细胞动力学

• 批准号: 1505885
• 负责人: Francisco Raymo
• 金额: $ 45万
• 依托单位: University of Miami
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-01-15 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1505885&HistoricalAwards=false
• 关键词: Photochemical; Strategies; Supramolecular; Assistance; Monitor

This project aims at the design and preparation of molecules that can be transported to target locations in organisms and emit light only after stimulation with an activating optical beam. Subsequent detection of the emitted light offers the opportunity to locate the position of the activated molecules in a sample of interest and follow their movements in real time. These light emitters can ultimately become analytical tools for biological studies, such as the tracking of moving cells during the growth of the organism, and contribute valuable insights on organismal development. These fundamental studies in chemistry may have implications in biology and medicine. Additionally, the activities teach the participating undergraduate and graduate students how to prepare molecules from commercial reagents, characterize their structures and investigate their interactions with light. Furthermore, they contribute to strengthening an existing educational collaboration between the laboratory of the principal investigator at the University of Miami and a faculty member of Miami-Dade College. This collaboration exposes high-school and undergraduate students to research. Considering that both institutions have minority enrollments in excess of 50%, these training opportunities may have a significant educational impact on members of underrepresented groups.The goal of this research is the development of biocompatible probes with photoactivatable fluorescence in the deep-red and near-infrared regions of the electromagnetic spectrum. Specifically, this project involves the synthesis of molecules incorporating a fluorescent borondipyrromethene chromophore and a photoswitchable oxazine auxochrome, together with their photochemical/photophysical characterization and incorporation within polymer nanoparticles. Upon illumination at an appropriate activation wavelength, the photoinduced and irreversible opening of the oxazine heterocycle of these compounds is expected to extend the electronic conjugation of the adjacent borondipyrromethene chromophore. This structural transformation is designed to bathochromically shift the main absorption band of the latter component and allow its selective excitation at a suitable wavelength with concomitant fluorescence. On the basis of these operating principles, the translocation of the photochemical and emissive product, across a given sample of interest, can then be probed in real time with the sequential acquisition of fluorescence images. These light emitters can ultimately become analytical tools for biological studies, such as the tracking of moving cells during the growth of the organism, and contribute valuable insights on organismal development.

该项目的目的是设计和制备分子，这些分子可以被运送到生物体中的目标位置，并且只有在用激活光束刺激后才能发光。 随后对发射光的检测提供了定位感兴趣的样品中的激活分子的位置并真实的跟踪它们的运动的机会。 这些光发射器最终可以成为生物学研究的分析工具，例如在生物体生长期间跟踪移动细胞，并为生物体发育提供有价值的见解。 这些化学基础研究可能对生物学和医学有影响。 此外，这些活动教参与的本科生和研究生如何从商业试剂中制备分子，表征其结构并研究其与光的相互作用。 此外，它们有助于加强迈阿密大学首席研究员实验室与迈阿密-戴德学院一名教员之间现有的教育合作。 这种合作使高中和本科生接触到研究。 考虑到这两个机构的少数民族入学率超过50%，这些培训机会可能会对代表性不足的群体成员产生重大的教育影响。本研究的目标是开发在电磁波谱的深红色和近红外区域具有光激活荧光的生物相容性探针。 具体而言，该项目涉及的分子的合成，将荧光borondipyrromethene发色团和photoswitchable恶嗪auxochrome，连同它们的光化学/光物理表征和聚合物纳米颗粒内的掺入。 在适当的活化波长下照射后，这些化合物的恶嗪杂环的光诱导的和不可逆的打开预期将延长相邻硼二吡咯亚甲基发色团的电子共轭。 这种结构转换的目的是向红移后一种成分的主要吸收带，并允许其选择性激发在一个合适的波长伴随荧光。 在这些操作原理的基础上，光化学和发射产物在给定的感兴趣的样品上的移位然后可以通过荧光图像的顺序采集来真实的实时探测。 这些光发射器最终可以成为生物学研究的分析工具，例如在生物体生长期间跟踪移动细胞，并为生物体发育提供有价值的见解。