UNS:Novel biomaterials with light-controlled CO release for modulation of endothelial cells

1510003 Bashur，Christopher A.该奖项的长期目标是对新的组织工程支架进行基础研究，以提高外周动脉疾病患者的移植物活力。超过30%的患者没有可用于移植手术的可行静脉。在这个奖项中，一氧化碳（CO）从纤维支架的受控递送代表了治疗外周动脉疾病的独特组织工程方法。该奖项支持生成血管移植物的初始步骤和基础科学工作，以更好地了解细胞对支架的反应。此外，这项研究将与本科生和研究生的培训相结合，包括来自代表性不足群体的学生。 所提出的研究的具体目标是确定促进细胞培养中内皮细胞增殖的组织工程支架组合物。所提出的支架的一个变革性特征是掺入独特的光活化CO释放分子（photoCORM）。这种策略提供了一种纤维支架，具有控制天然存在的生物信号分子如一氧化碳向周围细胞的递送的能力。具体任务包括：（a）确定支持该photoCORM技术的电纺支架组合物，（B）研究局部CO剂量大小和时间如何影响内皮细胞功能，和（c）设计和合成用于更有效的CO递送的新型photoCORM。内皮细胞将单独培养和共培养，共培养更能代表体内环境。这项基础研究的应用包括外周动脉疾病的治疗。CBET生物技术和生物化学工程项目的这项奖励由材料研究部生物材料项目共同资助。

1510003 Bashur, Christopher A.The long-term objective of this award is to conduct fundamental research into new tissue engineered scaffolds that that could improve graft viability in patients with peripheral artery disease. More than 30% of these patients do not have viable veins that can be used for graft surgeries. The controlled delivery of carbonmonoxide (CO) from a fibrous scaffold in this award represents a unique tissue engineering approach for treating peripheral artery disease. This award supports both the initial steps in generating the vascular graft and the basic science work to better understand the cells response to the scaffold. In addition, this research will be integrated with training of undergraduate and graduate students, including those from underrepresented groups. The specific objective of the proposed research is to determine tissue engineered scaffold compositions that promote endothelial cell proliferation in cell culture. One transformative feature of the proposed scaffold is the incorporation of unique photoactivated CO releasing molecules (photoCORM). This strategy provides a fibrous scaffold with the ability to control delivery of a naturally occurring biological signaling molecule such as carbon monoxide to the surrounding cells. The specific tasks include: (a) determining electrospun scaffold compositions that support this photoCORM technology, (b) investigating how localized CO dose magnitude and timing impacts endothelial cell function, and (c) designing and synthesizing novel photoCORMs for more effective CO delivery. The endothelial cells will be cultured both by themselves and in co-culture, which is more representative of the environment in the body. The applications of this fundamental research include treatment for peripheral artery disease.This award by the Biotechnology and Biochemical Engineering Program of CBET is co-funded by the Biomaterials Program of the Division of Materials Research.