UNS: A Versatile Optofluidic Platform for Multiplexed Detection of Cardiac Biomarkers in Blood

UNS：用于血液中心脏生物标志物多重检测的多功能光流控平台

• 批准号: 1511194
• 负责人: Jianjun Wei
• 金额: $ 30.6万
• 依托单位: University of North Carolina Greensboro
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-15 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1511194&HistoricalAwards=false
• 关键词: UNS; Versatile; Optofluidic; Platform; Multiplexed

PI: Wei, Jianjun Proposal: 1511194The goal is to develop a novel platform that incorporates an optical transmission sensing scheme with an automated size-dependent sample delivery system in a single nanoscale unit. The results will lead to a chip-based, point-of-care (POC) technology for early diagnosis of cardiovascular disease (CVD).This project will investigate a plasmonic nano-optofluidic (PNOF) platform to demonstrate its utility and versatility of in-situ cell separation and delivery of small molecules to the sensing area, and discriminative, sensitive quantitation of protein biomarkers in whole blood. Unique metal film structures will be used as plasmonic waveguides to tune the spectrum that enables ultrasensitive and high signal-to-noise (S/N) ratio detection. The array will be utilized to integrate with a microfluidic network to realize in-situ separation of cells and size-exclusive delivery biomarkers. The researchers will then adopt the immunoassay format and demonstrate delivery and discrimination of selected biomarkers in human blood.

Pi：魏，建军建议：1511194目标是开发一种新型平台，该平台将光传输传感方案与单个纳米单位中的自动大小相关样品输送系统相结合。这一结果将导致基于芯片的护理点(POC)技术用于心血管疾病(CVD)的早期诊断。本项目将研究等离子体纳米光流控(PNOF)平台，以展示其在原位细胞分离和将小分子输送到传感区域以及全血中蛋白质生物标志物的区分和灵敏定量方面的实用性和多功能性。独特的金属薄膜结构将被用作等离子体波导管，以调谐频谱，从而实现超灵敏和高信噪比(S/N)检测。该阵列将被用来与微流控网络集成，实现细胞的原位分离和大小专一的传递生物标志物。然后，研究人员将采用免疫分析形式，并展示人类血液中选定生物标记物的传递和识别。

项目成果

Plasmon–Exciton Coupling in Photosystem I Based Biohybrid Photoelectrochemical Cells

基于光系统 I 的生物混合光电化学电池中的等离子体激子耦合

• DOI: 10.1021/acsabm.8b00249
• 发表时间: 2018
• 期刊: ACS Applied Bio Materials
• 影响因子: 4.7
• 作者: Zeng, Zheng;Mabe, Taylor;Zhang, Wendi;Bagra, Bhawna;Ji, Zuowei;Yin, Ziyu;Allado, Kokougan;Wei, Jianjun
• 通讯作者: Wei, Jianjun

A plasmonic nanoledge array sensor for detection of anti-insulin antibodies of type 1 diabetes biomarker

• DOI: 10.1088/1361-6528/ab8c05
• 发表时间: 2020-08-07
• 期刊: NANOTECHNOLOGY
• 影响因子: 3.5
• 作者: Bagra, Bhawna;Mabe, Taylor;Wei, Jianjun
• 通讯作者: Wei, Jianjun

Plasmon-Enhanced Fluorescence of Carbon Nanodots in Gold Nanoslit Cavities

• DOI: 10.1021/acs.langmuir.9b00448
• 发表时间: 2019-07-09
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Bagra, Bhawna;Zhang, Wendi;Wei, Jianjun
• 通讯作者: Wei, Jianjun

Surface Plasmon Resonance of A Bimetallic Nanostructured Film for Enhanced Optical Sensitivity

• DOI: 10.1002/slct.201800576
• 发表时间: 2018-03-22
• 期刊: CHEMISTRYSELECT
• 影响因子: 2.1
• 作者: Mabe, Taylor;Zeng, Zheng;Wei, Jianjun
• 通讯作者: Wei, Jianjun

Magnetoreception of Photoactivated Cryptochrome 1 in Electrochemistry and Electron Transfer

• DOI: 10.1021/acsomega.8b00645
• 发表时间: 2018-05
• 期刊: ACS Omega
• 影响因子: 4.1
• 作者: Z. Zeng;Jianjun Wei;Yiyang Liu;Wendi Zhang;T. Mabe