UNS: A Versatile Optofluidic Platform for Multiplexed Detection of Cardiac Biomarkers in Blood

主要研究者：魏建军提案：1511194我们的目标是开发一种新型平台，该平台在单个纳米级单元中结合了光学传输传感方案和自动化尺寸相关样品输送系统。该研究结果将导致基于芯片的床旁（POC）技术用于心血管疾病（CVD）的早期诊断。该项目将研究等离子体纳米光流体（PNOF）平台，以证明其原位细胞分离和将小分子递送到传感区域的实用性和多功能性，以及全血中蛋白质生物标志物的区分性，灵敏定量。独特的金属薄膜结构将被用作等离子体波导，以调整光谱，从而实现超灵敏和高信噪比（S/N）检测。该阵列将用于与微流体网络集成，以实现细胞和尺寸排他递送生物标志物的原位分离。然后，研究人员将采用免疫测定的形式，并展示人类血液中选定的生物标志物的传递和区分。

PI: Wei, Jianjun Proposal: 1511194The goal is to develop a novel platform that incorporates an optical transmission sensing scheme with an automated size-dependent sample delivery system in a single nanoscale unit. The results will lead to a chip-based, point-of-care (POC) technology for early diagnosis of cardiovascular disease (CVD).This project will investigate a plasmonic nano-optofluidic (PNOF) platform to demonstrate its utility and versatility of in-situ cell separation and delivery of small molecules to the sensing area, and discriminative, sensitive quantitation of protein biomarkers in whole blood. Unique metal film structures will be used as plasmonic waveguides to tune the spectrum that enables ultrasensitive and high signal-to-noise (S/N) ratio detection. The array will be utilized to integrate with a microfluidic network to realize in-situ separation of cells and size-exclusive delivery biomarkers. The researchers will then adopt the immunoassay format and demonstrate delivery and discrimination of selected biomarkers in human blood.