Metal/metal and metal/nitroxyl-labeled compounds for the development of EPR-based distance measurement techniques

用于开发基于 EPR 的距离测量技术的金属/金属和金属/硝酰基标记化合物

• 批准号: 221163074
• 负责人: Professorin Dr. Adelheid Godt
• 金额: --
• 依托单位: Arbeitsgruppe Organic and Macromolecular Chemistry
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/221163074?language=en
• 关键词: Metal; metal; nitroxyl; labeled; compounds

Electron paramagnetic resonance (EPR) techniques have become very useful tools to elucidate the structure of (bio)macromolecules and their assemblies in a disordered, native state. An in-cell application is on the horizon. For this purpose the molecules of interest are site-selectively spin labeled. For a long time this was done with nitroxyl radicals. Only recently Gd(III) came into focus as a spin label. One major objective of this project is to identify Gd(III) complexes having the spectroscopical properties required for a specific EPR technique (pulse and cw EPR) and developing them into ready-made spin labels for site-directed spin labeling of proteins. To sound the potential of Gd-based spin labels as well as those of the newly developing Gd-based EPR techniques compounds of the type Gd-spacer-Gd and Gd-spacer-nitroxyl will be prepared. The spacer keeps the two spin labels at a well-defined, tunable distance. The compounds will be water soluble to allow EPR experiments under conditions identical to those when biomolecules are studied. Model compounds with Cu(II) and Mn(II) as spin labels are included as synthetic targets in our project. This enables a broader fundamental exploration of EPR spectroscopical techniques that are applied to paramagnetic metal ions interacting with a second paramagnetic metal ion of the same or different kind or with a nitroxyl radical, a situation met with spin-labeled metallo-proteins.

电子顺磁共振（EPR）技术已经成为非常有用的工具来阐明（生物）大分子的结构及其组装在无序的，天然的状态。一个单元内应用程序即将出现。为此目的，感兴趣的分子被选择性自旋标记。很长一段时间，这都是用硝基自由基来做的。直到最近，Gd（III）才作为一个自旋标签成为人们关注的焦点。该项目的一个主要目标是鉴定具有特定EPR技术（脉冲和连续波EPR）所需的光谱特性的Gd（III）配合物，并将其开发成用于蛋白质定向自旋标记的现成自旋标签。为了探索gd基自旋标记的潜力，以及近年来发展起来的gd基EPR技术，本文将制备gd -间隔剂- gd型和gd -间隔剂-硝基型化合物。间隔器将两个自旋标签保持在一个明确的、可调的距离上。这些化合物将是水溶性的，以便在与生物分子研究相同的条件下进行EPR实验。以Cu（II）和Mn（II）为自旋标签的模型化合物作为我们项目的合成靶点。这使得EPR光谱技术应用于顺磁性金属离子与第二种相同或不同种类的顺磁性金属离子或与硝基自由基相互作用的更广泛的基础探索，这种情况与自旋标记的金属蛋白有关。