Application of Amplitude/Phase Modulated Pulses for Dipolar EPR Spectroscopy

在这个项目中，我们想要开发基于幅度/相位调制的宽带脉冲的新型偶极光谱脉冲序列。这包括一个新的CPMG(Carr-Purcell/Meiom-Gill)脉冲电子双共振(PELDOR)序列，它具有延长的观察时间窗口，一个宽带2D-SIFTER(用于偶极耦合重聚焦的单频技术)脉冲序列，用于带有偶极时间迹中编码的取向信息的刚性自旋标记，以及DQC(双量子相干)实验，该实验可能扩展到多量子过滤器实验，这将是确定特定条件下膜蛋白质复合体低聚态变化的有价值的工具。此外，我们还想探索在非常高的磁场(9.2T)下产生这样的脉冲序列的可能性。

In this project we want to develop new pulse sequences for dipolar spectroscopy based on amplitude/phase modulated broadband pulses. This includes a new CPMG (Carr-Purcell / Meiboom-Gill) PELDOR (Pulsed Electron Electron Double Resonance) sequence, which exhibits a prolonged observation time window, a broadband 2D-SIFTER (Single Frequency Technique for Refocusing of Dipolar Coupling) pulse sequence for rigid spin labels with orientation information encoded in the dipolar time traces and a DQC (Double Quantum Coherence) experiment, which might be extended to multiple-quantum filter experiments, which would be valuable tools for determining the change of oligomeric states of membrane protein complexes under specific conditions. Additionally we want to explore the possibility of such pulse sequences at very high magnetic fields (9.2 T).