Application of Amplitude/Phase Modulated Pulses for Dipolar EPR Spectroscopy

调幅/调相脉冲在偶极 EPR 光谱中的应用

• 批准号: 221296566
• 负责人: Professor Dr. Thomas F. Prisner
• 金额: --
• 依托单位: Institut für Physikalische und Theoretische Chemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/221296566?language=en
• 关键词: Application; Amplitude; Phase; Modulated; Pulses

In this project we want to develop new pulse sequences for dipolar spectroscopy based on amplitude/phase modulated broadband pulses. This includes a new CPMG (Carr-Purcell / Meiboom-Gill) PELDOR (Pulsed Electron Electron Double Resonance) sequence, which exhibits a prolonged observation time window, a broadband 2D-SIFTER (Single Frequency Technique for Refocusing of Dipolar Coupling) pulse sequence for rigid spin labels with orientation information encoded in the dipolar time traces and a DQC (Double Quantum Coherence) experiment, which might be extended to multiple-quantum filter experiments, which would be valuable tools for determining the change of oligomeric states of membrane protein complexes under specific conditions. Additionally we want to explore the possibility of such pulse sequences at very high magnetic fields (9.2 T).

在这个项目中，我们希望开发新的脉冲序列的偶极光谱的基础上幅度/相位调制的宽带脉冲。这包括一个新的CPMG（Carr-Purcell / Meiboom-Gill）PELDOR（脉冲电子电子双共振）序列，它表现出延长的观察时间窗口，宽带2D-SIFTER（用于偶极耦合的重聚焦的单频技术）用于刚性自旋标记的脉冲序列，其具有在偶极时间迹线中编码的取向信息和DQC（双量子相干）实验，该实验可扩展为多量子滤波实验，这将是确定特定条件下膜蛋白复合物寡聚态变化的有价值的工具。此外，我们想探索这种脉冲序列在非常高的磁场（9.2 T）的可能性。