Identification of novel regulators of stem cell self-renewal in the larval Drosophila CNS

果蝇幼虫神经母细胞（NB）已被广泛用作研究控制细胞分裂和细胞命运决定机制的模型系统。神经节母细胞（神经节母细胞，GMC）是神经节母细胞（神经节母细胞）。一系列研究表明，细胞命运决定因子Prospero、Brat和Numb在细胞向GMC的非对称分裂过程中分离，并改变了细胞分化的转录特征。与此相反，我们对NBs中如何维持自我更新或干细胞身份知之甚少。为了揭示与NBs自我更新有关的新因素，我们通过下一代mRNA测序对facs分类的NBs进行了转录组分析。我们将这些数据与facs分类神经元的mRNA测序数据进行比较，以丰富NBs中特异性表达的基因。本应用项目详细分析来自转录组数据的不同基因，研究它们在NB自我更新和NB谱系身份确定中的功能，以表征幼虫中枢神经系统中单个NB谱系。

Drosophila larval neuroblast (NB) have intensively been used as a model system to study mechanisms that control cell division and cell fate decisions. The NBs divide asymmetrically to self-renew and to generate one differentiating daughter cell, the ganglion mother cell (GMC). A series of studies showed, that cell fate determinants Prospero, Brat and Numb segregate during asymmetric cell division to the GMC and change the transcriptional profile towards differentiation. In contrast to this not much is known on how self-renewal or stem cell identity is maintained in the NBs. To unravel new factors involved in NBs self-renewal we have undertaken a transcriptome analysis of FACS-sorted NBs via next generation mRNA sequencing. We compared these data to mRNA sequencing data of FACS-sorted neurons to enrich for genes specifically expressed in NBs. The projects of this application analyse different genes from the transcriptome data in detail to study their function in NB self-renewal and NB lineage identity determination to characterize individual NB lineages in the larval CNS.