The identification of fundamental molecular regulators of ribosomal DNA and nucleolar organization in fission yeast
裂殖酵母核糖体 DNA 和核仁组织基本分子调节因子的鉴定
基本信息
- 批准号:10154642
- 负责人:
- 金额:$ 3.45万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-01-03 至 2024-01-02
- 项目状态:已结题
- 来源:
- 关键词:AddressArchitectureAreaAutomobile DrivingBacterial DNABiogenesisBiological ModelsCell CycleCell Cycle ProgressionCell Cycle RegulationCell NucleolusCell physiologyCellsCellular biologyChromosome StructuresChromosomesDNADNA analysisDNA-Binding ProteinsExhibitsFission YeastFluorescence MicroscopyFutureGeneticGenetic TranscriptionGenomeGoalsHumanImageImage AnalysisImaging DeviceInterphaseKnowledgeLabelMaintenanceMalignant NeoplasmsMeasuresMethodsModelingMolecularMolecular BiologyMolecular Biology TechniquesMolecular and Cellular BiologyMorphologyNeurodegenerative DisordersNuclearNucleolar Organizer RegionNucleolar ProteinsOrganellesOrganismPhysiologic pulseProcessProductionProtein BiosynthesisProteinsQuantitative MicroscopyRNARNA ProcessingResearchRibosomal DNARibosomal ProteinsRibosomal RNARibosomesRoleSaccharomycetalesStructureTestingTranscriptVariantcell growthdeletion libraryfield studygenetic approachgenetic manipulationgenome-widehigh throughput analysishuman diseaseimaging geneticsinnovationmutantnovelnovel strategiesnucleolar organizing regionprotein complextool
项目摘要
Project Abstract
Ribosome production relies on a nuclear organelle called the nucleolus. Within this structure, ribosomal DNA
(rDNA) is transcribed to form RNA transcripts that associate with ribosomal proteins. Nucleolar architecture is
altered in many human diseases including numerous cancers, prompting several studies to search for
regulators of nucleolar morphology. While these studies rely on analysis of nucleolar proteins or ribosome
production to identify molecular regulators, few studies have defined cell cycle-specific mechanisms for
regulating nucleolar structure. Furthermore, no studies have examined the impact of rDNA spatial organization
on nucleolar morphology despite rDNA loci’s known role as Nucleolar Organizer Regions. The long-term goal
is to understand conserved regulatory mechanisms of rDNA and nucleolar organization. The overall objectives
of this proposal are to (i) to identify molecular regulators of rDNA spatial organization and (ii) define the
processes driving cell cycle-regulated nucleolar morphology. The central hypothesis is that the spatial
organization of rDNA is regulated, in part, by chromosome organizing proteins and ribosome biogenesis;
furthermore, these ribosome biogenesis processes are cell cycle-regulated, driving dynamic reorganization of
nucleolar morphology during interphase. The rationale for this study is that identification of conserved
molecular regulators of rDNA and nucleolar organization in fission yeast will provide a template for future
research in higher organisms. This central hypothesis will be tested by two specific aims: 1) identify molecular
regulators of rDNA spatial organization in fission yeast; and 2) define the processes driving cell cycle-regulated
nucleolar morphology. For aim 1, a novel tool for analysis of rDNA spatial organization in live cells has been
developed in fission yeast. This tool will be used to quantify rDNA spatial organization by fluorescence
microscopy in candidate mutants with altered chromosome organization and DNA topology factors. This
analysis will be expanded by a genome-wide high-throughput imaging screen to broadly identify regulators of
rDNA spatial organization. Aim 2 will apply fluorescence microscopy, cell biology, and molecular biology
approaches to examine the role of cell cycle-regulated ribosome biogenesis factors in interphase nucleolar
morphology. These studies examine rDNA and nucleolar morphology in fission yeast, a model system notable
for its application to higher organisms and ease of genetic manipulation. To understand the relationship
between nucleolar morphology and human disease, the regulatory mechanisms behind rDNA and nucleolar
organization must be identified. This study applies innovative imaging tools with advanced cellular and
molecular biology techniques to broadly identify fundamental molecular regulators of rDNA and nucleolar
morphology, providing a framework for future studies in human cells.
项目摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Alexandria Jane Cockrell其他文献
Alexandria Jane Cockrell的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Alexandria Jane Cockrell', 18)}}的其他基金
Lexy Cockrell Childcare Supplement
莱克西·科克雷尔 (Lexy Cockrell) 儿童保育补充剂
- 批准号:
10596408 - 财政年份:2022
- 资助金额:
$ 3.45万 - 项目类别:
The identification of fundamental molecular regulators of ribosomal DNA and nucleolar organization in fission yeast
裂殖酵母核糖体 DNA 和核仁组织基本分子调节因子的鉴定
- 批准号:
10335148 - 财政年份:2021
- 资助金额:
$ 3.45万 - 项目类别:
The identification of fundamental molecular regulators of ribosomal DNA and nucleolar organization in fission yeast
裂殖酵母核糖体 DNA 和核仁组织基本分子调节因子的鉴定
- 批准号:
10845906 - 财政年份:2021
- 资助金额:
$ 3.45万 - 项目类别:
The identification of fundamental molecular regulators of ribosomal DNA and nucleolar organization in fission yeast
裂殖酵母核糖体 DNA 和核仁组织基本分子调节因子的鉴定
- 批准号:
10543180 - 财政年份:2021
- 资助金额:
$ 3.45万 - 项目类别:
相似海外基金
Practical Study on Disaster Countermeasure Architecture Model by Sustainable Design in Asian Flood Area
亚洲洪泛区可持续设计防灾建筑模型实践研究
- 批准号:
17K00727 - 财政年份:2017
- 资助金额:
$ 3.45万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional architecture of a face processing area in the common marmoset
普通狨猴面部处理区域的功能架构
- 批准号:
9764503 - 财政年份:2016
- 资助金额:
$ 3.45万 - 项目类别:
SBIR Phase II: Area and Energy Efficient Error Floor Free Low-Density Parity-Check Codes Decoder Architecture for Flash Based Storage
SBIR 第二阶段:用于基于闪存的存储的面积和能源效率高、无错误层的低密度奇偶校验码解码器架构
- 批准号:
1632562 - 财政年份:2016
- 资助金额:
$ 3.45万 - 项目类别:
Standard Grant
Heating and airconditioning by hypocausts in residential and representative architecture in Rome and Latium studies of a phenomenon of luxury in a favoured climatic area of the Roman Empire on the basis of selected examples.
罗马和拉齐奥的住宅和代表性建筑中的火烧供暖和空调根据选定的例子,研究了罗马帝国有利的气候地区的奢华现象。
- 批准号:
317469425 - 财政年份:2016
- 资助金额:
$ 3.45万 - 项目类别:
Research Grants
SBIR Phase I: Area and Energy Efficient Error Floor Free Low-Density Parity-Check Codes Decoder Architecture for Flash Based Storage
SBIR 第一阶段:用于基于闪存的存储的面积和能源效率高、无错误层低密度奇偶校验码解码器架构
- 批准号:
1520137 - 财政年份:2015
- 资助金额:
$ 3.45万 - 项目类别:
Standard Grant
A Study on The Spatial Setting and The Inhavitant's of The Flood Prevention Architecture in The Flood Area
洪泛区防洪建筑空间设置及居民生活研究
- 批准号:
26420620 - 财政年份:2014
- 资助金额:
$ 3.45万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Area and power efficient interconnect architecture for multi-bit processing on FPGAs
用于 FPGA 上多位处理的面积和功率高效互连架构
- 批准号:
327691-2007 - 财政年份:2011
- 资助金额:
$ 3.45万 - 项目类别:
Discovery Grants Program - Individual
A FUNDAMENTAL STUDY ON UTILIZATION OF THE POST-WAR ARCHITECTURE AS URBAN REGENERATION METHOD, A case of the central area of Osaka city
战后建筑作为城市更新方法的基础研究——以大阪市中心区为例
- 批准号:
22760469 - 财政年份:2010
- 资助金额:
$ 3.45万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Area and power efficient interconnect architecture for multi-bit processing on FPGAs
用于 FPGA 上多位处理的面积和功率高效互连架构
- 批准号:
327691-2007 - 财政年份:2010
- 资助金额:
$ 3.45万 - 项目类别:
Discovery Grants Program - Individual
Area and power efficient interconnect architecture for multi-bit processing on FPGAs
用于 FPGA 上多位处理的面积和功率高效互连架构
- 批准号:
327691-2007 - 财政年份:2009
- 资助金额:
$ 3.45万 - 项目类别:
Discovery Grants Program - Individual














{{item.name}}会员




