MRI: Acquisition of a Fluidigm BioMark HD system for research and training at Humboldt State University

MRI：洪堡州立大学购买 Fluidigm BioMark HD 系统用于研究和培训

• 批准号: 1531910
• 负责人: Andrew Kinziger
• 金额: $ 45.71万
• 依托单位: Humboldt State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531910&HistoricalAwards=false
• 关键词: MRI; Acquisition; Fluidigm; BioMark; HD

An award is made to Humboldt State University (HSU) to acquire a Fluidigm Juno/Biomark system. The Juno/Biomark consists of a real-time Polymerase Chain Reaction (PCR) instrument and a controller designed to load a variety of microfluidic arrays. PCR is a fast method of copying small segments of DNA critical to molecular and genetic analysis. The instrument supports a diverse set of applications, including gene expression, SNP genotyping, mutant detection, and absolute quantification of nucleic-acid sequences utilizing digital PCR. The key feature of the instrumentation are microfluidic arrays that allow thousands of nanoliter reactions to be run in parallel. Several different microfluidic arrays are available, thus the instrument can support users with low as well as high-throughput needs. The instrument will be housed in the College of Natural Resources and Sciences Core Facility, a shared-use laboratory for teaching and research. The Juno/Biomark will serve as a centerpiece for training undergraduate and graduate students in the sciences, including those in the Stem Cell Biology training program and laboratory sections of Behavioral Neuroscience, Cellular Neuroscience, Conservation Genetics, Genetics, and Animal Physiology. Since HSU is a Hispanic Serving Institution, and has a high concentration of Native American students, this project will enable education and training of underrepresented groups with advanced genetic techniques. Collaboration with local teachers and their students at Hoopa High School (in the Hoopa Tribal Nation), McKinleyville High School, and the Redwood Science Institute, an institute which provides training for high school teachers, will provide opportunities for high school students to use this cutting-edge instrument.The Juno/Biomark system will be used by a broad range of researchers across multiple departments and scientific disciplines (Biology, Fisheries, Wildlife, and Psychology) at HSU. The hallmark of the instrumentation is its capacity to support a diversity of applications and its versatility to accommodate different throughput needs, including high-throughput options. Researchers Andrew Kinziger, Sean Craig, Ehtan Gahtan, Micaela Szykman Gunther, Brian Tissot, Darren Ward, Margaret Wilzbach, Jianmin Zhong and colleagues will use the instrumentation to conduct investigations in the areas of conservation and management of fishes, ecology and evolution of invasive species, behavioral neuroscience, behavior and social interactions of mammals, conservation ecology of marine fishes and invertebrates, and detection and control of tick-borne diseases. The new instrumentation will enhance existing research programs and allow investigations that are essentially impossible with existing equipment. The Juno/Biomark system will substantially improve research infrastructure at HSU and the region by providing access to high throughput genetic capabilities to a rural area of northern California far from other universities with comparable instrumentation.

洪堡州立大学(HSU)获得了一套Fluidigm Juno/BioMark系统。Juno/BioMark由一个实时聚合酶链式反应(PCR)仪器和一个控制器组成，该控制器旨在加载各种微流控阵列。聚合酶链式反应是一种复制小片段DNA的快速方法，对分子和遗传分析至关重要。该仪器支持多种应用，包括基因表达、SNP基因分型、突变检测以及利用数字聚合酶链式反应对核酸序列进行绝对量化。该仪器的关键特征是微流控阵列，允许数千纳升反应并行运行。有几种不同的微流控阵列可供选择，因此该仪器既可以支持高通量需求，也可以支持低通量需求。该仪器将安置在自然资源与科学学院的核心设施中，这是一个教学和研究的共用实验室。Juno/BioMark将作为培训本科生和研究生的核心科学，包括干细胞生物学培训计划和行为神经科学、细胞神经科学、保护遗传学、遗传学和动物生理学的实验室部分。由于徐州大学是一个西班牙裔服务机构，美洲原住民学生高度集中，这个项目将使教育和培训代表不足的群体与先进的基因技术。与霍帕高中(霍帕部落民族)、麦金利维尔高中和红木科学研究所(一家为高中教师提供培训的机构)的当地教师和学生的合作，将为高中生提供使用这种尖端工具的机会。朱诺/生物标记系统将被华中大学多个系和科学学科(生物学、渔业、野生动物和心理学)的广泛研究人员使用。该工具的特点是其支持多种应用程序的能力以及适应不同吞吐量需求(包括高吞吐量选项)的多功能性。研究人员Andrew Kinziger、Sean Craig、Ehtan Gahtan、Micaela Szykman Gunther、Brian Tissot、Darren Ward、玛格丽特·威尔兹巴赫、钟建民及其同事将使用该仪器在鱼类保护和管理、入侵物种的生态和进化、行为神经科学、哺乳动物的行为和社会互动、海洋鱼类和无脊椎动物的保护生态以及扁虱传播疾病的检测和控制等领域进行调查。新的仪器将增强现有的研究计划，并允许进行用现有设备基本上不可能进行的调查。Juno/BioMark系统将为加州北部远离其他拥有类似仪器的大学的农村地区提供获得高通量基因能力的途径，从而极大地改善HSU和该地区的研究基础设施。