MRI: Acquisition of a Fluidigm BioMark HD system for research and training at Humboldt State University

MRI：洪堡州立大学购买 Fluidigm BioMark HD 系统用于研究和培训

• 批准号: 1531910
• 负责人: Andrew Kinziger
• 金额: $ 45.71万
• 依托单位: Humboldt State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531910&HistoricalAwards=false
• 关键词: MRI; Acquisition; Fluidigm; BioMark; HD

An award is made to Humboldt State University (HSU) to acquire a Fluidigm Juno/Biomark system. The Juno/Biomark consists of a real-time Polymerase Chain Reaction (PCR) instrument and a controller designed to load a variety of microfluidic arrays. PCR is a fast method of copying small segments of DNA critical to molecular and genetic analysis. The instrument supports a diverse set of applications, including gene expression, SNP genotyping, mutant detection, and absolute quantification of nucleic-acid sequences utilizing digital PCR. The key feature of the instrumentation are microfluidic arrays that allow thousands of nanoliter reactions to be run in parallel. Several different microfluidic arrays are available, thus the instrument can support users with low as well as high-throughput needs. The instrument will be housed in the College of Natural Resources and Sciences Core Facility, a shared-use laboratory for teaching and research. The Juno/Biomark will serve as a centerpiece for training undergraduate and graduate students in the sciences, including those in the Stem Cell Biology training program and laboratory sections of Behavioral Neuroscience, Cellular Neuroscience, Conservation Genetics, Genetics, and Animal Physiology. Since HSU is a Hispanic Serving Institution, and has a high concentration of Native American students, this project will enable education and training of underrepresented groups with advanced genetic techniques. Collaboration with local teachers and their students at Hoopa High School (in the Hoopa Tribal Nation), McKinleyville High School, and the Redwood Science Institute, an institute which provides training for high school teachers, will provide opportunities for high school students to use this cutting-edge instrument.The Juno/Biomark system will be used by a broad range of researchers across multiple departments and scientific disciplines (Biology, Fisheries, Wildlife, and Psychology) at HSU. The hallmark of the instrumentation is its capacity to support a diversity of applications and its versatility to accommodate different throughput needs, including high-throughput options. Researchers Andrew Kinziger, Sean Craig, Ehtan Gahtan, Micaela Szykman Gunther, Brian Tissot, Darren Ward, Margaret Wilzbach, Jianmin Zhong and colleagues will use the instrumentation to conduct investigations in the areas of conservation and management of fishes, ecology and evolution of invasive species, behavioral neuroscience, behavior and social interactions of mammals, conservation ecology of marine fishes and invertebrates, and detection and control of tick-borne diseases. The new instrumentation will enhance existing research programs and allow investigations that are essentially impossible with existing equipment. The Juno/Biomark system will substantially improve research infrastructure at HSU and the region by providing access to high throughput genetic capabilities to a rural area of northern California far from other universities with comparable instrumentation.

洪堡州立大学（HSU）获得Fluidigm Juno/Biomark系统。Juno/Biomark由一个实时聚合酶链反应（PCR）仪器和一个用于加载各种微流体阵列的控制器组成。PCR是一种快速复制DNA小片段的方法，对分子和遗传分析至关重要。该仪器支持多种应用，包括基因表达，SNP基因分型，突变检测，以及利用数字PCR对核酸序列进行绝对定量。该仪器的主要特点是微流体阵列，允许数千纳升的反应并行运行。几种不同的微流体阵列可用，因此该仪器可以支持低通量和高通量需求的用户。该仪器将被安置在自然资源与科学学院的核心设施中，这是一个用于教学和研究的共享实验室。Juno/Biomark将作为本科生和研究生科学培训的中心，包括干细胞生物学培训计划和行为神经科学、细胞神经科学、保护遗传学、遗传学和动物生理学的实验室部分。由于HSU是一所西班牙裔服务机构，并且美洲原住民学生高度集中，因此该项目将能够通过先进的遗传技术对代表性不足的群体进行教育和培训。与Hoopa高中（在Hoopa部落国家）、McKinleyville高中和为高中教师提供培训的红木科学研究所的当地教师和学生合作，将为高中学生提供使用这种尖端仪器的机会。Juno/Biomark系统将被HSU多个部门和科学学科（生物学，渔业，野生动物和心理学）的广泛研究人员使用。该仪器的特点是能够支持多种应用程序，并具有适应不同吞吐量需求的通用性，包括高吞吐量选项。研究人员Andrew Kinziger， Sean Craig, Ehtan Gahtan, Micaela Szykman Gunther, Brian Tissot, Darren Ward, Margaret Wilzbach， Jianmin Zhong及其同事将使用该仪器在鱼类保护和管理，入侵物种生态学和进化，行为神经科学，哺乳动物行为和社会互动，海洋鱼类和无脊椎动物保护生态学以及蜱传疾病的检测和控制等领域进行调查。新的仪器将加强现有的研究计划，并允许现有设备基本上不可能进行的调查。Juno/Biomark系统将极大地改善HSU和该地区的研究基础设施，为加州北部偏远地区提供高通量遗传能力，远离其他拥有类似仪器的大学。