MRI: Acquisition of a Fluidigm BioMark HD system for research and training at Humboldt State University

洪堡州立大学（HSU）获得了一项奖励，以获得Fluidigm Juno/Biomark系统。 Juno/Biomark由实时聚合酶链式反应（PCR）仪器和控制器组成，该控制器旨在加载各种微流体阵列。PCR是一种快速复制小片段DNA的方法，对分子和遗传分析至关重要。该仪器支持多种应用，包括基因表达、SNP基因分型、突变体检测和利用数字PCR对核酸序列进行绝对定量。该仪器的关键特征是微流体阵列，允许并行运行数千纳升的反应。 有几种不同的微流控阵列可用，因此该仪器可以支持具有低通量和高通量需求的用户。 该仪器将被安置在自然资源和科学学院的核心设施，一个共享使用的教学和研究实验室。 Juno/Biomark将作为培养科学本科生和研究生的核心，包括干细胞生物学培训计划和行为神经科学，细胞神经科学，保护遗传学，遗传学和动物生理学的实验室部分。由于HSU是一个西班牙裔服务机构，并有一个高度集中的美洲原住民学生，这个项目将使教育和培训的代表性不足的群体与先进的遗传技术。 与Hoopa高中的当地教师和学生合作（在Hoopa部落国家），麦金莱维尔高中和红木科学研究所，一个为高中教师提供培训的机构，将为高中学生提供使用这种尖端仪器的机会。Juno/Biomark系统将被多个部门和科学学科的广泛研究人员使用（生物学，渔业，野生动物和心理学）在HSU。 该仪器的特点是其支持多种应用的能力和适应不同吞吐量需求的多功能性，包括高吞吐量选项。 研究人员Andrew Kinziger，Sean克雷格，Ehtan Gahtan，Micaela Szykman Gunther，Brian Tissot，Darren Ward，Margaret Wilzbach，Jianmin Zhong及其同事将使用该仪器进行鱼类保护和管理，入侵物种生态学和进化，行为神经科学，哺乳动物行为和社会相互作用，海洋鱼类和无脊椎动物保护生态学，以及蜱传疾病的检测和控制。 新的仪器将加强现有的研究计划，并允许调查，基本上是不可能的现有设备。 朱诺/生物标记系统将大大改善研究基础设施在HSU和该地区提供访问高通量遗传能力的北方加州农村地区远离其他大学具有可比的仪器。

An award is made to Humboldt State University (HSU) to acquire a Fluidigm Juno/Biomark system. The Juno/Biomark consists of a real-time Polymerase Chain Reaction (PCR) instrument and a controller designed to load a variety of microfluidic arrays. PCR is a fast method of copying small segments of DNA critical to molecular and genetic analysis. The instrument supports a diverse set of applications, including gene expression, SNP genotyping, mutant detection, and absolute quantification of nucleic-acid sequences utilizing digital PCR. The key feature of the instrumentation are microfluidic arrays that allow thousands of nanoliter reactions to be run in parallel. Several different microfluidic arrays are available, thus the instrument can support users with low as well as high-throughput needs. The instrument will be housed in the College of Natural Resources and Sciences Core Facility, a shared-use laboratory for teaching and research. The Juno/Biomark will serve as a centerpiece for training undergraduate and graduate students in the sciences, including those in the Stem Cell Biology training program and laboratory sections of Behavioral Neuroscience, Cellular Neuroscience, Conservation Genetics, Genetics, and Animal Physiology. Since HSU is a Hispanic Serving Institution, and has a high concentration of Native American students, this project will enable education and training of underrepresented groups with advanced genetic techniques. Collaboration with local teachers and their students at Hoopa High School (in the Hoopa Tribal Nation), McKinleyville High School, and the Redwood Science Institute, an institute which provides training for high school teachers, will provide opportunities for high school students to use this cutting-edge instrument.The Juno/Biomark system will be used by a broad range of researchers across multiple departments and scientific disciplines (Biology, Fisheries, Wildlife, and Psychology) at HSU. The hallmark of the instrumentation is its capacity to support a diversity of applications and its versatility to accommodate different throughput needs, including high-throughput options. Researchers Andrew Kinziger, Sean Craig, Ehtan Gahtan, Micaela Szykman Gunther, Brian Tissot, Darren Ward, Margaret Wilzbach, Jianmin Zhong and colleagues will use the instrumentation to conduct investigations in the areas of conservation and management of fishes, ecology and evolution of invasive species, behavioral neuroscience, behavior and social interactions of mammals, conservation ecology of marine fishes and invertebrates, and detection and control of tick-borne diseases. The new instrumentation will enhance existing research programs and allow investigations that are essentially impossible with existing equipment. The Juno/Biomark system will substantially improve research infrastructure at HSU and the region by providing access to high throughput genetic capabilities to a rural area of northern California far from other universities with comparable instrumentation.