Understanding synaptopathy at inner hair cell ribbon synapses on the ultrastructural level (A07)

在超微结构水平上了解内毛细胞带突触的突触病 (A07)

• 批准号: 221819943
• 金额: --
• 依托单位: Institut für Auditorische Neurowissenschaften
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/221819943?language=en
• 关键词: Understanding; synaptopathy; inner; hair; cell

Defects in auditory synaptic transmission cause hearing impairment, and a degeneration of ribbon synapses takes place during age-related hearing loss. Understanding different phases of exocytosis at inner hair cell (IHC) ribbon synapses and determining structural changes at IHC active zones that precede the loss of ribbons and postsynaptic spiral ganglion neurons during age-related ribbon degeneration are the two aims of this study. We will combine optogenetics with high-pressure freezing and electron tomography on different mutants of presynaptic proteins and will perform large volume 3D-ultrastructural reconstructions of IHCs in distinct stages of aging together with electron-tomography.

听觉突触传递的缺陷导致听力损伤，带状突触的退化发生在与年龄相关的听力损失期间。本研究的两个目的是了解内毛细胞（IHC）带状突触胞吐的不同阶段，并确定在年龄相关的带状变性过程中带状和突触后螺旋神经节神经元损失之前的IHC活性区的结构变化。我们将结合联合收割机光遗传学与高压冷冻和电子断层扫描对不同的突变体的突触前蛋白，并将进行大量的三维超微结构重建的IHC在不同阶段的老化与电子断层扫描。