Characterization of mast cell anatomy and function in primate airways - interaction with the nervous system

灵长类气道肥大细胞解剖学和功能的表征 - 与神经系统的相互作用

• 批准号: 221967554
• 负责人: Professor Dr. Armin Braun
• 金额: --
• 依托单位: Fraunhofer-Institut für Toxikologie und Experimentelle Medizin (ITEM)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/221967554?language=en
• 关键词: Characterization; mast; cell; anatomy; function

This project aims to investigate mast cell biology in primate airways where the phylogenetic homology to the human respiratory tract is very high. For this purpose it will focus on airways of the nonhuman primate Callithrix jacchus (common marmoset). In our previous work we could show that the mechanisms of mast cell induced bronchoconstriction are similar between marmosets and humans in contrast to laboratory rodents. The project includes three major parts. 1. Comparative anatomy of airway mast cells with special emphasis on mast cell-nerve interaction using histological and immunohistochemical methods, electron microscopy (EM), as well as confocal microscopy. 2. Functional characterization of mast cell function in the mechanisms of bronchoconstriction ex vivo in precision cut lung slices (PCLS). Videomicroscopy and two-photon- microscopy following pharmacological intervention with e. g. neuropeptide antagonists will allow insights in the mechanisms of mast cell-nerve interaction. The results will be confirmed in human PCLS. 3. Finally, in vivo lung function measurements in the marmoset will allow confirming the function of mast cell-nerve interaction in the induction of bronchoconstriction.

本计画的目的是研究灵长类动物呼吸道中肥大细胞的生物学特性，因为灵长类动物的呼吸道与人类呼吸道的系统发育同源性非常高。为此，它将集中在非人类灵长类Callithrix jacchus（普通绒猴）的气道。在我们以前的工作中，我们可以表明，肥大细胞诱导支气管收缩的机制是相似的绒猴和人类相比，实验室啮齿动物。该项目包括三个主要部分。1.气道肥大细胞的比较解剖学，特别强调肥大细胞-神经的相互作用，使用组织学和免疫组织化学方法，电子显微镜（EM），以及共聚焦显微镜。2.肥大细胞在精密切割肺切片（PCLS）中离体支气管收缩机制中的功能表征。药物干预后的视频显微镜和双光子显微镜。G.神经肽拮抗剂将允许了解肥大细胞-神经相互作用的机制。将在人PCLS中确认结果。3.最后，在体内肺功能的绒猴测量将允许确认肥大细胞神经相互作用的支气管收缩的诱导功能。