IDBR: TYPE A - Isomotive dielectrophoresis for enhanced analyses of cell subpopulations

IDBR：A 型 - 用于增强细胞亚群分析的同动力介电泳

• 批准号: 1550509
• 负责人: Stuart Williams
• 金额: $ 37.25万
• 依托单位: University of Louisville Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1550509&HistoricalAwards=false
• 关键词: IDBR; TYPE; Isomotive; dielectrophoresis; enhanced

An award is made to the University of Louisville to develop an isomotive dielectrophoresis (isoDEP) microfluidic device that will extract dielectric properties of individual cells. IsoDEP can be used in applications where cell identification and analysis is critical such as studying phytoplankton populations for environmental monitoring, bacteria identification for monitoring food safety, or identifying cancerous cells. Details of the isoDEP device will be disseminated to the local biological community and the general public through technical presentations and social media. Educational outreach will include the exposure of isoDEP physics and instrument development to undergraduate students from rural impoverished regions of Appalachia and to high school students from Central High School, one of the oldest urban secondary schools in Kentucky.The analysis of individual cells within a population can monitor environmental conditions or assess human health. In particular, dielectric properties provide insight into cell conditions and can be used to identify and characterize cell sub-populations. For example, cell properties such as membrane capacitance, membrane resistance, and cytoplasmic conductivity directly reflect the cell's biological structure. This project will extract dielectric measurements through particle tracking under an electrokinetic environment at a rate of at least one order of magnitude greater than current methods. Through collaboration with Murray State University, freshwater phytoplankton analysis will be conducted for environmental monitoring. In collaboration with a local small business (Ikotech, LLC), an additional objective of this research is to develop an isoDEP microfluidic instrument to be used by scientists worldwide.

路易斯维尔大学被授予一项奖项，以开发一种将提取单个细胞的介电性质的等摩尔介质电泳微流控设备。IsoDEP可用于细胞鉴定和分析至关重要的应用，如用于环境监测的浮游植物种群研究、用于监测食品安全的细菌鉴定或鉴定癌细胞。将通过技术介绍和社交媒体向当地生物界和公众传播有关isdep装置的详细信息。教育外展将包括向来自阿巴拉契亚农村贫困地区的本科生和肯塔基州最古老的城市中学之一中央高中的高中生介绍isdep物理和仪器开发。对人口中单个细胞的分析可以监测环境条件或评估人类健康。具体地说，介电特性提供了对细胞条件的洞察，并可用于识别和表征细胞亚群。例如，膜电容、膜电阻和细胞质电导率等细胞属性直接反映了细胞的生物结构。该项目将在电动环境下通过粒子跟踪提取介电测量，速度至少比目前的方法高一个数量级。通过与默里州立大学的合作，将进行淡水浮游植物分析，以进行环境监测。与当地一家小型企业(Ikotech，LLC)合作，这项研究的另一个目标是开发一种供世界各地科学家使用的isdep微流控仪器。