Moonlighting from sugar to metal

本项目拟利用综合遗传学、生物化学和组学来破译高度保守的OST3蛋白的作用，该蛋白涉及调节蛋白质糖基化和运输镁的不同功能。该项目计划详细描述OST3蛋白在调节哺乳动物糖基化中的作用，并重建OST3蛋白与底物相互作用的脊椎动物共同进化轨迹。它还旨在确定和表征OST3蛋白介导的镁转运的调节、机制和代谢后果。这些结果可能提供真核生物学的见解，并使糖蛋白生产和调节细胞代谢的工程系统取得进展，具有潜在的研究和治疗用途。

This project intends to use integrated genetics, biochemistry and omics to decipher the roles of the highly conserved OST3 proteins, which have been implicated in the disparate functions of regulating protein glycosylation and transporting magnesium. The project plans to detail the role of OST3 proteins in regulating mammalian glycosylation and reconstruct the vertebrate co-evolutionary trajectory of OST3 protein–substrate interactions. It also aims to identify and characterise the regulation, mechanisms and metabolic consequences of OST3 protein-mediated magnesium transport. These outcomes may provide insights into eukaryotic biology, and allow advances in engineered systems for glycoprotein production and modulating cellular metabolism with potential research and therapeutic utility.