Collaborative Research: Persistence, entrainment, and function of circadian rhythms in arctic ground squirrels

合作研究：北极地松鼠昼夜节律的持续性、夹带和功能

• 批准号: 1602126
• 负责人: Charles Buck
• 金额: $ 32.7万
• 依托单位: Northern Arizona University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1602126&HistoricalAwards=false
• 关键词: Collaborative; Research; Persistence; entrainment; function

The Earth's light-dark (LD) cycle is the strongest cue for entraining circadian rhythms and is considered the primary driver for the emergence and evolution of endogenous clocks. In polar regions, however, photoperiod exhibits extreme annual variation culminating with the sun remaining above or below the horizon for extended periods. Without a defined LD cycle, some arctic residents lose daily organization of behavior and physiology, and it has been hypothesized that the molecular clockwork that drives circadian rhythms may be weak or absent in arctic vertebrates. This study tests the hypothesis that persistence of circadian organization in some arctic mammals is adaptive because it allows organisms to minimize thermoregulatory costs. Specifically, the investigators will examine persistence, entrainment, and function of circadian rhythms in the arctic ground squirrel (AGS) during the continuous daylight of the active season and the continuous dark of hibernation. Four research objectives span from molecular and neurobiological mechanisms to physiological, behavioral and ecological adaptation: (1) determine when circadian rhythms are exhibited during the annual cycle of free-living AGS and establish if the onset of rhythmicity in spring coincides with first exposure to light, (2) determine if circadian rhythms persist in the master clock of AGS during hibernation by measuring patterns of clock gene expression in the suprachiasmatic nuclei (SCN) of the brain, (3) determine if AGS entrain circadian rhythms to daily changes in light quality or intensity, and (4) investigate patterns of re-entrainment and the function of rhythms in the arctic summer by experimentally phase-shifting free-living ground squirrels and measuring metabolic costs of nocturnal activity. Whether circadian rhythms persist during hibernation is contentious. Transcription and translation are thought to be globally suppressed during deep torpor and the only study to examine clock-gene expression within the SCN during multi-day torpor found no evidence of oscillations. However, circadian body temperature rhythms have been observed in some studies of captive ground squirrels during torpor and timing of arousals has been hypothesized to be controlled by a circadian clock. Persistence of oscillations in clock-gene expression in the SCN would support existence of tissue-specific mechanisms for translational control of a subset of genes relevant to survival during hibernation. In contrast, expression of daily rhythms may not be compatible with hibernation; SCN function and or output may be inhibited to prolong torpor. This study integrates research and learning by (1) training post-docs and students from three universities in ecophysiological studies in the laboratory and field, (2) inclusion of Alaska Natives in research through participation in the Alaska Native Science and Engineering Program, and (3) increasing K-12 students' engagement in research by participating in Teacher Research Experience programs. Results of research will be disseminated locally in classroom presentations and nationally through the University of Alaska media relations team.

地球的光暗（LD）周期是诱导昼夜节律的最强线索，被认为是内源性时钟出现和进化的主要驱动力。然而，在极地地区，光周期表现出极端的年度变化，最终太阳在很长一段时间内保持在地平线以上或以下。如果没有一个明确的LD周期，一些北极居民失去了日常组织的行为和生理，它已被假设，驱动昼夜节律的分子时钟可能是弱或缺乏在北极脊椎动物。这项研究测试的假设，在一些北极哺乳动物的昼夜节律组织的持久性是适应性的，因为它允许生物体，以尽量减少温度调节成本。具体来说，研究人员将研究北极地松鼠（AGS）在活动季节的连续日光和冬眠的连续黑暗期间的昼夜节律的持续性，夹带和功能。四个研究目标涵盖从分子和神经生物学机制到生理，行为和生态适应：（1）确定在自由生活的AGS的年周期期间何时表现出昼夜节律，并确定春季节律性的开始是否与第一次暴露于光一致，（2）通过测量视交叉上核（SCN）中时钟基因表达模式来确定AGS主时钟在冬眠期间是否持续昼夜节律（3）确定AGS是否将昼夜节律带入光质量或强度的日常变化中，（4）通过实验性相移自由生活的地松鼠并测量夜间活动的代谢成本来研究北极夏季的再带入模式和节律功能。在冬眠期间昼夜节律是否持续存在是有争议的。转录和翻译被认为在深度休眠期间受到全面抑制，并且在多日休眠期间检查SCN内时钟基因表达的唯一研究没有发现振荡的证据。然而，在一些研究中观察到的昼夜体温节律的圈养地松鼠在麻痹和觉醒的时间已被假设为由生物钟控制。持续振荡的SCN中的时钟基因表达将支持存在的组织特异性机制的翻译控制的一个子集的基因在冬眠期间的生存。相反，日常节律的表达可能与冬眠不相容; SCN功能和/或输出可能被抑制以延长麻痹。本研究通过以下方式整合研究和学习：（1）在实验室和现场培训来自三所大学的博士后和学生进行生态生理学研究，（2）通过参与阿拉斯加土著科学与工程计划将阿拉斯加土著纳入研究，以及（3）通过参与教师研究体验计划增加K-12学生参与研究。研究结果将通过阿拉斯加大学媒体关系团队在当地的课堂演示和全国范围内传播。