RUI: Evaporation-driven Capillary Flow in Paper-Based Microfluidic Devices

RUI：纸基微流体装置中蒸发驱动的毛细管流

• 批准号: 1605499
• 负责人: Andres Martinez
• 金额: $ 18.14万
• 依托单位: California Polytechnic State University Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2022-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1605499&HistoricalAwards=false
• 关键词: RUI; Evaporation; driven; Capillary; Flow

PI: Martinez, AndresProposal Number: 1605499The goal of the proposed research is to study fluid flow through the capillaries in microPADS (a special type of paper that can be used for analytic purposes in bio-related diagnostics in areas where complex hardware is not available.) A model for the process is proposed to be developed in collaboration with researchers at Princeton University.The proposed work focuses on the experimental study and modeling of evaporation-driven capillary flow (ECF) through paper-based microfluidic devices (microPADs) in order to use this type of fluid transport for the development of paper-based diagnostic devices capable of performing automated multi-step assays. The proposed work will result in an investigation of ECF in microPADs with infinite and finite fluid reservoirs; an investigation of the effects of the design of the microPAD on ECF; the development of mathematical models to describe ECF in microPADs, and the application of these models toward the design and development of prototype microPADs for conducting automated multi-step diagnostic assays, including an enzyme inhibition assay and an immuno-chromatographic assay with signal amplification. All the experimental work will be conducted by undergraduate student using relatively simple materials and fabrication methods. The proposed work will also serve to advance the general understanding of ECF, with implications for other systems such as plants and plasticbased microfluidic devices. The work with prototype diagnostic devices will elucidate mechanisms for developing more sophisticated point-of-care diagnostic assays that are still very simple to use. Finally, the project will enable the development of new assays that require at least two, timed, sequential steps, and that were previously not possible due to limitations with the current capabilities of microPADs.

主要研究者：Martinez，AndresProposal编号：1605499拟议研究的目标是研究通过microPADS（一种特殊类型的纸张，可用于生物相关诊断领域的分析目的，复杂的硬件不可用）中毛细管的流体流动。该过程的模型拟与普林斯顿大学的研究人员合作开发。拟议的工作重点是通过纸基微流体装置（microPAD）的蒸发驱动毛细流动（ECF）的实验研究和建模，以便使用这种类型的流体传输用于开发能够执行自动化多步骤分析的纸基诊断装置。本论文的主要工作包括：研究无限大和有限大两种贮液器的微PAD的ECF;研究微PAD设计对ECF的影响;描述microPAD中ECF的数学模型的开发，以及这些模型在设计和开发用于进行自动化多步诊断测定的原型microPAD中的应用，包括酶抑制测定和具有信号放大的免疫层析测定。所有的实验工作将由本科生使用相对简单的材料和制造方法进行。拟议的工作也将有助于推进对ECF的普遍理解，并对其他系统（如植物和塑料微流体装置）产生影响。原型诊断设备的工作将阐明开发更复杂的即时诊断检测的机制，这些检测仍然非常简单易用。最后，该项目将能够开发新的检测方法，这些方法需要至少两个定时的连续步骤，并且由于microPAD当前功能的限制，以前是不可能的。