Regulation of the DEAD-box RNA helicase Sub2 by TREX (A07)

TREX (A07) 对 DEAD-box RNA 解旋酶 Sub2 的调节

• 批准号: 224214760
• 金额: --
• 依托单位: Max-Planck-Institut für Biochemie (MPIB)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/224214760?language=en
• 关键词: Regulation; DEAD; box; RNA; helicase

RNA helicases are regulated by conformational changes that are driven by the binding of substrates and effector proteins. The RNA helicase Sub2 participates in many crucial processes in the nucleus, including splicing and formation of export-competent messenger ribonucleoprotein partciles (mRNPs). In particular, Sub2 functions during transcription elongation with the THO complex and in turns recruits the mRNA export factor Yra1 to form the so called transcription-export (TREX) complex. In this proposal, we aim to understand how Sub2 is activated in the context of TREX by the combined action of THO and Yra1. The information we will obtain on the export functions of Sub2 may have implications on the activation mechanisms of Sub2 in splicing, a topic studied by the group of Michael Sattler (B03).

RNA解旋酶受底物和效应蛋白结合驱动的构象变化调节。RNA解旋酶Sub 2参与细胞核中的许多重要过程，包括剪接和形成具有输出能力的信使核糖核蛋白片段（mRNP）。特别是，Sub 2在转录延伸期间与THO复合物一起发挥作用，并反过来招募mRNA输出因子Yra 1以形成所谓的转录-输出（TREX）复合物。在这个提议中，我们的目标是了解Sub 2如何在TREX的背景下通过THO和Yra 1的联合作用被激活。我们将获得的关于Sub 2的输出功能的信息可能对Sub 2在剪接中的激活机制有影响，这是Michael Sattler（B 03）小组研究的主题。