Regulation of the DEAD-box RNA helicase Sub2 by TREX (A07)

RNA解旋酶受底物和效应蛋白结合引起的构象变化调控。RNA解旋酶Sub2参与细胞核的许多关键过程，包括剪接和输出信使核糖核蛋白颗粒（mRNPs）的形成。特别是，Sub2在转录延伸过程中与THO复合物一起发挥作用，并反过来招募mRNA输出因子Yra1形成所谓的转录输出（TREX）复合物。在本提案中，我们旨在了解Sub2如何在TREX背景下通过THO和Yra1的联合作用被激活。我们将获得关于Sub2输出功能的信息，这可能对Michael Sattler （B03）小组研究的剪接中Sub2的激活机制有影响。

RNA helicases are regulated by conformational changes that are driven by the binding of substrates and effector proteins. The RNA helicase Sub2 participates in many crucial processes in the nucleus, including splicing and formation of export-competent messenger ribonucleoprotein partciles (mRNPs). In particular, Sub2 functions during transcription elongation with the THO complex and in turns recruits the mRNA export factor Yra1 to form the so called transcription-export (TREX) complex. In this proposal, we aim to understand how Sub2 is activated in the context of TREX by the combined action of THO and Yra1. The information we will obtain on the export functions of Sub2 may have implications on the activation mechanisms of Sub2 in splicing, a topic studied by the group of Michael Sattler (B03).