Collaborative Research: Fabrication and Optimization of Continuous Stationary Phase Gradients for Liquid Chromatography

合作研究：液相色谱连续固定相梯度的制备和优化

• 批准号: 1609449
• 负责人: Maryanne Collinson
• 金额: $ 44.9万
• 依托单位: Virginia Commonwealth University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1609449&HistoricalAwards=false
• 关键词: Collaborative; Research; Fabrication; Optimization; Continuous

This project is funded by the Chemical Measurement and Imaging Program of the Division of Chemistry at the National Science Foundation. The goal of a chemical separation is to separate a complex mixture into its individual components by taking advantage of differences in the degree of interaction a species exhibits between a stationary phase and a mobile phase. In this project, Professors Maryanne Collinson and Sarah Rutan at Virginia Commonwealth University (VCU) are exploring an alternate approach in the field of chemical separations. Instead of manipulating the composition of the mobile phase during the course of a separation, the stationary phase composition is instead changed. Gradual changes in stationary phases have many advantages including the ability to improve selectivity, separation times, and the ability to detect species in the mixture. The synergy between the collaborating research groups enhances the training of a diverse body of undergraduate and graduate students in state-of-the-art materials synthesis, chromatography, and the predictive power provided by simulations. In addition, by developing a peer-coaching relationship with new faculty, undergraduate instruction in analytical chemistry at VCU is being enhanced.This project focuses on the following three specific aims: (1) design, construction, and characterization of liquid chromatography columns with a continuous gradient in chemical functionality along their length, (2) development and implementation of tools to predict the chromatographic responses for various sets of analytes on a continuous gradient stationary phase, and (3) coupling simulations with experiments to determine what gradient lengths and compositions are needed to obtain optimum separations, thus avoiding the trial and error approach usually used in method development. For proof-of-principle, silica monolithic columns and columns packed with superficially porous particles are strategically modified to incorporate a continuous gradient in C8, C18, amine, and/or phenyl groups using silane chemistry and controlled rate infusion (CRI), a method recently developed in the Collinson group. The integration of the Collinson group's expertise in sol-gel chemistry and surface chemical gradients with the Rutan group's expertise in modeling of separations and liquid chromatography is leading to the development of new methodologies for problems of societal relevance, ranging from biomarker discovery to detection of low levels of environmental contaminants.

该项目由美国国家科学基金会化学部的化学测量和成像计划资助。化学分离的目的是利用物质在固定相和移动的相之间表现出的相互作用程度的差异，将复杂的混合物分离成其各个组分。在这个项目中，弗吉尼亚联邦大学（VCU）的Maryanne Collinson和Sarah Rutan教授正在探索化学分离领域的另一种方法。代替在分离过程中操纵移动的相的组成，而是改变固定相的组成。固定相的逐渐变化具有许多优点，包括提高选择性、分离时间的能力以及检测混合物中物质的能力。 合作研究小组之间的协同作用增强了对不同的本科生和研究生的培训，包括最先进的材料合成，色谱法和模拟提供的预测能力。此外，通过与新教师建立同伴指导关系，VCU的分析化学本科教学正在得到加强。该项目侧重于以下三个具体目标：（1）设计、构造和表征化学官能度沿其长度沿着连续梯度的液相色谱柱，（2）开发和实施用于预测连续梯度固定相上的各种分析物组的色谱响应的工具，以及（3）将模拟与实验相结合，以确定获得最佳分离所需的梯度长度和组成，从而避免了方法开发中通常使用的试错法。为了证明原理，二氧化硅整体柱和填充有表面多孔颗粒的柱被策略性地修改，以使用硅烷化学和控制速率注入（CRI）（Collinson组最近开发的方法）在C8、C18、胺和/或苯基中引入连续梯度。Collinson集团在溶胶-凝胶化学和表面化学梯度方面的专业知识与Rutan集团在分离和液相色谱建模方面的专业知识相结合，正在为解决社会相关问题开发新的方法，从生物标志物发现到低水平环境污染物的检测。