NSF Postdoctoral Fellowship in Biology FY 2016
2016 财年 NSF 生物学博士后奖学金
基本信息
- 批准号:1611975
- 负责人:
- 金额:$ 21.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Fellowship Award
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-07-01 至 2019-06-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This action funds an NSF National Plant Genome Initiative Postdoctoral Research Fellowship in Biology for FY 2016. The fellowship supports a research and training plan in a host laboratory for the Fellow who also presents a plan to broaden participation in biology. The title of the research and training plan for this fellowship to Bradlee Nelms is "Specification and Differentiation of Maize Archesporial Cells During Early Anther Development." The host institution for the fellowship is Stanford University and the sponsoring scientist is Dr. Virginia Walbot.The fellowship activities are focused on understanding early anther development in maize. As a reproductive organ of a major crop plant, the maize anther plays a central role in many processes of agronomic importance. Defects in early anther development lead to male sterility, decreasing grain number and yield in many cereal crops. Furthermore, one of the earliest causes of yield loss in response to heat and drought is pollen inviability; thus, understanding anther development and male fertility will be important to insuring food security in the face of climate change. This project will increase knowledge of basic reproductive biology, information that can ultimately lead to breakthroughs in crop breeding and yield enhancement. The fellowship activities will also promote teaching and learning both within academia and in the general public. The fellow will lead an undergraduate-focused outreach project to design and market educational materials, helping to communicate the broad importance of the plant sciences to the community and stimulating collaboration between undergraduates from scientific fields and other disciplines. Further educational outreach will include mentoring undergraduate and high school students in laboratory research.The overarching scientific goal of this research is to uncover early events in the development of archesporial (AR) cells--the first cell type within the anther committed to meiosis and pollen production. Major aims are to (1) identify cellular intermediates as AR cells differentiate into meiocytes and (2) investigate the role of TGA-type transcription factors in AR cell specification. These aims will be pursued using a combination of genomics, genetics, and biochemistry. Single-cell RNA sequencing will allow the identification of distinct AR cell populations, which will then be ordered into a developmental pathway by integrating transcriptome data with in situ localization of RNA transcripts. The subcellular localization, redox status, and DNA binding activity of several TGA transcription factors will be assessed in early stage anthers under various genetic and physiological perturbations, and downstream signaling pathways for the TGA factors will be evaluated by chromatin immunoprecipitation. Together, the fellowship activities will generate substantial transcriptomic and ChIP-Seq data, clarify the differentiation program of AR cell development, and help to characterize the regulation and signaling of TGA family transcription factors during AR cell specification in a spatio-temporal context. Research results will be presented at national conferences and published in peer-reviewed journals, and all sequence data will be submitted to public repositories such as the NCBI Sequence Read Archive (http://www.ncbi.nlm.nih.gov/sra) and Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/).
本行动资助2016财年美国国家科学基金会国家植物基因组计划生物学博士后研究奖学金。该奖学金支持奖学金获得者在主办实验室的研究和培训计划,该奖学金获得者还提出了扩大生物学参与的计划。布拉德利·尼尔姆斯的研究和培训计划的标题是“早期花药发育过程中玉米孢原细胞的规格和分化”。该奖学金的主办机构是斯坦福大学,赞助科学家是弗吉尼亚·沃尔伯特博士。研究活动的重点是了解玉米的早期花药发育。玉米花药作为一种重要作物的生殖器官,在许多重要的农艺过程中起着核心作用。在许多谷类作物中,早期花药发育缺陷导致雄性不育,降低粒数和产量。此外,高温和干旱导致产量损失的最早原因之一是花粉无活力;因此,了解花药发育和雄性生殖能力对于在气候变化的情况下确保粮食安全至关重要。该项目将增加基本生殖生物学的知识,这些信息最终可以在作物育种和产量提高方面取得突破。奖学金活动还将促进学术界和一般公众的教学。该研究员将领导一个以本科生为重点的外展项目,设计和销售教育材料,帮助向社区传达植物科学的广泛重要性,并促进来自科学领域和其他学科的本科生之间的合作。进一步的教育拓展将包括指导本科生和高中生进行实验室研究。本研究的首要科学目标是揭示原始孢子(AR)细胞发育的早期事件,这是花药中致力于减数分裂和花粉产生的第一种细胞类型。主要目的是:(1)鉴定AR细胞分化为减数细胞时的细胞中间体;(2)研究tga型转录因子在AR细胞分化中的作用。这些目标将通过基因组学、遗传学和生物化学的结合来实现。单细胞RNA测序将允许识别不同的AR细胞群,然后通过整合转录组数据和RNA转录物的原位定位,将其排序到发育途径中。TGA转录因子的亚细胞定位、氧化还原状态和DNA结合活性将在各种遗传和生理扰动下的早期花药中进行评估,TGA转录因子的下游信号通路将通过染色质免疫沉淀进行评估。总之,该合作活动将产生大量转录组学和ChIP-Seq数据,阐明AR细胞发育的分化程序,并有助于在时空背景下表征AR细胞规范过程中TGA家族转录因子的调控和信号传导。研究成果将在全国会议上发表,并在同行评议的期刊上发表,所有序列数据将提交给NCBI序列读取档案(http://www.ncbi.nlm.nih.gov/sra)和基因表达Omnibus (http://www.ncbi.nlm.nih.gov/geo/)等公共存储库。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Bradlee Nelms其他文献
Bradlee Nelms的其他文献
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{{ truncateString('Bradlee Nelms', 18)}}的其他基金
TRTech-PGR: Reprogramming the Plant Cell with Transcription Factors
TRTech-PGR:用转录因子对植物细胞进行重编程
- 批准号:
2315723 - 财政年份:2023
- 资助金额:
$ 21.6万 - 项目类别:
Standard Grant
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