Surface- and contact-dependent motility in the soil bacterium Myxococcus xanthus (E08 (C04))

黄色粘球菌的细胞经历了主要的生活方式的变化，以响应营养可获得性，形成了两个形态上不同的生物膜。IV型菌毛(T4P)依赖的运动性对于这两种生物膜的形成是必不可少的。我们提出了两条研究路线来理解T4P的机制。首先，在我们T4P机制模型的基础上，我们的目标是确定T4P机制底部的蛋白质如何动态相互作用。其次，我们的目标是阐明黄曲霉胞外多糖(EPS)的组成和结构，并确定c-di-GMP如何调节黄曲霉胞外多糖的积累，从而确定T4P在黄曲霉中的作用。

Cells of Myxococcus xanthus undergo major lifestyle changes in response to nutrient availability with the formation of two morphologically distinct biofilms. Type IV pili (T4P)-dependent motility is essential for the formation of both types of biofilms. We propose two lines of investigations to understand the mechanism of T4P. First, building on our model for the T4P machinery, we aim to determine how proteins at the base of the T4P machinery interact dynamically. Second, we aim to elucidate the composition and structure of M. xanthus exopolysaccharides (EPS) and determine how c-di-GMP regulates EPS accumulation and in that way T4P function in M. xanthus.