磷脂分子参与植物细胞器互作及自噬的调控机制

磷脂酰肌醇（PI）信号途径在植物生长发育以及细胞对环境信号、激素反应的信号传递过程中发挥重要调节作用。课题组的前期研究表明磷脂酰肌醇信号和磷脂分子参与了植物激素、光信号的传递，并阐明了相关调控机制，有较好的研究积累。拟在此基础上，建立基于植物细胞器的脂质组学分析平台，综合利用遗传学、生物化学、细胞生物学等手段，系统研究磷脂转运蛋白通过转运磷脂分子参与细胞器结构和功能维持、介导细胞器互作的作用及其机理，重点阐明磷脂转运蛋白通过转运磷脂介导内质网和叶绿体互作的作用及机制、磷脂酸转运蛋白通过转运磷脂酸介导线粒体和细胞核互作的作用及机制；肌醇多磷酸磷酸酶及其产物肌醇磷酸分子/钙离子、以及磷脂酶及其产物磷脂酸调控细胞自噬及介导自噬体与其它细胞器融合的机制；进一步揭示植物细胞器互作在细胞信号传递、植物生长发育中的调控作用及机制。

Phosphatidylinositol (PI) signaling pathway plays crucial roles in plant growth and development and cell responses to environmental signals, such as light, temperature and plant hormones. Our previous studies revealed that PI signaling pathway was involved in the effects and signaling transduction of hormones, sucrose and light, and disclosed the regulatory mechanisms. Based on the previous basis, we will study the mechanism that phospholipids mediate the structural and functional maintenance of organelles, and the interaction of organelles. We will mainly focus on: 1) the functional interaction between endoplasmic reticulum (ER) and chloroplast mediated by phosphatidylinositol transfer protein (PITP) and phospholipids; 2) the functional interaction between mitochondrion and nucleus mediated by phosphatidic acid (PA) transfer protein and PA; 3) the functional mechanism of autophage and mediated the contact with other organelles by inositol polyphosphate 5-phosphatase, phospholipase and their products (PA, IP3, Ca2+). We then further revealed that the regulatory mechanism of organelle interaction functions on cellular signaling transduction and plant growth and development.