EAPSI: Identifying Fungi Associated with the Ancient Conifer Agathis Australis and Testing their Potential as Biological Control Agents against Harmful Pathogen

EAPSI：鉴定与古代针叶树 Agathis Australis 相关的真菌并测试其作为有害病原体生物防治剂的潜力

• 批准号: 1613884
• 负责人: Lauren Jackson
• 金额: $ 0.04万
• 依托单位: Jackson Lauren W
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1613884&HistoricalAwards=false
• 关键词: EAPSI; Identifying; Fungi; Associated; Ancient

Kauri (Agathis australis) are economically and culturally important trees that are native to New Zealand and are currently threatened by a devastating soil pathogen that causes a disease known as kauri dieback. In collaboration with Dr. Maj Padamsee of Landcare Research in Auckland, New Zealand, who has expertise working with kauri dieback and fungi that associate with kauri, this work aims to (1) identify beneficial fungi that associate with the roots of kauri; and (2) evaluate their potential to inhibit infection or slow the progression of the pathogen. The network formed to complete this work will be beneficial for professional development and future international collaborations. Findings from this research have the potential to improve control methods for kauri dieback and diseases caused by similar pathogens such as sudden oak death, soybean root rot and cocoa black pod disease that threaten economically and culturally important trees in other regions around the globe.Agathis australis (Araucariaceae), commonly referred to as kauri, is an ancient, long-lived, economically important and culturally significant conifer that is endemic to New Zealand. Kauri have been reduced from 1.6 million hectares to only ca. 7500 hectares since European arrival and the remaining trees are currently threatened by Phytophthora agathicida, an oomycete pathogen that causes a devastating collar rot known as kauri dieback. Padamsee and colleagues characterized mycorrhizal associations with kauri roots and found evidence of dark septate endophytes (DSEs) also being present in association with the roots via microscopic analyses. The scope of the present work is to (1) identify kauri-associated DSE fungi at the species level by sequencing their internal transcribed spacer (ITS) regions; and (2) evaluate the potential of DSE fungi to inhibit vegetative growth and spore germination rates of P. agathicida via two in vitro inhibition assays. Results from this investigation may serve as the basis for biocontrol methods that can be applied to kauri dieback and other important diseases that are caused by Phytophthora spp.This award under the East Asia and Pacific Summer Institutes program supports summer research by a U.S. graduate student and is jointly funded by NSF and the Royal Society of New Zealand.

贝壳杉（Agathis australis）是新西兰本土的经济和文化重要树木，目前受到一种破坏性土壤病原体的威胁，这种病原体会导致一种称为贝壳杉枯死的疾病。与新西兰奥克兰土地护理研究所的Maj Padamsee博士合作，他具有与贝壳杉枯死和与贝壳杉相关的真菌相关的专业知识，这项工作旨在（1）确定与贝壳杉根部相关的有益真菌;（2）评估它们抑制感染或减缓病原体进展的潜力。为完成这项工作而建立的网络将有利于专业发展和未来的国际合作。这项研究的结果有可能改进贝壳杉枯死和由类似病原体引起的疾病的控制方法，如橡树猝死症、大豆根腐病和可可黑荚病，这些疾病威胁着地球仪其他地区的经济和文化重要树木。（南洋杉科），通常被称为贝壳杉，是一种古老的、长寿的、经济上重要的和文化上重要的针叶树，是新西兰特有的。贝壳杉已从160万公顷减少到只有约。自欧洲人到来以来，已有7500公顷的树木受到了Phytophthora agathicida的威胁，Phytophthora agathicida是一种卵菌病原体，会导致毁灭性的衣领腐烂，称为贝壳杉枯死。Padamsee及其同事描述了与贝壳杉根的菌根关联，并通过显微镜分析发现了暗隔膜内生菌（DSE）也与根相关的证据。本工作的范围是（1）通过对贝壳杉内转录间隔区（ITS）区域进行测序，在物种水平上鉴定贝壳杉相关DSE真菌;（2）通过两种体外抑制试验，评估DSE真菌抑制P. agathicida营养生长和孢子萌发率的潜力。从这次调查的结果可以作为生物防治方法的基础，可应用于贝壳杉顶枯病和其他重要的疾病，是由疫霉属。东亚和太平洋夏季研究所计划下的这一奖项支持美国研究生的夏季研究，是由美国国家科学基金会和新西兰皇家学会共同资助。