Control of Na+ and H+ transport in bacterial adaptation

细菌适应中Na和H运输的控制

• 批准号: 1616674
• 负责人: Blanca Barquera
• 金额: $ 60万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1616674&HistoricalAwards=false
• 关键词: Control; Na+; H+; transport; bacterial

The overall goal of this project is to understand the mechanism by which bacteria survive and proliferate in many different environments. Transport proteins in the membrane of bacteria are largely responsible for these adaptations. The aim of this project is to understand how these proteins allow bacteria to adjust their metabolism to different environmental conditions. This knowledge will contribute to the field of bacterial ecology. This knowledge will also contribute to many areas of biotechnology. For example, engineering bacteria that are capable of specific adaptive responses is important for waste remediation, metabolic engineering, and bacterial production of natural and artificial products. This project will expose high school students and undergraduate students to research. Efforts to broaden participation in the sciences are a major component of this project. The overall objective of the project is to understand bacterial adaptation at the molecular level. The goal is to understand the mechanism by which Pseudomonas aeruginosa adapts to different environments through the operation of its proton and sodium transporters, and through changes in expression patterns of the various branches of its respiratory chain and its sodium/proton antiporters. There are two goals in the project: 1) defining which of these enzymes are expressed as Pseudomonas aeruginosa adapts to a range of culture conditions and growth phases; 2) defining the roles of the component enzymes by constructing strains in which one or more of these enzymes have been deleted. Sodium/proton antiporter deletion strains will be used as backgrounds for studying the role of the NADH dehydrogenases and their deletions, by disconnecting the sodium and proton gradients. The role of the three NADH dehydrogenases and the antiporters, and how changes in their expression support adaptation, will be defined. The project will contribute to the basic understanding of how bacteria adapt to different environments. This knowledge is essential for almost any application involving bacteria including biological waste remediation and the use of bacteria to synthesize natural and artificial products. It will also contribute to development of new ways to fight bacterial diseases including those caused by antibiotic resistant strains.

该项目的总体目标是了解细菌在许多不同环境中生存和增殖的机制。细菌膜中的转运蛋白在很大程度上负责这些适应。该项目的目的是了解这些蛋白质如何允许细菌调整其代谢以适应不同的环境条件。这些知识将有助于细菌生态学领域。 这些知识也将有助于生物技术的许多领域。 例如，能够进行特定适应性反应的工程细菌对于废物修复、代谢工程以及天然和人工产品的细菌生产是重要的。 这个项目将使高中生和本科生接触到研究。 努力扩大对科学的参与是该项目的一个主要组成部分。 该项目的总体目标是在分子水平上了解细菌的适应性。目的是了解铜绿假单胞菌通过其质子和钠转运蛋白的运作，以及通过其呼吸链及其钠/质子反向转运蛋白的各个分支的表达模式的变化来适应不同环境的机制。该项目有两个目标：1）定义铜绿假单胞菌适应一系列培养条件和生长阶段时表达哪些酶; 2）通过构建其中一种或多种酶的菌株来定义组分酶的作用已被删除。通过断开钠和质子梯度，钠/质子反向转运蛋白缺失菌株将用作研究NADH脱氢酶及其缺失的作用的背景。三个NADH脱氢酶和反向转运蛋白的作用，以及如何在他们的表达支持适应的变化，将被定义。该项目将有助于对细菌如何适应不同环境的基本了解。这一知识对于几乎所有涉及细菌的应用都是必不可少的，包括生物废物修复和使用细菌合成天然和人工产品。它还将有助于开发对抗细菌疾病的新方法，包括由抗生素耐药性菌株引起的细菌疾病。