DNA-storing and ejection from Chlorella viruses

小球藻病毒在其基因组中编码K+通道。数据支持该通道存在于病毒体中的假设。在感染过程中，它被插入小球藻宿主细胞的质膜，并引起去极化（<$Em）。选择性阻断该通道可抑制病毒诱导的Em，并在DNA排出步骤中阻止病毒复制。本项目的目的是了解产生的病毒通道的生理重要性。实验旨在研究其在降低宿主的膨压方面的意义以及由此对病毒DNA排出的后果。进一步的实验将解决这个问题，即Em是否对宿主细胞质的离子环境有影响。这可能在致病病毒关闭宿主细胞代谢的策略中发挥作用。进一步的实验计划检查的重要性，在触发过程中，防止聚感染。这种机制允许单个病毒防止第二种病毒感染细胞。该项目将共同提供有关病毒如何进入并破坏其主机的一般问题的基本信息。

Chlorella viruses code in their genome for a K+ channel. Data support the hypothesis that this channel is present in the virion. During infection it is inserted into the plasma membrane of the Chlorella host cell and causes a depolarization (¿Em). Selective block of the channel inhibits the virus-induced ¿Em and prevents viral replication at the step of DNA ejection. Aim of the present project is to understand the physiological importance of the viral channel generated ¿Em. Experiments are designed to examine its significance in lowering the turgor pressure of the host and the consequence of this on DNA ejection from the virus. Further experiments will address the question whether the ¿Em has a consequence on the ion milieu of the host cell cytoplasm. This could play a role in the strategy of the pathogenic viruses in shutting off host cell metabolism. Further experiments are planned to examine the importance of ¿Em in triggering a process, which prevents poly infection. This mechanism allows an individual virus to prevent infection of a cell by a second virus. Together this project will provide basic information on the general question on how viruses enter and corrupt their host.