DNA-storing and ejection from Chlorella viruses

小球藻病毒的 DNA 储存和喷射

• 批准号: 22483695
• 负责人: Professor Dr. Gerhard Thiel
• 金额: --
• 依托单位: Arbeitsgruppe Plant Membrane Biophysics
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/22483695?language=en
• 关键词: DNA; storing; ejection; Chlorella; viruses

Chlorella viruses code in their genome for a K+ channel. Data support the hypothesis that this channel is present in the virion. During infection it is inserted into the plasma membrane of the Chlorella host cell and causes a depolarization (¿Em). Selective block of the channel inhibits the virus-induced ¿Em and prevents viral replication at the step of DNA ejection. Aim of the present project is to understand the physiological importance of the viral channel generated ¿Em. Experiments are designed to examine its significance in lowering the turgor pressure of the host and the consequence of this on DNA ejection from the virus. Further experiments will address the question whether the ¿Em has a consequence on the ion milieu of the host cell cytoplasm. This could play a role in the strategy of the pathogenic viruses in shutting off host cell metabolism. Further experiments are planned to examine the importance of ¿Em in triggering a process, which prevents poly infection. This mechanism allows an individual virus to prevent infection of a cell by a second virus. Together this project will provide basic information on the general question on how viruses enter and corrupt their host.

小球藻病毒在其基因组中编码一个K+通道。数据支持该通道存在于病毒粒子中的假设。在感染期间，它被插入小球藻宿主细胞的质膜并引起去极化（¿Em）。选择性阻断通道抑制病毒诱导的¿Em，并在DNA喷射的步骤中阻止病毒复制。本项目的目的是了解产生的病毒通道的生理重要性。实验旨在检验其在降低宿主膨胀压力方面的意义，以及这对病毒DNA喷射的影响。进一步的实验将解决¿Em是否对宿主细胞质的离子环境有影响的问题。这可能在致病性病毒阻断宿主细胞代谢的策略中起作用。进一步的实验计划检验¿Em在触发一个防止多聚感染的过程中的重要性。这种机制允许单个病毒防止另一种病毒感染细胞。总之，这个项目将提供关于病毒如何进入和破坏宿主的一般问题的基本信息。