DNA-storing and ejection from Chlorella viruses

小球藻病毒在它们的基因组中编码K通道。数据支持病毒粒子中存在这一通道的假设。在感染期间，它被插入到小球藻宿主细胞的质膜中，引起去极化(？Em)。选择性阻断该通道可抑制病毒诱导的Em，并在DNA排出阶段阻止病毒复制。本项目的目的是了解病毒通道产生的Em的生理重要性。实验的目的是检查它在降低宿主膨胀压力方面的意义以及这对DNA从病毒中排出的后果。进一步的实验将解决Em是否对宿主细胞细胞质的离子环境产生影响的问题。这可能在致病病毒切断宿主细胞代谢的策略中发挥作用。计划进行进一步的实验，以检验Em在触发防止多发性感染的过程中的重要性。这一机制允许单个病毒防止另一种病毒感染细胞。该项目将共同提供有关病毒如何进入和破坏其宿主的一般问题的基本信息。

Chlorella viruses code in their genome for a K+ channel. Data support the hypothesis that this channel is present in the virion. During infection it is inserted into the plasma membrane of the Chlorella host cell and causes a depolarization (¿Em). Selective block of the channel inhibits the virus-induced ¿Em and prevents viral replication at the step of DNA ejection. Aim of the present project is to understand the physiological importance of the viral channel generated ¿Em. Experiments are designed to examine its significance in lowering the turgor pressure of the host and the consequence of this on DNA ejection from the virus. Further experiments will address the question whether the ¿Em has a consequence on the ion milieu of the host cell cytoplasm. This could play a role in the strategy of the pathogenic viruses in shutting off host cell metabolism. Further experiments are planned to examine the importance of ¿Em in triggering a process, which prevents poly infection. This mechanism allows an individual virus to prevent infection of a cell by a second virus. Together this project will provide basic information on the general question on how viruses enter and corrupt their host.