MRI: Acquisition of a matrix-assisted laser desorption/ionization, time-of-flight (MALDI TOF) mass spectrometer at Xavier University of Louisiana

有了这个奖项从主要研究仪器计划（MRI）和支持化学研究仪器（CRIF）和历史上黑人学院和大学本科生计划（HBCU-UP）以及实验计划，以刺激竞争性研究（EPSCoR），来自路易斯安那州泽维尔大学的Terry Watt教授及其合作者获得了一台基质辅助激光解吸/电离飞行时间质谱仪（MALDI-TOF质谱仪）。一般来说，质谱（MS）是用于识别和表征嵌入复杂基质中的少量化学物质的关键分析方法之一。在MALDI仪器中，激光撞击嵌入样品的非反应性支撑材料，使样品蒸发并使其形成带电粒子（离子）。离子进入质谱仪，在那里测量母离子及其碎片离子的质量。在飞行时间仪器中，离子被电场加速以允许进一步表征。MALDI TOF结合了温和的离子化（非常适合产生肽、蛋白质、核酸、碳水化合物、合成聚合物和其他类似大小的物质的完整离子）和检测模式，在广泛的质量范围内提供灵敏度和准确度之间的出色平衡。碰撞诱导解离使分子离子在气相中分裂成碎片。这种高灵敏度的技术可以识别和确定复杂混合物中分子的结构。此次收购加强了大学和区域的研究基础设施。该仪器扩大了参与，让不同的学生参与研究和研究培训，这种现代分析技术。 该仪器与杜兰大学的学生共享。该奖项旨在加强该HBCU各级的研究和教育，特别是在以下领域：（a）表征赖氨酸脱乙酰酶的酶-底物相互作用;（B）开发与葡萄糖氧化酶偶联的小分子检测适体，作为生物传感器;（c）使用修饰的核碱基开发水凝胶;（d）开发生物传感器。（d）使用具有精确限定的配体含量的修饰的核碱基水凝胶和树枝状聚合物用于靶向和受控的药物释放;以及（e）鉴定转录因子结合位点和结合配偶体。

With this award from the Major Research Instrumentation Program (MRI) and support from the Chemistry Research Instrumentation (CRIF) and the Historically Black Colleges and Universities-Undergraduate Program (HBCU-UP) as well as the Experimental Program to Stimulate Competitive Research (EPSCoR), Professor Terry Watt from Xavier University of Louisiana and collaborators have acquired a matrix assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF mass spectrometer). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a MALDI instrument, a laser striking on the unreactive supporting material embedded with the sample, vaporizes the sample and causes it to form charged particles (ions). The ions pass into the mass spectrometer where the masses of the parent ion and its fragment ions are measured. In a time-of-flight instrument the ions are accelerated by an electric field to allow further characterization. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide mass range. The collision-induced dissociation enables the molecular ions to break into pieces in the gas phase. This highly sensitive technique allows identification and determination of the structure of molecules in a complex mixture. This acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse students in research and research training with this modern analytical technique. The instrument is shared with students at Tulane University. The award is aimed at enhancing research and education at all levels at this HBCU, especially in areas such as (a) characterizing enzyme-substrate interactions of lysine deacetylases; (b) developing aptamers for small molecule detection coupled to glucose oxidase as a biosensor; (c) developing hydrogels using modified nucleobases; (d) using modified nucleobase hydrogels and dendrimers with precisely defined ligand content for targeted and controlled drug release; and (e) identifying transcription factor binding sites and binding partners.