MRI: Acquisition of a Matrix-Assisted Laser Desorption/Ionization Time-of-Flight(MALDI TOF) Mass Spectrometer for of Research and Research Training at CSULA

该奖项由重大研究仪器项目资助。加州州立大学洛杉矶分校的巴勇教授及其同事Jamil Momand、Edith Porter、Wang Ixian Wang和Cecilia Zurita-Lopez正在获得一台配备光电二极管阵列的基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF-MS)。通常，质谱仪是用于鉴定和表征复杂样品中少量化学物种的关键分析方法之一。MALDI TOF结合了温和的电离(非常适合生产完整的多肽、蛋白质、核酸、碳水化合物、合成聚合物和其他类似大小物种的离子)和检测模式，在各种样品的灵敏度和准确性之间提供了极好的平衡。此次收购加强了该大学和该地区的研究基础设施。该工具通过向不同的学生群体提供亲身实践机会来扩大参与度。该仪器还用于外展活动。该奖项旨在加强各级的研究和教育。它尤其影响了与分子结构、取向和动力学相关的研究。研究人员使用MALDI-TOF-MS研究粘附性共聚胶体凝胶体系的相互作用，并在哺乳动物细胞中确定精氨酸甲基转移酶7(PRMT7)蛋白的体内底物和结合伙伴。质谱仪用于研究可能在帕金森氏病中起作用的细胞破坏机制，并用于研究抗冻蛋白。该仪器还为研究人员识别与t-DARPP AKT激活蛋白相关的蛋白质提供服务，其中t-DARPP是多巴胺调节的磷蛋白的截短异构体，具有与钙结合的拉长单体，并被细胞周期蛋白依赖的酶激酶磷酸化。最后，该仪器被研究抗菌肽的研究人员使用。总体而言，收购仪器可能会使国家健康和福利以及支持教育和多样性受益。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation Program. Professor Yong Ba from California State University Los Angeles and colleagues Jamil Momand, Edith Porter, Yixian Wang and Cecilia Zurita-Lopez are acquiring a matrix-assisted laser desorption / ionization time-of-flight mass spectrometer (MALDI-TOF-MS) equipped with a photodiode array. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide range of samples. The acquisition strengthens the research infrastructure at the university and within the regional. The instrument broadens participation by giving hands-on access to a diverse student population. The instrument is also used in outreach activities.The award is aimed at enhancing research and education at all levels. It especially impacts studies correlating molecular structure, orientation and dynamics. Researchers use the MALDI-TOF-MS to study interactions of a mucoadhesive co-polymer colloidal gel systems and in identifying in vivo substrates and binding partners for the protein arginine methyltransferase 7 (PRMT7) in mammalian cells. The mass spectrometer is used for studying cell disruption mechanisms that may be operational in Parkinson's disease and for studying antifreeze proteins. The instrument also serves researchers identifying proteins associated with t-Darpp AKT activation proteins, in which t-Darpp is a truncated isoform of dopamine-regulated phosphoprotein having an elongated monomer that binds calcium and is phosphorylated by cyclin-dependent enzyme kinases. Finally, the instrument is used by researchers investigating antimicrobial peptides. Overall, the instrument acquisition may benefit the National health and welfare as well as supporting education and diversity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.