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Barrier properties of biological hydrogel filters

生物水凝胶过滤器的阻隔性能

基本信息

批准号：
225109143
负责人：
Professor Dr. Oliver Lieleg
金额：
--
依托单位：
Munich School of Engineering
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/225109143?language=en
关键词：
Barrier properties biological hydrogel filters

项目摘要

The aim of this project is to characterize the barrier properties of two important biological hydrogels in vitro: vitreous humor and neurofilament gels. A key aspect of this study is to elucidate the contribution of electrostatic interactions to the regulation of particle diffusion inside the hydrogels. We will use test particles with well-defined properties such as particle size and surface charge and will quantify their diffusion behavior in the hydrogel environments. The insights obtained from the experiments with vitreous humor and neurofilament gels will be compared with previous results on particle diffusion in mucus and extracellular matrix to help us identify the biochemical key motifs that establish selective filtering in biological hydrogels.So far, the barrier function of biological hydrogels has mainly been studied by medical researchers in vivo using animal models or complex ex vivo tissues. However, in those complex systems it is highly difficult to relate the observed effects to their microscopic origins. Here, we will overcome this limitation by studying (partially) purified and reconstituted hydrogel systems which will give us a much broader range of control over the experimental conditions than it is possible with native samples. The anticipated results from this project will not only provide us with a better understanding of the physical principles that regulate diffusion in biological hydrogels, but will also pinpoint design strategies for the development of new drug delivery agents or hydrogels with biomedical applications such as implant coatings or artificial tissues. Therefore, this project will establish an important link between polymer physics, cell biology, medical engineering and drug delivery.

本项目的目的是表征两种重要的生物水凝胶在体外的屏障性能：玻璃体液和神经丝凝胶。本研究的一个关键方面是阐明静电相互作用的贡献，调节水凝胶内的颗粒扩散。我们将使用具有明确属性（如粒度和表面电荷）的测试颗粒，并将量化其在水凝胶环境中的扩散行为。将玻璃体液和神经丝凝胶的实验结果与之前的粘液和细胞外基质中颗粒扩散的结果进行比较，以帮助我们确定生物水凝胶中建立选择性过滤的生化关键基序。迄今为止，生物水凝胶的屏障功能主要由医学研究人员使用动物模型或复杂的离体组织在体内进行研究。然而，在这些复杂的系统中，很难将观察到的效应与其微观起源联系起来。在这里，我们将通过研究（部分）纯化和重构的水凝胶系统来克服这种限制，这将为我们提供比天然样品更广泛的实验条件控制范围。该项目的预期结果不仅将使我们更好地理解调节生物水凝胶中扩散的物理原理，而且还将为开发具有生物医学应用（如植入涂层或人工组织）的新药物递送剂或水凝胶确定设计策略。因此，该项目将在高分子物理学、细胞生物学、医学工程和药物输送之间建立重要联系。