A microfluidics approach to study the selectivepermeability of native and chemically modifiedmucin gels

研究天然和化学修饰粘蛋白凝胶选择性渗透性的微流体方法

• 批准号: 397368393
• 负责人: Professor Dr. Oliver Lieleg
• 金额: --
• 依托单位: Munich School of Engineering
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/397368393?language=en
• 关键词: microfluidics; approach; study; selectivepermeability; native

In this project we aim at deciphering the molecular mechanisms responsible for the selective barrier properties of mucin gels. This will be achieved by combining molecular penetration experiments with a damage-and-repair approach, i.e. performing chemical modifications on purified mucin glycoproteins. This strategy will allow us to identify molecular key motifs on mucin glycoproteins which are necessary to establish selective permeability in mucin gels. The anticipated results will give insights into the physico-chemical principles that provide mucus with its protective function and may guide the path towards the development of synthetic mucin-like molecules.

在这个项目中，我们的目标是破译负责粘蛋白凝胶的选择性屏障性能的分子机制。这将通过将分子渗透实验与损伤修复方法相结合来实现，即对纯化的粘蛋白糖蛋白进行化学修饰。这种策略将使我们能够确定粘蛋白糖蛋白的分子关键基序，这是必要的，以建立粘蛋白凝胶的选择性渗透性。预期的结果将深入了解为粘液提供保护功能的物理化学原理，并可能指导合成粘蛋白样分子的发展。

项目成果

Advances in Mucin Biopolymer Research: Purification, Characterization, and Applications

• DOI: 10.1002/9783527818310.ch6
• 发表时间: 2021-02
• 作者: M. Marczynski;Benjamin Winkeljann;O. Lieleg

Purified mucins in drug delivery research.

• DOI: 10.1016/j.addr.2021.113845
• 发表时间: 2021-06
• 期刊: Advanced drug delivery reviews
• 影响因子: 16.1
• 作者: M. Marczynski;Ceren Kımna;O. Lieleg