RNA elements and protein factors required for pre-ribosome remodelling during 60S biogenesis in yeast (A12)

酵母 60S 生物发生过程中前核糖体重塑所需的 RNA 元件和蛋白质因子 (A12)

• 批准号: 226425436
• 金额: --
• 依托单位: Institut für Molekularbiologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/226425436?language=en
• 关键词: RNA; elements; protein; factors; required

Ribosome biogenesis in eukaryotes is a major cellular pathway that requires multiple cofactors, among them DEAD/H-box RNA helicases. We have identified binding sites of multiple large subunit RNA helicases on yeast ribosomal RNAs and analyse their molecular functions in the pathway. Our data also reveal interactions between several helicases and non-ribosomal RNAs, suggesting that these proteins perform additional functions in other pathways. Understanding the molecular functions and regulation of RNA helicases as well as their interplay will shed light on key steps in RNA metabolism and the cross-regulation of different cellular processes.

真核生物的核糖体生物发生是一个主要的细胞途径，需要多种辅助因子，其中包括DEAD/H-box RNA解旋酶。我们已经确定了酵母核糖体RNA上多个大亚基RNA解旋酶的结合位点，并分析了它们在该途径中的分子功能。我们的数据还揭示了几种解旋酶和非核糖体rna之间的相互作用，表明这些蛋白质在其他途径中发挥额外的功能。了解RNA解旋酶的分子功能和调控及其相互作用将有助于揭示RNA代谢的关键步骤和不同细胞过程的交叉调控。