EAGER: Collaborative Research: Some Effects of Weak Electric and Magnetic Fields on Biological Systems

PI：巴恩斯/比恩提案编号：1644371/1644384关于电场和磁场可能对健康产生的影响的争议已经在雷达脉冲、电力线、手机和其他电磁场源（如Wi-Fi、电视和计算机）方面提出。许多孤立的实验表明，弱场可以改变生物系统的功能，但没有一个总体机制（S）连接场和效应是已知的。 这项工作的目的是调查的基本联系-从物理学，通过化学生物学-场和它们似乎影响的对象之间。 这项工作将建立在理论和实验工作的基础上，这些工作表明弱电场和磁场可以改变自由基对的重组时间和自由基的浓度，如活性氧（ROS），并可以改变正常和癌细胞和Planarians的生长速度。 通过提供改进的能力来指定将可重复地抑制或加速细胞（例如癌症）生长的暴露条件，将实现广泛的影响，使得这些场可以在实验和治疗上使用。 该项目由一个新成立的合作组织领导，将在两所大学对研究生和本科生进行交叉培训：科罗拉多大学电气工程专业的学生将接触生物、化学和物理，而西密歇根大学的生物医学学生将承担工程和物理实践经验。关于电磁场的生物效应的智力挑战一直是建立一个因果逻辑链，从物理学到化学，再到生物学，再到潜在的健康影响，潜在的临床用途，以及探索其他生物学问题的潜在工具。许多孤立的实验表明，弱场可以改变生物系统的功能。虽然没有已知的连接场和效应的总体机制，但在某些情况下涉及自由基。这项工作旨在提供一系列测量，更完整地表征弱磁场对细胞生长的影响。 将进行实验和理论工作，以检查从小于1 μ T到1 mT的这些场对a）细胞生长速率，B）自由基浓度和c）纤维肉瘤HT 1080细胞，成纤维细胞和Planarian扁形虫在标准细胞培养箱内的磁屏蔽插入物中的膜电位的影响。静态磁场数据将与自由基浓度变化的理论预测进行比较。将根据振幅、频率、重复率和暴露时间长度进行时变磁场测量。在低频（小于2 kHz）下进行的测量将通过使用环形盘以及在单独的电场暴露系统中将直接磁场效应与感应电场和感应电流的效应分开。从1到10兆赫的测量将寻找超精细过渡的影响，预测在地球上的氢原子？作为振幅、频率、暴露时间和静磁场的函数的45 μ T附近的磁场。这项工作将侧重于核自旋与自由基对片段内的活性电子之间的耦合，以及生物周期时间，范围从几秒钟到几小时或更长。将使用O-2（超氧化物）、NADPH（烟酰胺腺嘌呤二核苷酸磷酸氧化酶）和H2 O2（过氧化氢）的荧光染料进行自由基浓度测量，并检查膜电位的变化。 由于拟议的工作而获得的新信息应该有助于我们了解雷达脉冲，电力线，手机，Wi-Fi，电视，计算机等领域的暴露条件，可能会也可能不会对健康造成影响。 通过提供改进的能力来指定将可重复地抑制或加速细胞（例如癌症）生长的暴露条件，将实现广泛的影响，使得这些场可以在实验和治疗上使用。该项目将在两所大学对研究生和本科生进行交叉培训：科罗拉多大学的电气工程专业学生将接触生物、化学和物理，而西密歇根大学的生物医学专业学生将获得工程和物理实践经验。

PIs: Barnes/BeaneProposal Numbers: 1644371/1644384Controversy over possible health effects of electric and magnetic fields has been raised with respect to radar pulses, power lines, cell phones and other sources of electromagnetic fields such as Wi-Fi, TV and computers. Many isolated experiments have shown that weak fields can modify the function of biological systems but no overarching mechanism(s) linking fields and effects are known. The proposed work aims to investigate the fundamental link--from the physics though the chemistry to the biology--between the fields and the objects they seem to influence. The work will build on theoretical and experimental work that shows weak electric and magnetic fields can modify the recombination times for radical pairs and concentrations of radicals such as reactive oxygen species (ROS) and can modify the growth rate of normal and cancer cells and planarians. Broad impact will be achieved by providing an improved ability to specify exposure conditions that will inhibit or accelerate the growth of cells such as cancers reproducibly so that these fields can be used both experimentally and therapeutically. This project, led by a newly formed collaboration, will result in the cross-training of graduate and undergraduate students at two universities: electrical engineering students at the University of Colorado will gain exposure to biology, chemistry and physics, while biomedical students at Western Michigan University will undertake engineering and physics practical experiences.The intellectual challenge concerning the biological effects of electromagnetic fields has been to build a cause and effect chain of logic from the physics through the chemistry to the biology and on to potential health effects, potential clinical uses, and potential tools to probe other biological questions. Many isolated experiments have shown that weak fields can modify the function of biological systems. Though no overarching mechanism(s) linking fields and effects are known; radicals have been implicated in some instances. This work seeks to provide a series of measurements that more completely characterizes the effects of weak magnetic fields on cell growth. Experimental and theoretical work will be carried out to examine the effects of these fields from less than 1 ìT to 1 mT on a) cell growth rates, b) radical concentrations, and c) membrane potentials in fibrosarcoma HT1080 cells, fibroblast cells and planarian flatworms in a magnetically shielded insert inside standard cell culture incubators. Static magnetic field data will be compared with the theoretical predictions for changes in radical concentrations. Time varying magnetic field measurements will be made as functions of amplitude, frequency, repetition rate and length of exposure. Measurements made at low frequencies (less than 2 kHz) will separate direct magnetic field effects from the effects of induced electric fields and induced currents by use of a ringed dish as well as in a separate electric field exposure system. Measurements from 1 to 10 MHz will look for the effects of hyperfine transitions that are predicted for hydrogen atoms in the earth?s magnetic field near 45 ìT as a function of amplitudes, frequency, exposure times and static magnetic fields. The work will focus on the coupling between nuclear spins to the active electrons within the fragments of radical pairs and with biological cycle times, which can range from fractions of seconds to hours or longer. Radical concentration measurements will be made using fluorescent dyes for O-2 (super oxide), NADPH (nicotinamide adenine dinucleotide phosphate-oxidase) and H2O2 (hydrogen peroxide), and changes in membrane potentials will be examined. The new information gained as a result of the proposed work should help us understand exposure conditions to the fields from radar pulses, power lines, cell phones, Wi-Fi, TV, computers, etc., that may or may not lead to health effects. Broad impact will be achieved by providing an improved ability to specify exposure conditions that will inhibit or accelerate the growth of cells such as cancers reproducibly so that these fields can be used both experimentally and therapeutically. The project will result in the cross-training of graduate and undergraduate students at two universities: electrical engineering students at the University of Colorado will gain exposure to biology, chemistry and physics, while biomedical students at Western Michigan University will undertake engineering and physics practical experiences.